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The role of gibberellins in the regulation of Arabidopsis flowering time

Eriksson, Sven (2006). The role of gibberellins in the regulation of Arabidopsis flowering time. Diss. (sammanfattning/summary) Umeå : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2006:93
ISBN 91-576-7142-7
[Doctoral thesis]

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Abstract

The transition to flowering in the facultative long-day plant Arabidopsis (Arabidopsis thaliana) have been thoroughly studied and today it is well understood how flowering in response to changes in photoperiod and cold is integrated at the molecular level. However it is less known how flowering is initiated in response to internal factors in the absence of environmental stimuli. Previously it have been shown that flowering under non inductive short-day conditions is dependent on the plant hormone gibberellin (GA). In an effort to improve the understanding on how GA promotes flowering we have analysed, which of the many GA’s that are florally active, and quantified the developmental changes of florally active GA’s in relation to floral initiation under short-day conditions. We found that GA4 had the highest floral activity and that presiding floral initiation there was a dramatic increase at the shoot apex in the content of GA4. Intriguingly we found that the expression of the rate limiting GA 20-oxidase remained stable at the time when GA started to increase, indicating that increased accumulation of GA in not caused by local production. With the help of knockout lines we show that removal of either GA20ox1 or GA20ox2 delayed flowering and hence that both genes is participating in the production of GA relevant for the transition to flowering. Analysis of the flowering behaviour of GA2ox mutants revealed that some ga2ox mutants flowered earlier. Reporter gene analysis showed that expression of the GA2ox gene which had highest impact on flowering time was localised to the shoot apex, indicating that the function of the GA 2-oxidase is to control accumulation of GA’s in the shoot apex. Analysis of the effect of late flowering mutations on flowering time in plants with increased GA signal transduction showed that the LD pathway functions mostly in parallel to GA’s and that late flowering in autonomous mutants is partially due to decreased GA signal transduction or decreased GA synthetic capacity.

Authors/Creators:Eriksson, Sven
Title:The role of gibberellins in the regulation of Arabidopsis flowering time
Year of publishing :October 2006
Volume:2006:93
Number of Pages:46
Papers/manuscripts:
NumberReferences
ALLI. Böhlenius, H., Eriksson, S., & Nilsson, O. Regulation of LEAFY Transcription by Gibberellins and a Labile Repressor. (Manuscript) II. Eriksson, S. Böhlenius, H., Moritz, T. & Nilsson, O. GA4 is the Active Gibberellin in the Regulation of LEAFY Transcription and Arabidopsis Floral Initiation. Plant Cell 18, 2172-2181 III. Eriksson, S., Rieu, I., Nilsson, O., Phillips A. & Hedden, P. GA2ox4 Modulates the Level of Active GA to Control the Transition to Flowering in Arabidopsis. (Manuscript) IV. Eriksson, S. & Nilsson, O. Genetic Interactions between Gibberellin Signaling and Flowering Time Genes. (Manuscript)
Place of Publication:Umeå
ISBN for printed version:91-576-7142-7
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:arabidopsis thaliana, gibberelic acid, induced flowering, hormonal control, photoperiodicity
Keywords:Arabidopsis thaliana, Gibberellin, Flowering time, GA20ox, GA2ox, Sucrose, Floral initiation, photoperiod
URN:NBN:urn:nbn:se:slu:epsilon-1246
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-1246
ID Code:1244
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: Sven Eriksson
Deposited On:23 Oct 2006 00:00
Metadata Last Modified:02 Dec 2014 10:10

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