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A complete map of potential pathogenicity markers of avian influenza virus subtype H5 predicted from 11 expressed proteins

Khaliq, Zeeshan and Leijon, Mikael and Belak, Sandor and Komorowski, Jan (2015). A complete map of potential pathogenicity markers of avian influenza virus subtype H5 predicted from 11 expressed proteins. BMC Microbiology. 15:128, 1-13
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Official URL: http://dx.doi.org/10.1186/s12866-015-0465-x

Abstract

Background: Polybasic cleavage sites of the hemagglutinin (HA) proteins are considered to be the most important determinants indicating virulence of the avian influenza viruses (AIV). However, evidence is accumulating that these sites alone are not sufficient to establish high pathogenicity. There need to exist other sites located on the HA protein outside the cleavage site or on the other proteins expressed by AIV that contribute to the pathogenicity.Results: We employed rule-based computational modeling to construct a map, with high statistical significance, of amino acid (AA) residues associated to pathogenicity in 11 proteins of the H5 type viruses. We found potential markers of pathogenicity in all of the 11 proteins expressed by the H5 type of AIV. AA mutations S-43(HA1)-D, D-83(HA1)-A in HA; S-269-D, E-41-H in NA; S-48-N, K-212-N in NS1; V-166-A in M1; G-14-E in M2; K-77-R, S-377-N in NP; and Q-48-P in PB1-F2 were identified as having a potential to shift the pathogenicity from low to high. Our results suggest that the low pathogenicity is common to most of the subtypes of the H5 AIV while the high pathogenicity is specific to each subtype. The models were developed using public data and validated on new, unseen sequences.Conclusions: Our models explicitly define a viral genetic background required for the virus to be highly pathogenic and thus confirm the hypothesis of the presence of pathogenicity markers beyond the cleavage site.

Authors/Creators:Khaliq, Zeeshan and Leijon, Mikael and Belak, Sandor and Komorowski, Jan
Title:A complete map of potential pathogenicity markers of avian influenza virus subtype H5 predicted from 11 expressed proteins
Series/Journal:BMC Microbiology (1471-2180)
Year of publishing :2015
Volume:15
Number:128
Page range:1-13
Number of Pages:13
Publisher:BioMed Central
ISSN:1471-2180
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:L Animal production > L70 Veterinary science and hygiene - General aspects
L Animal production > L73 Animal diseases
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Medical Bioscience
Agrovoc terms:pathogenicity, avian influenzavirus, proteins
Keywords:avian Influenza virus, pathogenicity, virulence, MCFS, rosetta, rough sets
URN:NBN:urn:nbn:se:slu:epsilon-e-2818
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2818
Additional ID:
Type of IDID
Web of Science (WoS)000356912800001
DOI10.1186/s12866-015-0465-x
ID Code:12457
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
Deposited By: SLUpub Connector
Deposited On:30 Jul 2015 12:48
Metadata Last Modified:30 Jul 2015 12:48

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