Khaliq, Zeeshan and Leijon, Mikael and Belak, Sandor and Komorowski, Jan
(2015).
A complete map of potential pathogenicity markers of avian influenza virus subtype H5 predicted from 11 expressed proteins.
BMC Microbiology. 15
:128
, 1-13
[Journal article]
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Official URL: http://dx.doi.org/10.1186/s12866-015-0465-x
Abstract
Background: Polybasic cleavage sites of the hemagglutinin (HA) proteins are considered to be the most important determinants indicating virulence of the avian influenza viruses (AIV). However, evidence is accumulating that these sites alone are not sufficient to establish high pathogenicity. There need to exist other sites located on the HA protein outside the cleavage site or on the other proteins expressed by AIV that contribute to the pathogenicity.Results: We employed rule-based computational modeling to construct a map, with high statistical significance, of amino acid (AA) residues associated to pathogenicity in 11 proteins of the H5 type viruses. We found potential markers of pathogenicity in all of the 11 proteins expressed by the H5 type of AIV. AA mutations S-43(HA1)-D, D-83(HA1)-A in HA; S-269-D, E-41-H in NA; S-48-N, K-212-N in NS1; V-166-A in M1; G-14-E in M2; K-77-R, S-377-N in NP; and Q-48-P in PB1-F2 were identified as having a potential to shift the pathogenicity from low to high. Our results suggest that the low pathogenicity is common to most of the subtypes of the H5 AIV while the high pathogenicity is specific to each subtype. The models were developed using public data and validated on new, unseen sequences.Conclusions: Our models explicitly define a viral genetic background required for the virus to be highly pathogenic and thus confirm the hypothesis of the presence of pathogenicity markers beyond the cleavage site.
Authors/Creators: | Khaliq, Zeeshan and Leijon, Mikael and Belak, Sandor and Komorowski, Jan | ||||||
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Title: | A complete map of potential pathogenicity markers of avian influenza virus subtype H5 predicted from 11 expressed proteins | ||||||
Series/Journal: | BMC Microbiology (1471-2180) | ||||||
Year of publishing : | 2015 | ||||||
Volume: | 15 | ||||||
Number: | 128 | ||||||
Page range: | 1-13 | ||||||
Number of Pages: | 13 | ||||||
Publisher: | BioMed Central | ||||||
ISSN: | 1471-2180 | ||||||
Language: | English | ||||||
Publication Type: | Journal article | ||||||
Refereed: | Yes | ||||||
Article category: | Scientific peer reviewed | ||||||
Version: | Published version | ||||||
Full Text Status: | Public | ||||||
Agris subject categories.: | L Animal production > L70 Veterinary science and hygiene - General aspects L Animal production > L73 Animal diseases | ||||||
Subjects: | (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Medical Bioscience | ||||||
Agrovoc terms: | pathogenicity, avian influenzavirus, proteins | ||||||
Keywords: | avian Influenza virus, pathogenicity, virulence, MCFS, rosetta, rough sets | ||||||
URN:NBN: | urn:nbn:se:slu:epsilon-e-2818 | ||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2818 | ||||||
Additional ID: |
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ID Code: | 12457 | ||||||
Faculty: | VH - Faculty of Veterinary Medicine and Animal Science | ||||||
Department: | (VH) > Dept. of Biomedical Sciences and Veterinary Public Health | ||||||
Deposited By: | SLUpub Connector | ||||||
Deposited On: | 30 Jul 2015 12:48 | ||||||
Metadata Last Modified: | 09 Sep 2020 14:17 |
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