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Studies on plant-microbe interaction to improve stress tolerance in plants for sustainable agriculture

Asari, Shashidar Yadav (2015). Studies on plant-microbe interaction to improve stress tolerance in plants for sustainable agriculture. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2015:76
ISBN 978-91-576-8350-2
eISBN 978-91-576-8351-9
[Doctoral thesis]

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Abstract

Biotic and abiotic stress factors have a major impact on plants and cause extensive losses to crop production. Bacteria that provide growth promotion and prime stress tolerance of plants have great potential to improve crop production and support durable and environmental friendly resource management. Priming refers here to when plants upon appropriate stimulation develop an enhanced capacity to express defense responses to a later stimulus.

In this study strains of the beneficial bacterium Bacillus amyloliquefaciens were analysed for their effects on plants. Direct antagonistic effect of B. amyloliquefaciens on several Brassica phytopathogens (Botrytis cinerea, Alternaria brassicae, Alternaria brassicicola, Verticillium longisporum, and Sclerotinia sclerotiorum) was demonstrated by bacteria and exudates in vitro. A bacterial exudate fraction containing lipopeptide antibiotics was analysed and the strongest antagonistic activity was connected with a novel linear form of fengycin identified using mass spectrometry.

Growth promotion of Arabidopsis thaliana Col-0, coi1-16, jar1 and npr1 but not in myb72 plants by B. amyloliquefaciens UCMB5113 was demonstrated with increased shoot and root biomass and increased number of lateral roots and root hairs while the primary root growth decreased. Bacillus inoculation resulted in profound effects on various plant hormones that will affect a variety of plant functions. Growth promotion was also demonstrated in split dish experiments where Bacillus strains were sequestered from Arabidopsis plants indicating a role for volatile organic compounds (VOCs). Bacillus VOCs also caused growth suppression of several phytopathogens. GC-MS analysis identified a large number of compounds in the VOC blend and the composition of VOCs was dependent on the medium used for cultivation and the effects on the plant also varied.

Thus these Bacillus strains promote growth of plants and improve the survivability of plants exposed to biotic stress challenges by priming of stress tolerance. These findings can be extended to different crops to improve crop productivity under various environmental conditions.

Authors/Creators:Asari, Shashidar Yadav
Title:Studies on plant-microbe interaction to improve stress tolerance in plants for sustainable agriculture
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :2015
Depositing date:27 August 2015
Volume:2015:76
Number of Pages:55
Papers/manuscripts:
NumberReferences
IShashidar Asari, Delphine Debois, Edwin De Pauw, Marc Ongena, Sarosh Bejai, and Johan Meijer. Analysis of Bacillus amyloliquefaciens UCMB5113 exudates indicates multiple biological activities and support for plant protection against Brassica phytopathogens. (Manuscript submitted).
IIShashidar Asari, Danuše Tarkowská, Jakub Rolcík, David Velázquez Palmero, Sarosh Bejai, and Johan Meijer. Analysis of plant growth promoting properties of Bacillus amyloliquefaciens UCMB5113 using Arabidopsis thaliana as host plant. (Manuscript submitted).
IIIShashidar Asari, Mikael Agerlin Petersen, Staffan Matzén, Sarosh Bejai, and Johan Meijer. Multiple effects of Bacillus amyloliquefaciens volatile compounds: growth promotion in Arabidopsis thaliana and growth inhibition of Brassica phytopathogens. (Manuscript submitted).
Place of Publication:Uppsala
Publisher:Department of Plant Biology, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-8350-2
ISBN for electronic version:978-91-576-8351-9
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F60 Plant physiology and biochemistry
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Cell Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
Agrovoc terms:bacillus, biotic stress, volatile compounds, arabidopsis thaliana
Keywords:Plant-microbe interaction, stress tolerance, Bacillus amyloliquefaciens
URN:NBN:urn:nbn:se:slu:epsilon-e-2874
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2874
ID Code:12539
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Plant Biology (from 140101)
External funders:FORMAS
Deposited By: Mr Shashidar Yadav Asari
Deposited On:28 Aug 2015 10:02
Metadata Last Modified:07 Mar 2016 06:55

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