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Improved seed handling techniques for Juniperus polycarpos

implications for active restoration of degraded Juniper forests in Iran

Daneshvar, Abolfazl (2015). Improved seed handling techniques for Juniperus polycarpos. Diss. (sammanfattning/summary) Alnarp : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2015:82
ISBN 978-91-576-8362-5
eISBN 978-91-576-8363-2
[Doctoral thesis]

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Abstract

Juniperus polycarpos (K. Koch) is one of six native juniper forest tree species in Iran whose population size is declining continuously. The ultimate aim of the research presented in this thesis was to increase the regeneration potential of J. polycarpos by identifying factors influencing seed quality and develop methods that increase germination capacity and vigor. The research focused on impact of infection by dwarf mistletoe on reproductive output, methods for sorting unproductive seeds from seed lots, improving germination capacity and vigor through sorting techniques and by breaking seed dormancy. The results show that moderate level of infection significantly (p < 0.05) reduced mean number of cones per unit area of the host crown, increasing the number of damaged seeds, reducing seed size and germination capacity. For sorting viable seeds from non-viable seeds, three methods were applied: Incubation, Drying and Separation (IDS), modified specific gravity (SG) separation and near infrared spectroscopy (NIRS). For the IDS experiments, seven days incubation followed by nine hours of drying and sedimentation in pure water or 200 g sucrose /l water solution resulted in 75% and 82% sunken-viable seeds, respectively. For seeds soaked for 48 hours, SG separation in 600 g/l sucrose solution also resulted in 77% sunken-viable seeds. For both sorting techniques, only 4% of viable seeds were lost into the discarded floating fraction. NIR spectroscopy of individual seeds discriminated between viable and non-viable seeds of J. polycarpos with 98% and 100% accuracy, respectively based on spectral differences attributed to seed coat chemical composition and storage reserves. For finding optimal dormancy breaking treatment and subsequent stimulation of germination capacity and vigor of J. polycarpos seeds, different pretreatments were tested. The results showed warm stratification for sixteen weeks followed by twelve weeks of cold stratification resulted in 72% germination after 12 days compared with 16-week cold-stratification alone (42%) and the control (8%). Exogenous application of phytohormones alone or in combination with cold stratification resulted in less than 50% germination, though significantly (p < 0.01) higher than the control (8%). As a whole, the results have strong implication for active restoration by increasing seedling production potential in nurseries.
Keywords: dormancy, IDS, NIRS, seed sorting, stratification, seed quality, seed vigor
Author’s address: Abolfazl Daneshvar, SLU, Southern Swedish Forest Research Centre, P.O. Box 49, SE-230 53 Alnarp, Sweden.
E-mail: Abolfazl.Daneshvar@slu.se, Abolfazl_Daneshvar@yahoo.com

Authors/Creators:Daneshvar, Abolfazl
Title:Improved seed handling techniques for Juniperus polycarpos
Subtitle:implications for active restoration of degraded Juniper forests in Iran
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :3 September 2015
Depositing date:3 September 2015
Volume:2015:82
Number of Pages:66
Papers/manuscripts:
NumberReferences
IDaneshvar, A., Tigabu, M., Karimidoost, A., Farhadi, M., Oden, P.C. (2014). Growth characteristics and reproductive output of dwarf mistletoe-infected J. polycarpos in Iran. Journal of Forestry Research 25(4), 827-834.
IIDaneshvar, A., Tigabu, M., Karimidoost, A., Oden, P.C. Improved sorting system for upgrading the quality of J. polycarpos seed lots. (Submitted manuscript).
IIIDaneshvar, A., Tigabu, M., Karimidoost, A., Oden, P.C. Single seed NIR spectroscopy discriminates viable and non-viable seeds of J. polycarpos. Silva Fennica. 49 no. 5, article id 1334. 14 p.
IVDaneshvar, A., Tigabu, M., Karimidoost, A., Oden, P.C. Stimulation of germination in dormant seeds of J. polycarpos by stratification and hormone treatments (Submitted manuscript).
Place of Publication:Alnarp
Publisher:Southern Swedish Forest Research Centre, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-8362-5
ISBN for electronic version:978-91-576-8363-2
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F03 Seed production and processing
F Plant production > F62 Plant physiology - Growth and development
K Forestry > K10 Forestry production
Subjects:Obsolete subject words > FORESTRY, AGRICULTURAL SCIENCES and LANDSCAPE PLANNING > Plant production > Forestry
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Forest Science
Agrovoc terms:juniperus, forest trees, forests, seed, germination, seed dormancy, seed quality, grading, sorting equipment, parasitic plants, viscum album, infrared spectrophotometry, iran
Keywords:Greek juniper, conifers, forest health, semi parasite, seed size, seed viability, seed quality, seed vigor, seed sorting, warm-cold stratification, morpho-physiological dormancy, IDS, NIRS, OPLS, iran
URN:NBN:urn:nbn:se:slu:epsilon-e-2928
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2928
ID Code:12580
Faculty:S - Faculty of Forest Sciences
Department:(S) > Southern Swedish Forest Research Centre
Deposited By: Mr Abolfazl Daneshvar
Deposited On:14 Sep 2015 12:00
Metadata Last Modified:21 Dec 2016 23:08

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