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Molecular basis for odorant receptor tuning: a short C-terminal sequence is necessary and sufficient for selectivity of mosquito Or8

Hill, Sharon and Majeed, Shahid and Ignell, Rickard (2015). Molecular basis for odorant receptor tuning: a short C-terminal sequence is necessary and sufficient for selectivity of mosquito Or8. Insect molecular biology. 24:4, 491-501
[Journal article] (Unpublished)

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Abstract

A birth-and-death evolutionary model for odorant receptor gene repertoires presumes the creation of repertoires with the capacity for high-level diversity and rapid ligand specificity change. This changes the recognised odour space, directly affecting fitnessrelated behaviours and ultimately affecting adaptation to new environments and resources. The proximate molecular mechanisms underlying the tuning of odorant receptor repertoires, and thus peripheral olfaction, are unclear. In the present study, we report a concrete example of this model of odorant receptor evolution leading to rapid changes in receptor tuning that leave the peripheral neuronal circuitry intact. We identified a conserved odorant receptor gene in mosquitoes, Or8, which in Culex quinquefasciatus underwent a duplication and inversion event. The paralogues differ in only minor structural changes manifesting at the C-terminus. We assessed the specificity of the paralogous odorant receptors and receptor neurones. We found that the functional tuning of the receptor was indeed reflected in minor differences in amino acid structure. Specifically, we found that enantiomeric specificity of these mosquito Or8 paralogues relies on eight Cterminal amino acids encoded in the final exon of the gene; thus, the birth of a paralogous odorant receptor can change the tuning of the peripheral olfactory system.

Authors/Creators:Hill, Sharon and Majeed, Shahid and Ignell, Rickard
Title:Molecular basis for odorant receptor tuning: a short C-terminal sequence is necessary and sufficient for selectivity of mosquito Or8
Series/Journal:Insect molecular biology (1365-2583)
Year of publishing :2015
Volume:24
Number:4
Page range:491-501
Number of Pages:11
Publisher:John Wiley & Sons Ltd., The Royal Entomological Society
ISSN:1365-2583
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Evolutionary Biology
Keywords:olfaction, receptor-ligand interaction, selectivity, birth-and-death evolutionary model, insects, chemical ecology
URN:NBN:urn:nbn:se:slu:epsilon-e-2964
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-2964
Additional ID:
Type of IDID
Web of Science (WoS)000357883500009
DOI10.1111/imb.12176
ID Code:12633
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Protection Biology
External funders:Swedish Research Council
Deposited By: SLUpub Connector
Deposited On:05 Oct 2015 06:34
Metadata Last Modified:07 Feb 2016 00:07

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