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Sesame seed lignans

diversity, human metabolism and bioactivities

Moazzami, Ali (2006). Sesame seed lignans. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2006:98
ISBN 91-576-7147-8
[Doctoral thesis]

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Abstract

Sesame seed has been an important oilseed since ancient times. It contains 25% protein and 50% oil. Sesame seed possesses many health promoting effects, some of which have been attributed to a group of compounds called lignans (sesamin, sesamolin, sesaminol and sesamolinol). Sesame seed contains lignan aglycones in oil and lignan glucosides. Sesamin, the major sesame oil lignan, is known to reduce plasma cholesterol and to increase plasma γ-tocopherol in humans. This thesis aimed at investigating the content of lignan and lignan glucosides in sesame seeds, human metabolism of sesamin and the effect of possible interaction between sesamin and phytosterols on the bioactivity of sesamin. To these purposes, two new lignan glucosides, sesaminol diglucoside and sesamolinol diglucoside, were isolated from sesame seeds and characterized by LC-MS and NMR. A method for the analysis of sesame lignan glucosides was developed based on extraction of defatted sesame flour with 85% and 70% ethanol and using naringenin as internal standard and a reversed phase HPLC-DAD. A quantitative NMR technique was also developed to analyze the urinary metabolite of sesamin in humans based on using methylenedioxyphenyl signal (δ 5.91) in 1H-NMR spectra of deconjugated urine extracts to measure the metabolite(s) content. A diversity of sesame seed oil and products were analyzed for their content of lignans and lignan glucosides. It was shown that sesame seeds contain almost 0.6% of lignans and 32% of lignans are in glucosylated form of sesaminol and sesamolinol. In a human study, volunteers (n=6) consumed a single dose of muffins backed with sesame oil containing sesamin and sesamolin. It was shown that almost 30% of sesamin ingested is excreted in urine in the form of a major catechol metabolite. In an animal experiment, rats were fed diet containing two concentrations of sesamin and phytosterols. It was shown that phytosterols reduce the lipid modulating effects of sesamin on γ-tocopherol and dihomo-γ-linolenic acid. Overall, the findings from this thesis show that sesame seed is a rich source of lignans and that at least 30% of sesamin is absorbed in human, however the form of absorption is to be elucidated. Moreover, the biopotency of sesamin was reduced when the concentration of phytosterols was increased in the diet possibility because of reduction in the bioavailability of sesamin.

Authors/Creators:Moazzami, Ali
Title:Sesame seed lignans
Subtitle:diversity, human metabolism and bioactivities
Year of publishing :2006
Volume:2006:98
Number of Pages:62
Papers/manuscripts:
NumberReferences
ALLI. Moazzami, A.A., Andersson, R.E. & Kamal-Eldin, A. 2006. HPLC analysis of sesaminol glucosides in sesame seeds. Journal of Agricultural and Food Chemistry 54: 633-638. II. Moazzami, A.A., Andersson, R.E. & Kamal-Eldin, A. 2006. Characterization and analysis of sesamolinol diglucoside in sesame seeds. Bioscience, Biotechnology, and Biochemistry 70: 1478-1481. III. Moazzami, A.A. & Kamal-Eldin, A. 2006. Sesame seed is a rich source of dietary lignans. Journal of American Oil Chemist Society 83: 719-723. IV. Moazzami, A.A., Haese, S., Kamal-Eldin, A. 2006. Lignan contents in sesame seeds and sesame seed products. Manuscript. V. Moazzami, A.A., Andersson, R.E. & Kamal-Eldin, A. 2006. Human metabolism and urinary excretion of sesamin. Submitted VI. Moazzami, A.A., Frank, J., Lundh, T., Vessby, B & Kamal-Eldin, A. 2006. Phytosterols inhibit lipid modulating effects of sesamin in rats. Manuscript.
Place of Publication:Uppsala
ISBN for printed version:91-576-7147-8
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:sesame, seed, lignans, fatty acids, bioavailability, metabolism, tocopherols, phytosterols, cytochrome p450, rats, laboratory animals
Keywords:Sesame seed, lignans, sesamin, sesamolin, sesaminol, sesamolinol, human metabolism, bioavailability, rats, cytochrome P450, CYP, tocopherol, fatty acid.
URN:NBN:urn:nbn:se:slu:epsilon-1281
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-1281
ID Code:1264
Department:(NL, NJ) > Dept. of Food Science (until 161231)
Deposited By: Ali Moazzami
Deposited On:14 Nov 2006 00:00
Metadata Last Modified:02 Dec 2014 10:10

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