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Functional studies of RNA polymerase II-dependent transcription in yeast Saccharomyces cerevisiae

Tronnersjö, Susanna (2006). Functional studies of RNA polymerase II-dependent transcription in yeast Saccharomyces cerevisiae. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2006:109
ISBN 91-576-7258-X
[Doctoral thesis]

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Abstract

Nutrient availability is one of the environmental conditions that have the largest effect on cell growth. The nutrient status is transmitted by several signal transduction pathways, providing an essential framework for the coordination of cell growth. These pathways have been shown to be remarkably well conserved among eukaryotes. Transcriptional initiation is the endpoint for many signal transduction pathways and thus the final answer to an environmental change. The control of gene expression through transcription initiation is therefore one of the most fundamental processes in biology, containing many layers of complexity. I have used the model organism Saccharomyces cerevisiae to study different mechanisms involved in the control of gene expression. The mediator protein complex is required for regulated transcription of nearly all RNA polymerase II-dependent genes. Studies of the Mediator subunit Med21 revealed that it genetically and physically interacts with two other Mediator subunits, Med7 and Med10. A strong Med21 self-interaction was also identified, suggesting a possible dimerisation of Med21. The second part of the study focused on elucidating the function of the split jumonji domain, comprising the jmjN and jmjC subdomains, that has been identified in many transcription factors, by studying its physical interactions. I identified 19 different yeast proteins that could interact either directly or indirectly with the jumonji domain of the zinc finger protein Gis1 in a complex interaction pattern. Gis1 is known to be involved in nutrient dependent regulation of gene expression. Genome-wide microarray analysis was used to investigate the role of Gis1 and the related Rph1 protein in the growth phase-dependent gene regulation. This resulted in the identification of both redundant and unique roles of Gis1 and Rph1 in regulation of genes with an enrichment of STRE and PDS elements in their promoters. One of the proteins that interact directly with the jumonji domain of Gis1 is the unconvential prefoldin-like protein Bud27. Genetic and biochemical methods were used to study the roles of Bud27 and Gis1 in nutrient dependent regulation of gene expression by the TOR signaling pathway.

Authors/Creators:Tronnersjö, Susanna
Title:Functional studies of RNA polymerase II-dependent transcription in yeast Saccharomyces cerevisiae
Year of publishing :2006
Volume:2006:109
Number of Pages:68
Papers/manuscripts:
NumberReferences
ALLI. Hallberg, M., Hu, G. Z., Tronnersjö, S., Shaikhibrahim, Z., Balciunas, D. and Ronne, H. (2006) Functional and physical interactions within the middle domain of the yeast mediator. Mol. Gen. Genomics, 276:197-210 II. Tronnersjö, S., Hanefalk, C., Balciunas, D., Hu, G. Z., Nordberg, N., Murén, E. and Ronne, H. The jmjN and jmjC domains of the yeast zinc finger protein Gis1 interact with 19 proteins involved in transcription, sumoylation and DNA-repair. Mol. Gen. Genomics (in press). III. Orzechowski-Westholm, J., Tronnersjö, S., Nordberg, N., Komorowski, J. and Ronne, H. Role of the jmjC-containing zinc finger proteins Gis1 and Rph1 in growth phase-dependent gene expression in yeast. IV. Tronnersjö, S., Hu, G. Z., Nordberg, N. and Ronne, H. Functional studies of Bud27 and Gis1, two yeast proteins involved in TOR signaling.
Place of Publication:Uppsala
ISBN for printed version:91-576-7258-X
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:saccharomyces cerevisiae, yeasts, eucaryotic cells, gene expression, transcription, transcription factors, enzymes, genes
Keywords:Bud27, diauxic shift, Gis1, jmjC, jumonji, Mediator, nutrient signaling, PDS, rapamycin, Rph1, STRE, TOR, URI
URN:NBN:urn:nbn:se:slu:epsilon-1303
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-1303
ID Code:1266
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: Susanna Tronnersjö
Deposited On:17 Nov 2006 00:00
Metadata Last Modified:02 Dec 2014 10:10

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