Molecular regulation of vascular cambium identity and activity

Kucukoglu, Melis (2015). Molecular regulation of vascular cambium identity and activity. Diss. (sammanfattning/summary) Umeå : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2015:112
ISBN 978-91-576-8422-6
eISBN 978-91-576-8423-3
[Doctoral thesis]

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Abstract

In plants, secondary development and wood formation originates from the cell divisions within the vascular meristem, where the vascular stem cells are located. This thesis work presents my results on the molecular regulation of vascular cambium stem cell identity and activity.

I have investigated the role of the receptor-like kinase PXC1 during vascular development in Arabidopsis thaliana. Mutant analysis revealed that in the absence of PXC1, plants display a pendant phenotype and reduced secondary cell wall thickening and lignification in the inflorescence stems, showing that PXC1 is an important regulator of secondary cell wall formation in Arabidopsis.

I also participated in the characterization of members of the TDIF/CLE41/CLE44-TDR/PXY-WOX4 signaling module in hybrid aspen. Functional studies showed that knock down of PtWOX4 paralogs inhibits vascular cambium activity and secondary xylem formation in transgenic trees. Moreover, over-expression of PtCLE41A and related genes induces vascular patterning defects, highly associated with ectopic cambial activity. Results from transcriptional analysis suggested that PtCLE41A and related genes positively regulate PtWOX4 during the regulation of vascular cambium activity. By analysing gene expression patterns in Norway spruce, I provided evidence for the existence of conserved mechanisms in angiosperm and gymnosperm tree species with regard to the regulation of cambium function through CLE41, TDR/PXY and WOX4-like genes.

Finally, I also identified different PtLCLE genes as candidate regulators of vascular cambium activity and tree growth. I studied functions of these genes by employing transgenic approaches in hybrid aspen and showed that down regulation or up regulation of these genes affect many different phenotypic properties in hybrid aspen such as cambium activity, internode elongation, leaf size, and adventitious rooting.

In conclusion, results of these projects provide new insights into the regulation of vascular cambium activity and wood formation.

Authors/Creators:

Kucukoglu, Melis

Title:

Molecular regulation of vascular cambium identity and activity

Series/Journal:

Acta Universitatis agriculturae Sueciae (1652-6880)

Year of publishing :

November 2015

Depositing date:

4 November 2015

Volume:

2015:112

Number of Pages:

Papers/manuscripts:

Number	References
I	Wang J., Kucukoglu M., Zhang L., Chen P., Decker D., Nilsson O., Jones B., Sandberg G. & Zheng B. (2013). The Arabidopsis LRR-RLK, PXC1, is a regulator of secondary wall formation correlated with the TDIFPXY/TDR-WOX4 signaling pathway. BMC Plant Biology 13: 94
II	Kucukoglu M. , Nilsson J. , Zheng B., Sandberg G. & Nilsson O.(2015). WOX4-like genes control stem secondary growth in trees. (Manuscript).
III	Nystedt B., Street N.R., Wetterbom A., Zuccolo A., Lin Y.C., Scofield D.G., Vezzi F., Delhomme N., Giacomello S., Alexeyenko A., Vicedomini R., Sahlin K., Sherwood E., Elfstrand M., Gramzow L., Holmberg K., Hällman J., Keech O., Klasson L., Koriabine M., Kucukoglu M., Käller M., Luthman J., Lysholm F., Niittylä T., Olson A., Rilakovic N., Ritland C., Rosselló J.A., Sena J., Svensson T., Talavera-López C., Theißen G., Tuominen H., Vanneste K., Wu Z.Q., Zhang B., Zerbe P., Arvestad L., Bhalerao R., Bohlmann J., Bousquet J., Garcia Gil R., Hvidsten T.R., de Jong P., MacKay J., Morgante M., Ritland K., Sundberg B., Thompson S.L., Van de Peer Y., Andersson B., Nilsson O., Ingvarsson P.K., Lundeberg J. & Jansson S. (2013). The Norway spruce genome sequence and conifer genome evolution. Nature 497: 579-584
IV	Kucukoglu M., Chaabouni S., Zheng B. & Nilsson O. (2015). Functional characterization of CLE genes expressed in the wood-forming zone of Populus trees. (Manuscript).
V	Kucukoglu M. & Nilsson O. (2015). CLE peptide signaling in plants – the power of moving around. Physiologia Plantarum 155: 74-87.
UNSPECIFIED	* These authors contributed equally to this work.

Place of Publication:

Umeå

Publisher:

Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences

ISBN for printed version:

978-91-576-8422-6

ISBN for electronic version:

978-91-576-8423-3

ISSN:

1652-6880

Language:

English

Publication Type:

Doctoral thesis

Full Text Status:

Public

Agris subject categories.:

F Plant production > F30 Plant genetics and breeding
F Plant production > F50 Plant structure
F Plant production > F62 Plant physiology - Growth and development

Subjects:

(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Developmental Biology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology

Agrovoc terms:

populus, arabidopsis, picea, wood, lignification, cambium, cell walls, genetic engineering, transcription factors, ligands, molecular biology

Keywords:

vascular cambium, stem cells, secondary growth, wood formation, Populus, PtWOX4, PtLCLE

URN:NBN:

urn:nbn:se:slu:epsilon-e-3048

Permanent URL:

http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3048

ID Code:

12771

Faculty:

S - Faculty of Forest Sciences

Department:

(S) > Dept. of Forest Genetics and Plant Physiology

External funders:

FORMAS and Kempe Foundations and Knut and Alice Wallenberg Foundation and Swedish Research Council and Swedish Agency for Innovation Systems

Deposited By:

Melis Kucukoglu

Deposited On:

09 Nov 2015 07:37

Metadata Last Modified:

15 Dec 2015 09:59

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Molecular regulation of vascular cambium identity and activity

Abstract