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Inflammatory response in equine airways

cytokines in bronchial epithelium and bronchoalveolar lavage cells

Riihimäki, Miia (2008). Inflammatory response in equine airways. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 ; 2008:12
ISBN 978-91-85913-45-9
[Doctoral thesis]

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Non-infectious airway inflammation in horses is a common problem worldwide when horses are housed indoors. In this thesis the inflammatory pathways in equine airways, with special regards to the role of bronchial epithelium, were investigated in horses with highly reactive airways (recurrent airway obstruction, RAO) and in clinically normal race horses under conventional management. In the former group the cytokine mRNA and protein expression in bronchial epithelium and were examined, whereas only the cytokine mRNA expression of bronchoalveolar lavage (BAL) cells and BAL cytology were included in the work on the stabled race horses. These latter horses unexpectedly developed transient pulmonary eosinophilia which allowed deeper investigation of eosinophil kinetics in horses. These studies showed that endobronchial biopsy was a safe and readily performed procedure to obtain airway tissue samples from the live horse. Moreover, biopsies were of sufficient size and quality for evaluation of cytokine mRNA expression, airway morphology and for immunohistochemical analysis. The work on RAO horses strengthened earlier findings of increased mRNA expression of the neutrophil chemotaxic cytokine IL-8 in RAO horses in both BAL cell and bronchial tissues. Thus, use of bronchial tissues can provide a valued complementary tool to study serial events of inflammation in equine airway at closely timed intervals not possible with the sampling technique of BAL. In the group of race horses, prolonged winter stabling was associated with subtlepulmonary alterations reflective of increased inflammatory response in equine airways.Specifically there was increased expression of IL-6 mRNA in BAL cells along with a trend for increased neutrophils in the BAL. No underlying cause of the transient pulmonary eosinophilia could be identified. Surprisingly, expression of mRNA of eosinophil associated IL-4 and IL-5 was not detectably altered in these horses. It appears that eosinophil influx to the equine lung can be subclinical apart from temporary exercise intolerance, and abate without specific treatment other than deworming. These combined studies introduce and demonstrate future potential in examination of bronchial tissues as a complement to BAL cells studies in molecular biological analysis to increase the understanding of mechanisms of noninfectious airway inflammation in the horse.

Authors/Creators:Riihimäki, Miia
Title:Inflammatory response in equine airways
Subtitle:cytokines in bronchial epithelium and bronchoalveolar lavage cells
Series Name/Journal:Acta Universitatis Agriculturae Sueciae
Year of publishing :2008
Number of Pages:81
ALLI. M. Riihimäki, A. Raine, T. Art, P. Lekeux, L. Couëtil and J. Pringle. Partial divergence of cytokine mRNA expression in bronchial tissues compared to bronchoalveolar lavage cells in horses with recurrent airway obstruction. Vet Immunol Immunopathol. In press: 2008. doi:10.1016/j.vetimm.2007.12.001 II. M. Riihimäki, A. Raine, J. Pourazar, T. Sandström, T. Art, P. Lekeux, L. Couëtil and J. Pringle. Epithelial expression of mRNA and protein for IL-6, IL-10 and TNF-α in endobronchial biopsies in horses with Recurrent Airway Obstruction. BMC Veterinary Research. In press: 2008, 4:8, doi:10.1186/1746-6148-4-8 III. M. Riihimäki, I. Lilliehöök, A. Raine, M. Berg and J. Pringle. Clinical alterations and bronchoalveolar cell gene expression of mRNA IL-4 and IL-5 in horses with transient pulmonary eosinophilia. Res Vet Sci. In press: 2007. doi:10.1016/j.rvsc.2007.09.015 IV. M. Riihimäki, A. Raine, L. Elfman and J. Pringle. Markers of respiratory inflammation in horses in relation to seasonal changes in air quality in a conventional racing stable. Accepted. Can J Vet Res, 2008.
Place of Publication:Uppsala
ISBN for printed version:978-91-85913-45-9
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:horses, respiratory diseases, stables, cytokines, biopsy, disease control, animal diseases
Keywords:RAO, IAD, BAL, endobronchial biopsy, cytokine, horse, stable environment
Permanent URL:
ID Code:1279
Department:(VH) > Dept. of Clinical Sciences
Deposited By: Miia Riihimäki
Deposited On:05 Mar 2008 00:00
Metadata Last Modified:02 Dec 2014 10:11

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