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Insulin-like growth factor-I in the domestic cat

studies in health and disease

Strage, Emma (2015). Insulin-like growth factor-I in the domestic cat. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2015:124
ISBN 978-91-576-8446-2
eISBN 978-91-576-8447-9
[Doctoral thesis]

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Abstract

Insulin-like growth factor-I (IGF-I) has growth promoting effects as well as insulin-like actions on metabolism.
IGF-I associates with a family of six high affinity IGF-binding proteins (IGFBPs) and these interfere in immunoassays for IGF-I. The role of binding of IGF-I to IGFBP-3, together with a third protein (the acid-labile subunit), to form a high molecular mass ternary complex, is not known in cats. In adult humans the ternary complex is the dominant circulating form. The cat is a strict carnivore with different metabolism to other species, which may include differences in the IGF system. In clinical practice serum IGF-I is used routinely for screening for acromegaly in cats with diabetes mellitus (DM). The overall aim of this thesis was to determine factors regulating
IGF-I concentrations in health and disease.

Enzyme-linked immunosorbent assays for measuring feline IGF-I and insulin were validated. It is recommended that laboratories, in validating their assays, should be aware of position effects on assay plates and, for IGF-I assays, interference by circulating IGFBPs. For IGF-I, between animal variation was high (~65%) while within animal variation was considerable lower (~8%). These values for biological variation can now be used in interpreting clinical results after repeated sampling in screening for, and in the management of, acromegaly.

IGF-I concentrations were related to the amount of the ternary complex in healthy and diabetic cats. The ternary complex was the dominating circulating form only in cats with high IGF-I concentrations. There was a wide range of IGF-I concentrations in both healthy and diabetic cats that was in part related to variation in weight.

When using IGF-I as a screening tool for acromegaly in diabetic cats, glycaemic control should also be taken into consideration. IGF-I concentrations increased in response to insulin treatment and concentrations at 2-4 weeks were higher in cats that later went into remission.

In conclusion, in contrast to adult humans, circulating IGF-binding forms vary across the wide range of IGF-I concentrations in the cat. It is recommended that reference intervals for healthy cats are developed, stratifying by weight. IGF-I shows promise as a predictive marker for remission in feline diabetes mellitus.

Authors/Creators:Strage, Emma
Title:Insulin-like growth factor-I in the domestic cat
Subtitle:studies in health and disease
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :November 2015
Depositing date:12 November 2015
Volume:2015:124
Number of Pages:75
Papers/manuscripts:
NumberReferences
IStrage EM, Ström Holst B, Nilsson G, Jones B, Lilliehöök I (2012). Validation of an enzyme-linked immunosorbent assay for measurement of feline serum insulin. Veterinary Clinical Pathology, 41(4), 518-528.
IIStrage EM, Theodorsson E, Ström Holst B, Lilliehöök I, Lewitt MS (2015). Insulin-like growth factor-I in cats: validation of an enzyme-linked immunosorbent assay and determination of biologic variation. Veterinary Clinical Pathology, E-pub, September 29th. DOI:10.1111/vcp.12289.
IIIStrage EM, Fall T, Lewitt MS. Distribution of circulating IGF-binding protein complexes is similar in healthy and diabetic cats. Manuscript.
IVStrage EM, Sundberg M, Ström Holst B, Andersson Franko M, Ramström M, Fall T*, Lewitt MS*. Insulin-like growth factor I in cats with diabetes mellitus during insulin treatment: a longitudinal study. Manuscript. *Denotes equal contribution
Place of Publication:Uppsala
Publisher:Department of Clinical Sciences, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-8446-2
ISBN for electronic version:978-91-576-8447-9
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:L Animal production > L52 Animal physiology - Growth and development
L Animal production > L73 Animal diseases
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Agrovoc terms:cats, insulin-like growth factor, growth, insulin, diabetes, pituitary gland, endocrine diseases, diagnosis
Keywords:Acid-labile subunit, Acromegaly, Diabetes mellitus, Growth hormone, Insulin, Insulin-like growth factor binding proteins, Ternary complex
URN:NBN:urn:nbn:se:slu:epsilon-e-3064
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3064
ID Code:12810
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
External funders:Agria and Michael Forsgrens stiftelse and Stift Gymn Dir Stina Johanssons i Fränsta Fond F and Swedish Veterinary Association
Deposited By: Emma Strage
Deposited On:12 Nov 2015 14:56
Metadata Last Modified:07 Mar 2016 12:16

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