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Characterization of regulatory factors controlling tip growth in Nicotiana tabacum pollen tubes

Sun, Jia (2015). Characterization of regulatory factors controlling tip growth in Nicotiana tabacum pollen tubes. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2015:107
ISBN 978-91-576-8412-7
eISBN 978-91-576-8413-4
[Doctoral thesis]

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Abstract

Rho GTPases constitute a family of highly conserved signaling proteins with key functions in the regulation of cellular polarization and polar cell growth. In plants, Rho GTPases are represented by a plant specific subfamily referred to as Rac/Rop GTPases. Some members of this subfamily associate with the plasma membrane specifically at the tip of pollen tubes and are important regulators of polar cell expansion. The aim of my study has been to improve our understanding of the molecular mechanisms that control the activity of pollen tube Rac/Rop, and the signaling downstream of this activity.

BiFC (Bimolecular Fluorescence Complementation) is a technique used to validate protein-protein interactions. My studies on the interactions between Nt-RhoGAP1/ RhoGDI2 and Nt-Rac5 show that 35S promoter regulated BiFC works in normally growing N. tabacum pollen tubes and that the intracellular interaction sites between Nt-RhoGAP1/ RhoGDI2 and Nt-Rac5 are found at the subapical plasma membrane and apical cytoplasm, respectively. This technique was consequently used to confirm potential interacting protein partners involved in Nt-Rac5 signaling networks (i.e. Nt-Risap and Nt-Ric), and to detect the subcellular localization of these interactions in pollen tubes.

Nt-Risap (Rac5 interacting sub-apical protein) and Ric (Rho interacting CRIB containing) were isolated as effectors of Nt-Rac5 in yeast two-hybrid screens. The two proteins were confirmed to be highly and specifically expressed in N. tabacum pollen tubes and to interact with Nt-Rac5 both in vitro and in vivo. Functional studies of the two proteins suggest that they behave as downstream effectors of Nt-Rac5 and are involved in the Nt-Rac5 signaling pathways that regulate pollen tube tip growth by activating specific downstream events.

Authors/Creators:Sun, Jia
Title:Characterization of regulatory factors controlling tip growth in Nicotiana tabacum pollen tubes
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :November 2015
Depositing date:16 November 2015
Volume:2015:107
Number of Pages:52
Papers/manuscripts:
NumberReferences
ISun, J., Eklund, D.M., Montes-Rodriguez, A & Kost, B. (2015). In vivo Rac/Rop localization as well as interaction with RhoGAP and RhoGDI in tobacco pollen tubes: analysis by low-level expression of fluorescent fusion proteins and bimolecular fluorescence complementation. The Plant Journal 84, 83-98.
IIStephan, O., Cottier, S., Fahlén, S., Montes-Rodriguez, A., Sun, J., Eklund, D.M., Klahre, U & Kost, B. (2014). RISAP is a TGN-associated RAC5 effector regulating membrane traffic during polar cell growth in tobacco. The Plant Cell 26, 4426–4447.
IIISun, J., Stephan, O., Fahlén, S., Eklund, D.M., Klahre, U & Kost, B. Functional characterization of Nt-Ric, an effector of Nt-Rac5 in tobacco pollen tubes. (Manuscript).
Place of Publication:Uppsala
Publisher:Department of Plant Biology, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-8412-7
ISBN for electronic version:978-91-576-8413-4
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F60 Plant physiology and biochemistry
F Plant production > F62 Plant physiology - Growth and development
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Cell Biology
Agrovoc terms:nicotiana tabacum, pollen tubes, growth, proteins, enzymes
Keywords:pollen tube, tip growth, Rho GTPase
URN:NBN:urn:nbn:se:slu:epsilon-e-3088
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3088
ID Code:12822
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Plant Biology (from 140101)
Deposited By: mrs jia sun
Deposited On:24 Nov 2015 12:43
Metadata Last Modified:17 Dec 2015 16:18

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