Nemesio Gorriz, Miguel
(2015).
Molecular responses to Heterobasidion annosum s.l. in Picea abies.
Diss. (sammanfattning/summary)
Uppsala :
Sveriges lantbruksuniv.,
Acta Universitatis Agriculturae Sueciae, 1652-6880
; 2015:114
ISBN 978-91-576-8426-4
eISBN 978-91-576-8427-1
[Doctoral thesis]
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Abstract
Norway spruce [Picea abies (L.) Karst.] is a main tree species in European forests and is important both ecologically and economically. The root rot fungus Heterobasidion annosum sensu lato (s.l.) is the main P. abies pathogen. Including resistance in breeding programs would help mitigating the impact of the pathogen but knowledge regarding defense mechanisms in P. abies needs a better understanding. The work within this thesis intended to expand the existing knowledge on P. abies resistance mechanisms focusing on hormone signaling, flavonoid biosynthesis and its transcriptional regulation. I found that jasmonic acid is the major hormone controlling defense signaling pathways in P. abies. Furthermore, we validated a candidate gene, PaLAR3, as a resistance marker for H. annosum s.l. in P. abies. PaLAR3 encodes an enzyme responsible for the synthesis of (+) catechin, which showed a fungistatic effect on H. parviporum. Analysis of genetic diversity revealed two allelic lineages of PaLAR3 that showed significant differences in fungal resistance and (+) catechin content that were explained by dissimilarities in inducibility. We studied the role of PaNAC03, a transcription factor that is associated with H. annosum s.l. infection. PaNAC03 not only showed repression of multiple genes including PaLAR3, but bound only to the promoter of one of the PaLAR3 allelic lineages explaining at least partly the differences in allelic expression that were observed. Finally, we identified a full repertoire of members of a MYB/bHLH/WDR transcription factor complex in Norway spruce, which showed differences in protein interactions and expression patterns, and also in ability to control the expression of genes in the flavonoid biosynthetic pathway including PaLAR3.
Authors/Creators: | Nemesio Gorriz, Miguel | ||||||||||
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Title: | Molecular responses to Heterobasidion annosum s.l. in Picea abies | ||||||||||
Series/Journal: | Acta Universitatis Agriculturae Sueciae (1652-6880) | ||||||||||
Year of publishing : | 14 December 2015 | ||||||||||
Depositing date: | 9 December 2015 | ||||||||||
Number: | 2015:114 | ||||||||||
Number of Pages: | 74 | ||||||||||
Papers/manuscripts: |
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Place of Publication: | Uppsala | ||||||||||
Publisher: | Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences | ||||||||||
ISBN for printed version: | 978-91-576-8426-4 | ||||||||||
ISBN for electronic version: | 978-91-576-8427-1 | ||||||||||
ISSN: | 1652-6880 | ||||||||||
Language: | English | ||||||||||
Publication Type: | Doctoral thesis | ||||||||||
Full Text Status: | Public | ||||||||||
Agris subject categories.: | H Protection of plants and stored products > H20 Plant diseases K Forestry > K01 Forestry - General aspects | ||||||||||
Subjects: | (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Forest Science (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding | ||||||||||
Agrovoc terms: | picea abies, heterobasidion annosum, pathogens, root rots, defence mechanisms, genes, gene expression, plant growth substances, hormones, flavonoids, transcription factors | ||||||||||
Keywords: | Picea abies, Norway spruce, Heterobasidion, defense, phytohormones, flavonoid, catechin, LAR, MYB, NAC, promoter | ||||||||||
URN:NBN: | urn:nbn:se:slu:epsilon-e-3128 | ||||||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3128 | ||||||||||
ID Code: | 12902 | ||||||||||
Faculty: | S - Faculty of Forest Sciences | ||||||||||
Department: | (NL, NJ) > Dept. of Forest Mycology and Plant Pathology (S) > Dept. of Forest Mycology and Plant Pathology | ||||||||||
Deposited By: | Miguel Nemesio Gorriz | ||||||||||
Deposited On: | 10 Dec 2015 16:05 | ||||||||||
Metadata Last Modified: | 10 Sep 2020 13:41 |
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