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Molecular responses to Heterobasidion annosum s.l. in Picea abies

Nemesio Gorriz, Miguel (2015). Molecular responses to Heterobasidion annosum s.l. in Picea abies. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2015:114
ISBN 978-91-576-8426-4
eISBN 978-91-576-8427-1
[Doctoral thesis]

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Abstract

Norway spruce [Picea abies (L.) Karst.] is a main tree species in European forests and is important both ecologically and economically. The root rot fungus Heterobasidion annosum sensu lato (s.l.) is the main P. abies pathogen. Including resistance in breeding programs would help mitigating the impact of the pathogen but knowledge regarding defense mechanisms in P. abies needs a better understanding. The work within this thesis intended to expand the existing knowledge on P. abies resistance mechanisms focusing on hormone signaling, flavonoid biosynthesis and its transcriptional regulation. I found that jasmonic acid is the major hormone controlling defense signaling pathways in P. abies. Furthermore, we validated a candidate gene, PaLAR3, as a resistance marker for H. annosum s.l. in P. abies. PaLAR3 encodes an enzyme responsible for the synthesis of (+) catechin, which showed a fungistatic effect on H. parviporum. Analysis of genetic diversity revealed two allelic lineages of PaLAR3 that showed significant differences in fungal resistance and (+) catechin content that were explained by dissimilarities in inducibility. We studied the role of PaNAC03, a transcription factor that is associated with H. annosum s.l. infection. PaNAC03 not only showed repression of multiple genes including PaLAR3, but bound only to the promoter of one of the PaLAR3 allelic lineages explaining at least partly the differences in allelic expression that were observed. Finally, we identified a full repertoire of members of a MYB/bHLH/WDR transcription factor complex in Norway spruce, which showed differences in protein interactions and expression patterns, and also in ability to control the expression of genes in the flavonoid biosynthetic pathway including PaLAR3.

Authors/Creators:Nemesio Gorriz, Miguel
Title:Molecular responses to Heterobasidion annosum s.l. in Picea abies
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :14 December 2015
Depositing date:9 December 2015
Volume:2015:114
Number of Pages:74
Papers/manuscripts:
NumberReferences
IArnerup J., Nemesio-Gorriz M., Lundén K., Asiebgu F., Stenlid J. and Elfstrand M. (2013). The primary module in Norway spruce defense signaling against H. annosum s.l. seems to be jasmonate-mediated signaling without antagonism of salicylate-mediated signaling. Planta, 237/4, pp. 1037-1045.
IINemesio-Gorriz M., Hammerbacher A., Ihrmark K., Källman T., Olson Å., Lascoux M., Stenlid J., Gershenzon J. and Elfstrand M. (2015). Different alleles of a gene encoding leucoanthocyanidin reductase (LAR3) influence resistance against the fungus Heterobasidion parviporum in Picea abies (submitted).
IIINemesio-Gorriz M., Blair P.B., Dalman K., Hammerbacher A., Arnerup J., Stenlid J., Muhktar S.M. and Elfstrand M. Identification of Norway spruce WDR and bHLH proteins forming complexes with MYB transcription factors that regulate gene expression in the flavonoid pathway (manuscript).
IVDalman K., Wind J., Nemesio-Gorriz M., Hammerbacher A., Lundén K., Ezcurra I. and Elfstrand M. Overexpression of PaNAC03, a stress induced NAC gene family transcription factor in Norway spruce leads to reduced flavonol biosynthesis (manuscript).
Place of Publication:Uppsala
Publisher:Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-8426-4
ISBN for electronic version:978-91-576-8427-1
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:H Protection of plants and stored products > H20 Plant diseases
K Forestry > K01 Forestry - General aspects
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Forest Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding
Agrovoc terms:picea abies, heterobasidion annosum, pathogens, root rots, defence mechanisms, genes, gene expression, plant growth substances, hormones, flavonoids, transcription factors
Keywords:Picea abies, Norway spruce, Heterobasidion, defense, phytohormones, flavonoid, catechin, LAR, MYB, NAC, promoter
URN:NBN:urn:nbn:se:slu:epsilon-e-3128
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3128
ID Code:12902
Faculty:S - Faculty of Forest Sciences
Department:(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology
Deposited By: Miguel Nemesio Gorriz
Deposited On:10 Dec 2015 16:05
Metadata Last Modified:24 Dec 2015 13:53

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