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High-throughput sequencing reveals drastic changes in fungal communities in the phyllosphere of Norway spruce (Picea abies) following invasion of the spruce bud scale (Physokermes piceae)

Menkis, Audrius and Adas, Marčiulynas and Gedminas, Artūras and Lynikienė, Jūratė and Povilaitienė, Aistė (2015). High-throughput sequencing reveals drastic changes in fungal communities in the phyllosphere of Norway spruce (Picea abies) following invasion of the spruce bud scale (Physokermes piceae). Microbial ecology : an international journal. 70:4, 904-911
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Official URL: http://dx.doi.org/10.1007/s00248-015-0638-z

Abstract

The aim of this study was to assess the diversity and composition of fungal communities in damaged and undamaged shoots of Norway spruce (Picea abies) following recent invasion of the spruce bud scale (Physokermes piceae) in Lithuania. Sampling was done in July 2013 and included 50 random lateral shoots from ten random trees in each of five visually undamaged and five damaged 40-50-year-old pure stands of P. abies. DNA was isolated from 500 individual shoots, subjected to amplification of the internal transcribed spacer of fungal ribosomal DNA (ITS rDNA), barcoded and sequenced. Clustering of 149,426 high-quality sequences resulted in 1193 non-singleton contigs of which 1039 (87.1 %) were fungal. In total, there were 893 fungal taxa in damaged shoots and 608 taxa in undamaged shoots (p < 0.0001). Furthermore, 431 (41.5 %) fungal taxa were exclusively in damaged shoots, 146 (14.0 %) were exclusively in undamaged shoots, and 462 (44.5 %) were common to both types of samples. Correspondence analysis showed that study sites representing damaged and undamaged shoots were separated from each other, indicating that in these fungal communities, these were largely different and, therefore, heavily affected by P. piceae. In conclusion, the results demonstrated that invasive alien tree pests may have a profound effect on fungal mycobiota associated with the phyllosphere of P. abies, and therefore, in addition to their direct negative effect owing physical damage of the tissue, they may also indirectly determine health, sustainability and, ultimately, distribution of the forest tree species.

Authors/Creators:Menkis, Audrius and Adas, Marčiulynas and Gedminas, Artūras and Lynikienė, Jūratė and Povilaitienė, Aistė
Title:High-throughput sequencing reveals drastic changes in fungal communities in the phyllosphere of Norway spruce (Picea abies) following invasion of the spruce bud scale (Physokermes piceae)
Series/Journal:Microbial ecology : an international journal (0095-3628)
Year of publishing :2015
Depositing date:21 December 2015
Volume:70
Number:4
Page range:904-911
Number of Pages:8
Publisher:Springer
ISSN:0095-3628
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Accepted version
Full Text Status:Public
Agris subject categories.:H Protection of plants and stored products > H10 Pests of plants
K Forestry > K70 Forest injuries and protection
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Ecology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Forest Science
Agrovoc terms:forest health, pathogens, pest insects
Keywords:Forest health, Pathogens, Pest insects, Climate change, Fungal community
URN:NBN:urn:nbn:se:slu:epsilon-e-3142
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3142
Additional ID:
Type of IDID
Web of Science (WoS)000363952600005
DOI10.1007/s00248-015-0638-z
ID Code:12921
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
S - Faculty of Forest Sciences
Department:(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology
Deposited By: SLUpub Connector
Deposited On:21 Dec 2015 09:29
Metadata Last Modified:09 Jun 2016 23:15

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