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Defence related molecular signalling in potato

new perspectives from “- Omics”

Burra, Dharani Dhar (2016). Defence related molecular signalling in potato. Diss. (sammanfattning/summary) Alnarp : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 ; 2016:7
ISBN 978-91-576-8516-2
eISBN 978-91-576-8517-9
[Doctoral thesis]

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Potato production is hampered by several pathogens and is subjected to intense chemical based disease control, use of which also has undesirable consequences. Resistance breeding programs have also shown limited success. Hence, there is a need to develop durable disease resistance. Omics-techniques enable new layers of knowledge regarding molecules and their interactions mediating defence, which can contribute to identification of durable resistance sources.

A novel network-based approach was used to improve the existing annotation of gene probes on the genome based microarray. Approximately 8000 unannotated probes received a new annotation. This improved annotation was used to assess genome wide changes in transcripts and proteins in response to treatments with resistance inducers, β- amino butyric acid (BABA) and Phosphite based salt (Phi). Five thousand transcripts were significantly regulated 48 hours after 10mM BABA treatment while one was regulated with 1mM BABA. In coherence, 10 mM BABA but not 1 mM induced protection to the hemibiotroph Phytophthora infestans. No transcript was significantly regulated 48 hours after Phi treatment. Time course analysis revealed that Phi exerts a transient effect, as significant transcriptomic changes were observed only 3, 6 and 11 hours after treatment. In contrast, plants showed resistance to P. infestans even at 120 hours after Phi treatment. Phi and BABA dependent “Induced state” is not restricted to transcripts related to plant defence, as transcripts related to abiotic stress and primary metabolism were altered, while biotic stress and cell wall related proteins also increased in abundance.
Furthermore, an in vitro based blackleg disease screening assay was developed to investigate Potato – Dickeya solani interactions. We show that salicylic (SA) and COI1 are necessary for defence in shoots and tubers to this necrotroph. We also screened a crossing population and identified “potential” D. solani susceptibility genes related to transcriptional regulation. We also show that while SA is necessary to restrict lesion development and pathogen growth in response to the necrotroph Alternaria solani, COI1 affects pathogen growth only. Transcriptomic analysis indicated that rapid defence response to A. solani involves biotic, abiotic and oxidative stress related transcripts regulated by SA and COI1. We identified a citrate binding protein, which is also induced by resistance inducers, as an SA-repressed susceptibility factor to A. solani. Finally, proteomics of PAMP triggered immunity revealed upregulation of oxidative stress proteins while proteins related to oxidative stress tolerance, GTP binding activity were specifically upregulated in effector triggered immunity interactions.

Authors/Creators:Burra, Dharani Dhar
Title:Defence related molecular signalling in potato
Subtitle:new perspectives from “- Omics”
Series Name/Journal:Acta Universitatis Agriculturae Sueciae
Year of publishing :21 January 2016
Depositing date:12 January 2016
Number of Pages:85
IBengtsson, T., Weighill, D., Proux-Wéra, E., Levander, F., Resjö, S., Burra, D.D., Moushib, L.I., Hedley, P.E., Liljeroth, E., Jacobson, D. and Alexandersson, E., 2014. Proteomics and transcriptomics of the BABA-induced resistance response in Potato using a novel functional annotation approach. BMC genomics, 15(1): 315
IIBurra, D.D., Berkowitz, O., Hedley, P.E., Morris, J., Resjö, S., Levander, F., Liljeroth, E., Andreasson, E. and Alexandersson, E., 2014. Phosphite-induced changes of the transcriptome and secretome in Solanum tuberosum leading to resistance against Phytophthora infestans. BMC plant biology, 14(1),:254.
IIIBurra, D. D., Mühlenbock, P., Andreasson, E. (2015), Salicylic and jasmonic acid pathways are necessary for defence against Dickeya solani as revealed by a novel method for Blackleg disease screening of in vitro grown Potato. Plant Biology, 17: 1030–1038
IVBurra, D.D., Kushwaha, K.S., Alexandersson. E., Mühlenbock. P., Andreasson, E. Potential blackleg disease susceptibility factors revealed in potato by RNAseq analysis of a crossing population (Manuscript)
VOdilbekov, F*., Burra, D.D*., Rosahl, S., Hedley, PE., Morris, J., Zeigler, J., Liljeroth, E., Andreasson, E. Positive role of Salicylic acid COI1 signaling in early response to Alternaria solani in Potato (Manuscript)
VIBurra, D.D., Lenman, M, Levander, F, Resjo, S., Andreasson, E, Comparative proteomics of three different immunity reactions in Potato (Mansucript)
Place of Publication:Alnarp
Publisher:Department of Plant Protection Biology, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-8516-2
ISBN for electronic version:978-91-576-8517-9
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:H Protection of plants and stored products > H01 Protection of plants - General aspects
H Protection of plants and stored products > H20 Plant diseases
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding
Agrovoc terms:solanum tuberosum, phytophthora, plant diseases, pathogens, disease resistance, transcription, butyric acid, transfer rna, alternaria
Keywords:Phosphite, Phytophthora, Transcriptomics, Proteomics, Alternaria, Dickeya, SA, JA, PTI, ETI
Permanent URL:
ID Code:12945
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Protection Biology
External funders:Swedish Foundation for Strategic Research
Deposited On:13 Jan 2016 12:18
Metadata Last Modified:10 Sep 2020 13:41

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