Balkenius, Anna and Johansson, Anders J. and Balkenius, Christian
(2015).
Comparing analysis methods in functional calcium imaging of the insect brain.
PloS one. 10
:6
, 1-19
[Research article]
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Available under License Creative Commons Attribution. 2MB |
Official URL: http://dx.doi.org/10.1371/journal.pone.0129614
Abstract
We investigate four different methods for background estimation in calcium imaging of the insect brain and evaluate their performance on six data sets consisting of data recorded from two sites in two species of moths. The calcium fluorescence decay curve outside the potential response is estimated using either a low-pass filter or constant, linear or polynomial regression, and is subsequently used to calculate the magnitude, latency and duration of the response. The magnitude and variance of the responses that are obtained by the different methods are compared, and, by computing the receiver operating characteristics of a classifier based on response magnitude, we evaluate the ability of each method to detect the stimulus type and conclude that a polynomial approximation of the background gives the overall best result.
Authors/Creators: | Balkenius, Anna and Johansson, Anders J. and Balkenius, Christian | ||||||
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Title: | Comparing analysis methods in functional calcium imaging of the insect brain | ||||||
Series Name/Journal: | PloS one | ||||||
Year of publishing : | 2015 | ||||||
Depositing date: | 20 January 2016 | ||||||
Volume: | 10 | ||||||
Number: | 6 | ||||||
Page range: | 1-19 | ||||||
Number of Pages: | 19 | ||||||
Publisher: | Public Library of Science | ||||||
ISSN: | 1932-6203 | ||||||
Language: | English | ||||||
Publication Type: | Research article | ||||||
Refereed: | Yes | ||||||
Article category: | Scientific peer reviewed | ||||||
Version: | Published version | ||||||
Copyright: | Creative Commons: Attribution 4.0 | ||||||
Full Text Status: | Public | ||||||
Agris subject categories.: | X Agricola extesions > X30 Life sciences | ||||||
Subjects: | (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Zoology | ||||||
Agrovoc terms: | image analysis, brain, Insecta, calcium | ||||||
Keywords: | insect brain, calcium imaging | ||||||
URN:NBN: | urn:nbn:se:slu:epsilon-e-3182 | ||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3182 | ||||||
Additional ID: |
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ID Code: | 12967 | ||||||
Faculty: | LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap | ||||||
Department: | (LTJ, LTV) > Department of Plant Protection Biology | ||||||
Deposited By: | SLUpub Connector | ||||||
Deposited On: | 20 Jan 2016 15:28 | ||||||
Metadata Last Modified: | 09 Sep 2020 14:17 |
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