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The Apis mellifera Filamentous Virus Genome

Gauthier, Laurent and Cornman, Robert S and Hartmann, Ulrike and Cousserans, François and Evans, Jay D and Rodrigues De Miranda, Joachim and Neumann, Peter (2015). The Apis mellifera Filamentous Virus Genome. Viruses. 7:7, 3798-3815
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Abstract

A complete reference genome of the Apis mellifera Filamentous virus (AmFV) was determined using Illumina Hiseq sequencing. The AmFV genome is a double stranded DNA molecule of approximately 498,500 nucleotides with a GC content of 50.8%. It encompasses 247 non-overlapping open reading frames (ORFs), equally distributed on both strands, which cover 65% of the genome. While most of the ORFs lacked threshold sequence alignments to reference protein databases, twenty-eight were found to display significant homologies with proteins present in other large double stranded DNA viruses. Remarkably, 13 ORFs had strong similarity with typical baculovirus domains such as PIFs (per os infectivity factor genes: pif-1, pif-2, pif-3 and p74) and BRO (Baculovirus Repeated Open Reading Frame). The putative AmFV DNA polymerase is of type B, but is only distantly related to those of the baculoviruses. The ORFs encoding proteins involved in nucleotide metabolism had the highest percent identity to viral proteins in GenBank. Other notable features include the presence of several collagen-like, chitin-binding, kinesin and pacifastin domains. Due to the large size of the AmFV genome and the inconsistent affiliation with other large double stranded DNA virus families infecting invertebrates, AmFV may belong to a new virus family.

Authors/Creators:Gauthier, Laurent and Cornman, Robert S and Hartmann, Ulrike and Cousserans, François and Evans, Jay D and Rodrigues De Miranda, Joachim and Neumann, Peter
Title:The Apis mellifera Filamentous Virus Genome
Series/Journal:Viruses (1999-4915)
Year of publishing :2015
Depositing date:25 January 2015
Volume:7
Number:7
Page range:3798-3815
Number of Pages:18
Publisher:MDPI
ISSN:1999-4915
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:L Animal production > L10 Animal genetics and breeding
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Pathobiology
Agrovoc terms:Apis mellifera, viruses, genomes
Keywords:Apis mellifera, filamentous virus, genome annotation
URN:NBN:urn:nbn:se:slu:epsilon-e-3187
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3187
Additional ID:
Type of IDID
Web of Science (WoS)000360353200024
DOI10.3390/v7072798
ID Code:12981
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Ecology
(S) > Dept. of Ecology
External funders:L'Agence Nationale de la Recherche / ANR
Deposited By: SLUpub Connector
Deposited On:25 Jan 2016 14:34
Metadata Last Modified:10 Feb 2016 14:24

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