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Malpighian tubules are important determinants of Pseudomonas transstadial transmission and longtime persistence in Anopheles stephensi

Chavshin, Ali Reza and Oshaghi, Mohammad Ali and Vatandoost, Hasan and Yakhchali, Bagher and Zarenejad, Fahimeh and Terenius, Olle (2015). Malpighian tubules are important determinants of Pseudomonas transstadial transmission and longtime persistence in Anopheles stephensi. Parasites & Vectors. 8, 1-7
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Official URL: http://dx.doi.org/10.1186/s13071-015-0635-6

Abstract

Background: Pseudomonas is a genus of bacteria commonly found in investigations of gut microbes in malaria mosquitoes. Among those mosquitoes is the dominating malaria vector in Asia, Anopheles stephensi, where Pseudomonas is a prevailing bacterium and natural inhabitant of its breeding places. In order to explore the reason for finding Pseudomonas so frequently, an investigation of its localization and transstadial properties was undertaken.Methods: A Pseudomonas isolate from Anopheles stephensi was transformed successfully with an endogenous plasmid modified to express green fluorescent protein (GFP). Subsequently, the Pseudomonas-GFP was added to the laboratory larval breeding place of Anopheles stephensi and taken up by the larvae. After 24 hours, the larvae were cleaned and moved to a bath with double-distilled water. Also, female adults were fed sugar solution containing Pseudomonas-GFP. The Pseudomonas-GFP was traced in the alimentary canal of larvae, pupae and adults.Results: Fluorescent microscopy and PCR assays showed that the Pseudomonas bacteria underwent transstadial transmission from larvae to pupae and then to adults. In blood-fed female mosquitoes, the bacteria increased in numbers and remained in the mosquito body for at least three weeks after eclosion. In addition to the midgut, the Malpighian tubules of both larvae and adult mosquitoes were colonized by the bacteria. Also Pseudomonas-GFP that was distributed through sugar solution was able to colonize the Malpighian tubules of adult females.Conclusions: Colonization of the Malpighian tubules by Pseudomonas bacteria seems to be important for the transstadial passage from larvae to adult and presumably for the longevity of the bacteria in the adult mosquito. The existence of an entry point in the larval stage, and the long duration in the female gut, opens up for a possible use of Pseudomonas in mosquito paratransgenesis.

Authors/Creators:Chavshin, Ali Reza and Oshaghi, Mohammad Ali and Vatandoost, Hasan and Yakhchali, Bagher and Zarenejad, Fahimeh and Terenius, Olle
Title:Malpighian tubules are important determinants of Pseudomonas transstadial transmission and longtime persistence in Anopheles stephensi
Series/Journal:Parasites & Vectors (1756-3305)
Year of publishing :2015
Depositing date:15 February 2016
Volume:8
Page range:1-7
Number of Pages:7
Publisher:BioMed Central
ISSN:1756-3305
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:X Agricola extesions > X30 Life sciences
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
Agrovoc terms:malaria, anopheles stephensi, pseudomonas, fluorescence, proteins
Keywords:Malaria, Anopheles stephensi, Pseudomonas, GFP, Transstadial transmission, Paratransgenesis
URN:NBN:urn:nbn:se:slu:epsilon-e-3216
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3216
Additional ID:
Type of IDID
Web of Science (WoS)000349417300001
DOI10.1186/s13071-015-0635-6
ID Code:13046
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Ecology
(S) > Dept. of Ecology
Deposited By: SLUpub Connector
Deposited On:15 Feb 2016 14:53
Metadata Last Modified:25 Feb 2016 15:05

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