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Developmental succession of the microbiome of Culex mosquitoes

Duguma, Dagne and Hall, Michael W. and Rugman-Jones, Paul and Stouthamer, Richard and Tereniu, Olle and Neufeld, Josh D. and Walton, William E. (2015). Developmental succession of the microbiome of Culex mosquitoes. BMC Microbiology. 15, 1-13
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Official URL: http://dx.doi.org/10.1186/s12866-015-0475-8

Abstract

Background: The native microflora associated with mosquitoes have important roles in mosquito development and vector competence. Sequencing of bacterial V3 region from 16S rRNA genes across the developmental stages of Culex mosquitoes (early and late larval instars, pupae and adults) was used to test the hypothesis that bacteria found in the larval stage of Culex are transstadially transmitted to the adult stage, and to compare the microbiomes of field-collected versus laboratory-reared mosquitoes.Results: Beta diversity analysis revealed that bacterial community structure differed among three life stages (larvae, pupae and adults) of Culex tarsalis. Although only similar to 2 % of the total number of bacterial OTUs were found in all stages, sequences from these OTUs accounted for nearly 82 % of the total bacterial sequences recovered from all stages. Thorsellia (Gammaproteobacteria) was the most abundant bacterial taxon found across all developmental stages of field-collected Culex mosquitoes, but was rare in mosquitoes from laboratory-reared colonies. The proportion of Thorsellia sequences in the microbiomes of mosquito life stages varied ontogenetically with the greatest proportions recovered from the pupae of C. tarsalis and the lowest from newly emerged adults. The microbiome of field-collected late instar larvae was not influenced significantly by differences in the microbiota of the habitat due to habitat age or biopesticide treatments. The microbiome diversity was the greatest in the early instar larvae and the lowest in laboratory-reared mosquitoes.Conclusions: Bacterial communities in early instar C. tarsalis larvae were significantly more diverse when compared to late instar larvae, pupae and newly emerged adults. Some of the bacterial OTUs found in the early instar larvae were also found across developmental stages. Thorsellia dominated the bacterial communities in field-collected immature stages but occurred at much lower relative abundance in adults. Differences in microbiota observed in larval habitats did not influence bacterial community profiles of late instar larvae or adults. However, bacterial communities in laboratory-reared C. tarsalis larvae differed significantly from the field. Determining the role of Thorsellia in mosquitoes and its distribution across different species of mosquitoes warrants further investigation.

Authors/Creators:Duguma, Dagne and Hall, Michael W. and Rugman-Jones, Paul and Stouthamer, Richard and Tereniu, Olle and Neufeld, Josh D. and Walton, William E.
Title:Developmental succession of the microbiome of Culex mosquitoes
Series/Journal:BMC Microbiology (1471-2180)
Year of publishing :2015
Depositing date:19 February 2016
Volume:15
Page range:1-13
Number of Pages:13
Publisher:BioMed Central Ltd
ISSN:1471-2180
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:X Agricola extesions > X30 Life sciences
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
Agrovoc terms:bacteria, biopesticides, vectorborne diseases, intestinal flora, culicidae
Keywords:Thorsellia, Outdoor mesocosms, Bacteria, Biopesticide, Transstadial transmission
URN:NBN:urn:nbn:se:slu:epsilon-e-3234
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3234
Additional ID:
Type of IDID
Web of Science (WoS)000358346800001
DOI10.1186/s12866-015-0475-8
ID Code:13071
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Ecology
(S) > Dept. of Ecology
Deposited By: SLUpub Connector
Deposited On:19 Feb 2016 14:45
Metadata Last Modified:02 Mar 2016 03:28

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