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Immunopathological aspects of equine inflammatory bowel disease

Olofsson, Karin (2016). Immunopathological aspects of equine inflammatory bowel disease. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2016:11
ISBN 978-91-576-8524-7
eISBN 978-91-576-8525-4
[Doctoral thesis]

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Abstract

Intestinal inflammation is a major welfare issue for horses causing colic, diarrhoea and an inability to thrive. One of the diagnoses with poor prognosis is inflammatory bowel disease (eqIBD). Equine IBD comprises different entities of chronic idiopathic enteropathies, namely eosinophilic gastroenteritis, granulomatous enteritis and lymphoplasmacytic enterocolitis. An exaggerated immune reactivity towards still unidentified antigens is suggested in the disease. The aim of this thesis was therefore to elaborate methods to characterize cellular infiltrations and to determine cytokine profiles associated with various forms of eqIBD. Jejunal tissues were used to describe the immune cell populations in situ in healthy horses and in well characterised IBD-afflicted horses. T cells, regulatory T cells, B cells, IgM- IgG- and IgA-secreting plasma cells and MHC II-expressing cells were labelled by immunohistochemistry and quantified by image analysis. The inflammation in IBD horses was dominated by T cells and MHC II-expressing cells, whereas B cells and plasma cells were decreased compared with healthy horses. These features were most prominent in granulomatous IBD. The regulatory T cells followed the infiltration pattern of T cells, but were not significantly increased in diseased horses. Thus the histopathology in the studied forms of eqIBD shows similarities with a delayed hypersensitivity reaction. Seven reference genes were evaluated and their optimal combination determined for seven equine intestinal segments (the duodenum, mid-jejunum, ileum, caecum, right ventral and dorsal colon and rectum). Segments from healthy horses expressed IL-12, IL-17A, IL-23, TLR4, but not IFN-α. The relative expression of the three interleukins and TLR4 was analysed using quantitative PCR in rectal biopsies showing chronic or chronic active simple proctitis from horses with clinical signs of eqIBD. Horses with chronic active proctitis had increased expression of IL-17A and TLR4 compared with healthy horses, whereas horses with chronic proctitis had decreased expression of IL-12, indicating a TH17 involvement in chronic active disease. Taken together, techniques were established to discern patterns of immune reactions and could demonstrate differences between entities when applied to eqIBD material.

Authors/Creators:Olofsson, Karin
Title:Immunopathological aspects of equine inflammatory bowel disease
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :2016
Depositing date:23 February 2016
Volume:2016:11
Number of Pages:95
Papers/manuscripts:
NumberReferences
IOlofsson, K.M., Press, C.M., Fossum, C. & Lindberg, R. Immunohistochemical characterization of immune cells in the jejunum of healthy horses and horses with inflammatory bowel disease. In manuscript.
IIFossum, C., Hjertner, B., Olofsson, K.M., Lindberg, R., Ahooghalandari, P., Camargo, M.M., Bröjer, J., Edner, A. & Nostell, K. (2012). Expression of tlr4, md2 and cd14 in equine blood leukocytes during endotoxin infusion and in intestinal tissues from healthy horses. Veterinary Immunology and Immunopathology, 150(3-4), pp. 141-148.
IIIHjertner, B., Olofsson, K.M., Lindberg, R., Fuxler, L. & Fossum, C. (2013). Expression of reference genes and T helper 17 associated cytokine genes in the equine intestinal tract. The Veterinary Journal, 197(3), pp. 817-823.
IVOlofsson, K.M., Hjertner, B., Fossum, C., Press, C.M. & Lindberg, R. (2015). Expression of Th17-associated cytokines and toll-like receptor 4 and their correlation to Foxp3 positive cells in rectal biopsies of horses with clinical signs of inflammatory bowel disease. The Veterinary Journal, 206(1), pp. 97-104
Place of Publication:Uppsala
Publisher:Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-8524-7
ISBN for electronic version:978-91-576-8525-4
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:L Animal production > L73 Animal diseases
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Pathobiology
Agrovoc terms:horses, intestines, intestinal diseases, animal diseases, inflammation, cytokines, gene expression, symptoms, diagnosis, immunological techniques
Keywords:equine, intestine, immunopathology, chronic idiopathic inflammatory enteropathy, inflammatory bowel disease
URN:NBN:urn:nbn:se:slu:epsilon-e-3242
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3242
ID Code:13093
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
External funders:Stiftelsen Hästforskning
Deposited By: Karin Olofsson
Deposited On:23 Feb 2016 09:57
Metadata Last Modified:05 Mar 2016 15:03

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