Home About Browse Search
Svenska


Genotype analysis and studies of pyrethroid resistance of the oilseed rape (Brassica napus) insect pest - pollen beetle (Meligethes aeneus)

Kazachkova, Nadiya (2007). Genotype analysis and studies of pyrethroid resistance of the oilseed rape (Brassica napus) insect pest - pollen beetle (Meligethes aeneus). Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2007:11
ISBN 978-91-576-7310-7
[Doctoral thesis]

[img]
Preview
PDF
1MB
[img]
Preview
["document_typename_coverimage" not defined]
26kB

Abstract

Oilseed Brassicas are vulnerable to attack from many insects and pathogens, calling for an extensive use of pesticides to secure crop yields; this can cause increased resistance in pests. During recent years, one of the main oilseed insect pests—the pollen beetle (Meligethes aeneus), resistant to pyrethroid insecticides—has emerged in southern Sweden. This, because of its frequency and geographic range, provides an excellent source of material for analysis of genetic variation among pollen beetle populations, for study of insecticide resistance and for testing new sources of plant protection. For genotyping pollen beetles, we modified the amplified fragment length polymorphism (AFLP) technique (chosen because it does not depend on prior sequence information when no genome information is available for pollen beetles), and applied it to 133 Swedish populations (susceptible and resistant), collected in different years, and to 14 European populations. AMOVA showed high levels of genetic variation within populations and gene flow among populations, and no evidence of expected regional and resistance-susceptibility to insecticide diversification (clear diversification by time and generations instead) for Swedish populations. European populations showed a clear pattern of regional diversification and a low level of gene flow. To identify possible point mutations associated with pollen beetles resistance to pyrethroids, the primary target sites for pyrethroids—voltage-sensitive sodium channels (VSSC) and metabolic resistance sites—Cytochrome P450, were studied using RT-PCR in resistant and susceptible insects. Two CYP450 partial cDNAs and four cDNA fragments composing VSSC domains I and II were amplified (using primers designed for homologue sequences) and sequenced showing point mutations, which can confer pyrethroid resistance.

Authors/Creators:Kazachkova, Nadiya
Title:Genotype analysis and studies of pyrethroid resistance of the oilseed rape (Brassica napus) insect pest - pollen beetle (Meligethes aeneus)
Year of publishing :2007
Volume:2007:11
Number of Pages:56
Papers/manuscripts:
NumberReferences
ALLI. Kazachkova, N., Fahleson, J., & Meijer, J. (2004) Establishment of the Amplified Fragment Length Polymorphism (AFLP) technique for genotyping of pollen beetle (Meligethes aeneus) - a noxious insect pest on oilseed rape (Brassica napus). Molecular Biology Reports. 31: 37-42. II. Kazachkova, N., Meijer, J. & Ekbom, B. (2006). Genetic diversity in pollen beetles (Meligethes aeneus) in Sweden: role of spatial, temporal and insecticide resistance factors. (Submitted). III. Kazachkova, N., Meijer, J. & Ekbom, B. (2006). Genetic diversity in European pollen beetle (Meligethes aeneus) populations. (Submitted). IV. Kazachkova, N., Meijer, J. & Ekbom, B. (2006). Analysis of common insecticide resistance gene (Voltage sensitive sodium channel gene and CYP450 genes) in pollen beetles (Meligethes aeneus): point mutations associated with resistance to pyrethroids. (Manuscript).
Place of Publication:Uppsala
ISBN for printed version:978-91-576-7310-7
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:meligethes aeneus, pest insects, genotypes, genetic variation, genetic markers, synthetic pyrethrins, insecticides, pesticide resistance, brassica napus, oil crops, insect control
Keywords:AFLP, genotyping, insect pest, genetic variation, insecticide resistance, VSSC, pyrethroid, CYP450.
URN:NBN:urn:nbn:se:slu:epsilon-1425
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-1425
ID Code:1329
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: Nadiya Kazachkova
Deposited On:29 Jan 2007 00:00
Metadata Last Modified:02 Dec 2014 10:11

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits