Kazachkova, Nadiya
(2007).
Genotype analysis and studies of pyrethroid resistance of the oilseed rape (Brassica napus) insect pest - pollen beetle (Meligethes aeneus).
Diss. (sammanfattning/summary)
Uppsala :
Sveriges lantbruksuniv.,
Acta Universitatis Agriculturae Sueciae, 1652-6880
; 2007:11
ISBN 978-91-576-7310-7
[Doctoral thesis]
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Abstract
Oilseed Brassicas are vulnerable to attack from many insects and pathogens, calling for an extensive use of pesticides to secure crop yields; this can cause increased resistance in pests. During recent years, one of the main oilseed insect pests—the pollen beetle (Meligethes aeneus), resistant to pyrethroid insecticides—has emerged in southern Sweden. This, because of its frequency and geographic range, provides an excellent source of material for analysis of genetic variation among pollen beetle populations, for study of insecticide resistance and for testing new sources of plant protection. For genotyping pollen beetles, we modified the amplified fragment length polymorphism (AFLP) technique (chosen because it does not depend on prior sequence information when no genome information is available for pollen beetles), and applied it to 133 Swedish populations (susceptible and resistant), collected in different years, and to 14 European populations. AMOVA showed high levels of genetic variation within populations and gene flow among populations, and no evidence of expected regional and resistance-susceptibility to insecticide diversification (clear diversification by time and generations instead) for Swedish populations. European populations showed a clear pattern of regional diversification and a low level of gene flow. To identify possible point mutations associated with pollen beetles resistance to pyrethroids, the primary target sites for pyrethroids—voltage-sensitive sodium channels (VSSC) and metabolic resistance sites—Cytochrome P450, were studied using RT-PCR in resistant and susceptible insects. Two CYP450 partial cDNAs and four cDNA fragments composing VSSC domains I and II were amplified (using primers designed for homologue sequences) and sequenced showing point mutations, which can confer pyrethroid resistance.
Authors/Creators: | Kazachkova, Nadiya | ||||
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Title: | Genotype analysis and studies of pyrethroid resistance of the oilseed rape (Brassica napus) insect pest - pollen beetle (Meligethes aeneus) | ||||
Year of publishing : | 2007 | ||||
Number: | 2007:11 | ||||
Number of Pages: | 56 | ||||
Papers/manuscripts: |
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Place of Publication: | Uppsala | ||||
ISBN for printed version: | 978-91-576-7310-7 | ||||
ISSN: | 1652-6880 | ||||
Language: | English | ||||
Publication Type: | Doctoral thesis | ||||
Full Text Status: | Public | ||||
Agrovoc terms: | meligethes aeneus, pest insects, genotypes, genetic variation, genetic markers, synthetic pyrethrins, insecticides, pesticide resistance, brassica napus, oil crops, insect control | ||||
Keywords: | AFLP, genotyping, insect pest, genetic variation, insecticide resistance, VSSC, pyrethroid, CYP450. | ||||
URN:NBN: | urn:nbn:se:slu:epsilon-1425 | ||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-1425 | ||||
ID Code: | 1329 | ||||
Department: | (NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231) | ||||
Deposited By: | Nadiya Kazachkova | ||||
Deposited On: | 29 Jan 2007 00:00 | ||||
Metadata Last Modified: | 02 Dec 2014 10:11 |
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