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Anatomical and Immunocoverage Observations on SuSy, C4H, and Pectate Lyase Family Protein Down-regulated Aspens Genotypes

Sandquist, David and Norell, Lennart and Takabe, Keiji and Yoshinaga, Arata and Daniel, Geoffrey (2015). Anatomical and Immunocoverage Observations on SuSy, C4H, and Pectate Lyase Family Protein Down-regulated Aspens Genotypes. Bioresources. 10:3, 5016-5029
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Abstract

This study aims to complement previous studies on down-regulation of sucrose synthase and cinnamate 4-hydroxylase in hybrid aspen (Bjurhager et al. 2010; Gerber et al. 2014) with a quantitative anatomical comparison (Sandquist et al. 2015). The main focus was placed on evaluating quantitative modifications to fiber morphology and lignin composition. This was achieved by combining results from light-, electron-, and UV-microscopy together with histochemical staining. Overall there was good agreement between the morphological results in this study compared to the previously published chemical and physical reports, particularly on cinnamate 4-hydroxylase down-regulated aspen. The previously reported statistical correlations between fiber lumen area and plant diameter, and fiber length and tree height (Sandquist et al. 2015) have been further refined. Knock-down of genes coding for biologically important proteins such as sucrose synthase and cinnamate 4-hydroxylase had broad spanning morphological impacts. Down-regulated sucrose synthase genotype showed significantly reduced fiber development, possibly with altered S/G ratio. Down-regulated cinnamate 4-hydroxylase genotype showed an overall reduction of lignin in fibers as indicated from both chemical staining and UV-microscopy measurements. Down-regulated pectate lyase genotype showed no overall significant effects on fiber morphology, ultrastructural, nor lignin staining.

Authors/Creators:Sandquist, David and Norell, Lennart and Takabe, Keiji and Yoshinaga, Arata and Daniel, Geoffrey
Title:Anatomical and Immunocoverage Observations on SuSy, C4H, and Pectate Lyase Family Protein Down-regulated Aspens Genotypes
Series/Journal:Bioresources (1930-2126)
Year of publishing :2015
Volume:10
Number:3
Page range:5016-5029
Number of Pages:14
Publisher:North Carolina State University. Dept. of Wood and Paper Science
ISSN:1930-2126
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Wood Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Forest Science
Agrovoc terms:forestry
Keywords:transgenic poplar, wood ultrastructure, cinnamate 4-hydroxylase (KR139), sucrose synthase (KR112), pectate lyase family protein (KR213), UV-microscopy, lignin staining, quantitative wood anatomy
Additional ID:
Type of IDID
Web of Science (WoS)000364199800094
ID Code:13295
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
S - Faculty of Forest Sciences
Department:(NL, NJ) > Dept. of Economics
(S) > Dept. of Forest Products
Deposited By: SLUpub Connector
Deposited On:25 Apr 2016 08:49
Metadata Last Modified:25 Apr 2016 08:49

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