with special emphasis on somatic embryogenesis in Scots pine (Pinus sylvestris L.)
Abrahamsson, Malin
(2016).
Patterning during embryo development in Pinus.
Diss. (sammanfattning/summary)
Uppsala :
Sveriges lantbruksuniv.,
Acta Universitatis Agriculturae Sueciae, 1652-6880
; 2016:48
ISBN 978-91-576-8598-8
eISBN 978-91-576-8599-5
[Doctoral thesis]
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Abstract
Somatic embryogenesis is an attractive method to propagate conifers vegetatively. However, many species belonging to Pinus are recalcitrant to somatic embryo development. The overall aim of this thesis has been to gain a better understanding of the developmental pathway leading to cotyledonary somatic embryos of Scots pine (Pinus sylvestris).
By comparing the developmental pathway of somatic embryos in a normal and in an abnormal cell line, differences between the cell lines were identified. An important difference was the high ratio of early and late embryos carrying supernumerary suspensor cells in the abnormal cell line compared to embryos in the normal cell line. This unbalanced ratio between the embryonal mass and the suspensor is at least partly caused by a disturbed polar auxin transport. Furthermore, a high proportion of early and late embryos degenerated in both cell lines. The degenerating embryos in the normal cell line were eliminated in a similar way as subordinate embryos in the seed. Contrastingly, the degenerating embryos in the abnormal cell line were not eliminated; instead the degenerated embryos started to differentiate new embryos creating a loop of embryo degeneration and embryo differentiation. During initiation of embryogenic tissue, the protruding early zygotic embryo(s) started to degenerate before a proliferating embryogenic culture was established, indicating that the initiation of embryogenic cultures is not a direct continuation of cleavage polyembryony. The results further suggest that there is a high risk that cell lines initiated from early zygotic embryos at the stage of cleavage develop abnormally.
To be able to use more differentiated tissues as explants for initiation of embryogenic cultures, more knowledge is needed about totipotence and embryogenic potential. Treatment with the histone deacetylase inhibitor trichostatin A (TSA) during maturation of somatic embryos of Norway spruce inhibited the maturation progression of the embryos. Furthermore, although TSA-treatment maintained the embryogenic potential in germinating somatic embryos, it did not enable the embryos to regain embryogenic potential after it was lost.
By analysing global changes in gene expression during early zygotic embryo development in Scots pine, we identified genes and processes that might be important for regulating the cleavage process and for the development of a dominant embryo.
Together these results contribute to the knowledge that in turn can lead to improved protocols for large scale propagation of Pinus species via somatic embryos.
Authors/Creators: | Abrahamsson, Malin | ||||||||||
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Title: | Patterning during embryo development in Pinus | ||||||||||
Subtitle: | with special emphasis on somatic embryogenesis in Scots pine (Pinus sylvestris L.) | ||||||||||
Alternative abstract: |
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Series/Journal: | Acta Universitatis Agriculturae Sueciae (1652-6880) | ||||||||||
Year of publishing : | 2016 | ||||||||||
Depositing date: | 22 April 2016 | ||||||||||
Number: | 2016:48 | ||||||||||
Number of Pages: | 58 | ||||||||||
Papers/manuscripts: |
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Place of Publication: | Uppsala | ||||||||||
Publisher: | Department of Plant Biology, Swedish University of Agricultural Sciences | ||||||||||
ISBN for printed version: | 978-91-576-8598-8 | ||||||||||
ISBN for electronic version: | 978-91-576-8599-5 | ||||||||||
ISSN: | 1652-6880 | ||||||||||
Language: | English | ||||||||||
Publication Type: | Doctoral thesis | ||||||||||
Full Text Status: | Public | ||||||||||
Agris subject categories.: | F Plant production > F30 Plant genetics and breeding F Plant production > F62 Plant physiology - Growth and development K Forestry > K01 Forestry - General aspects | ||||||||||
Subjects: | (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Cell Biology (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Developmental Biology (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Forest Science | ||||||||||
Agrovoc terms: | pinus sylvestris, pinophyta, somatic embryos, somatic embryogenesis, auxins, apoptosis, transcription, gene expression | ||||||||||
Keywords: | Conifer, Embryogenic potential, Polar auxin transport, Programmed cell death, Scots pine, somatic embryos, somatic embryogenesis, transcriptome | ||||||||||
URN:NBN: | urn:nbn:se:slu:epsilon-e-3399 | ||||||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3399 | ||||||||||
ID Code: | 13298 | ||||||||||
Faculty: | NJ - Fakulteten för naturresurser och jordbruksvetenskap | ||||||||||
Department: | (NL, NJ) > Department of Plant Biology (from 140101) | ||||||||||
Deposited By: | Malin Abrahamsson | ||||||||||
Deposited On: | 25 Apr 2016 11:13 | ||||||||||
Metadata Last Modified: | 10 Sep 2020 13:41 |
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