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Developmentally regulated non-coding RNAs in the social amoeba Dictyostelium discoideum

Hinas, Andrea (2007). Developmentally regulated non-coding RNAs in the social amoeba Dictyostelium discoideum. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 ; 2007:8
ISBN 978-91-576-7307-7
[Doctoral thesis]

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Non-coding RNAs (ncRNAs) are RNAs that do not encode proteins but instead carry out their functions at the RNA level. During the past decade, ncRNAs have been recognized as major regulators in organisms representing all kingdoms of life. Despite their recent discovery, these ncRNAs are involved in vital processes ranging from cell differentiation and organism development to virulence and stress responses. To exert these important functions, ncRNAs affect e.g. chromatin modification, RNA destabilization, inhibition of translation, RNA modification, and protein translocation. Their sizes are as diverse as their functions, varying from ~20 nucleotides up to several thousands of nucleotides. The social amoeba Dictyostelium discoideum has for many years been an appreciated model system for e.g. organism development, infection, and chemotaxis, but its ncRNAs have been largely unexplored. In this thesis, experimental as well as computational approaches were employed to investigate the ncRNA profile of this genetically tractable model organism. The identified RNAs include RNA classes with homologs in other organisms, such as small nucleolar RNAs, spliceosomal RNAs, and small interfering RNAs, as well as a large number of entirely novel RNAs. Many of the D. discoideum ncRNAs are developmentally regulated, indicating a function in organism development. Although many ncRNAs are now known to play crucial roles in biology, the functions of most ncRNAs are yet to be discovered. In this quest, easily manipulated model organisms will be invaluable. This thesis further establishes D. discoideum as an attractive model system for discerning ncRNA function and regulation.

Authors/Creators:Hinas, Andrea
Title:Developmentally regulated non-coding RNAs in the social amoeba Dictyostelium discoideum
Series Name/Journal:Acta Universitatis Agriculturae Sueciae
Year of publishing :2007
Number of Pages:55
ALLI. *Aspegren, A., *Hinas, A., Larsson, P., Larsson, A., & Söderbom, F. 2004. Novel non-coding RNAs in Dictyostelium discoideum and their expression during development. Nucleic Acids Research 32, 4646-4656. II. *Hinas, A., *Larsson, P., Avesson, L., Kirsebom, L. A., Virtanen, A., & Söderbom, F. 2006. Identification of the major spliceosomal RNAs in Dictyostelium discoideum reveals developmentally regulated U2 variants and polyadenylated snRNAs. Eukaryotic Cell 5, 924-934. III. Larsson, P., Hinas, A., Ardell, D., Kirsebom, L., Virtanen, A., & Söderbom, F. Application of Karlin-Dembo statistics concerning maximal scoring Markov-dependent subsequences to find candidate non-coding RNAs in the Dictyostelium discoideum genome (Manuscript). IV. Hinas A., Reimegård, J, Wagner E.G.H., Nellen W., Ambros, V., & Söderbom F. The small RNA repertoire of Dictyostelium discoideum: small antisense RNAs that may be derived from longer transcripts and multiple classes of repeat-associated RNAs. (Manuscript). *Shared first authorship
Place of Publication:Uppsala
ISBN for printed version:978-91-576-7307-7
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:dictyostelium discoideum, rna, identification, plant models
Keywords:Dictyostelium discoideum, non-coding RNA, small nucleolar RNA, spliceosomal RNA, RNA interference, antisense RNA, development
Permanent URL:
ID Code:1332
Department:(NL, NJ) > Dept. of Molecular Biology (until 131231)
Deposited By: Andrea Hinas
Deposited On:25 Jan 2007 00:00
Metadata Last Modified:02 Dec 2014 10:11

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