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In Vitro Regeneration of Endangered Medicinal Hypoxis Species

Nsibande, Busisiwe and Li, Xue-Yuan and Ahlman, Annelie and Zhu, Li-Hua (2015). In Vitro Regeneration of Endangered Medicinal Hypoxis Species. American Journal of Plant Sciences. 6:16, 2585-2595
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Abstract

The genus Hypoxis contains some of southern Africa's most important wild medicinal species, a situation that has placed most of them amongst endangered species facing extinction. In this study, four Hypoxis species collected from Swaziland: H. argentea, H. filiformis, H. acuminata and H. hemerocallidea, were included to assess their potential for in vitro propagation in order to efficiently conserve these species in the future. Among all types of explants tested only corm explants and seeds gave rise to shoots under the in vitro conditions. For the corm establishment, H. filiformis was the most in vitro responsive species, which had 100% shoot regeneration with high shoot number when cultured on the MS medium supplemented with 3 mg·l-1 kinetin with a piece of shoot attached, while the highest mean shoot number (17) was obtained on the same medium, but with no shoot attachment to the corm explants. H. argentea was the next most responsive species in vitro with up to 70% establishment, while the other two species had only up to 20% of in vitro establishment. For the seed explants, up to 29% seed germination was obtained for H. argentea when the seed coats were crushed before the in vitro culture. The regenerated shoots were rooted in vitro and acclimatized in the greenhouse successfully

Authors/Creators:Nsibande, Busisiwe and Li, Xue-Yuan and Ahlman, Annelie and Zhu, Li-Hua
Title:In Vitro Regeneration of Endangered Medicinal Hypoxis Species
Series/Journal:American Journal of Plant Sciences (2158-2742)
Year of publishing :2015
Volume:6
Number:16
Page range:2585-2595
Number of Pages:11
Publisher:Scientific Research Publishing
Associated Programs and Other Stakeholders:SLU - Environmental assessment > Programme Agricultural landscape
SLU - Research Areas for the Future > Future Agriculture
ISSN:2158-2742
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:F Plant production > F02 Plant propagation
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Agrovoc terms:plant production, biological preservation
Keywords:Corm, Seed, Micropropagation, Plant Growth Regulators, Hypoxis
URN:NBN:urn:nbn:se:slu:epsilon-e-3459
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3459
Additional ID:
Type of IDID
DOI10.4236/ajps.2015.616261
ID Code:13344
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:26 May 2016 09:47
Metadata Last Modified:26 May 2016 11:02

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