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Quantitative evaluation of hybrid aspen xylem and immunolabeling patterns using image analysis and multivariate statistics

Sandquist, David and Norell, Lennart and Daniel, Geoffrey (2015). Quantitative evaluation of hybrid aspen xylem and immunolabeling patterns using image analysis and multivariate statistics. Bioresources. 10:3, 4997-5015
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Abstract

In this study we present a method for quantitative evaluation of hybrid aspen genotypes xylem morphology and immunolabeling microdistribution. The method is based on image analysis and multivariate statistical evaluation of light and immunofluorescence microscopy images of wood xylem cross sections. The selected immunolabeling antibodies targeted five different epitopes present in the aspen xylem cell walls. Twelve down-regulated hybrid aspen genotypes have been included in the method development. The twelve knock-down genotypes were selected based on pre-screening by pyrolysis-IR of global chemical content. The multivariate statistical evaluations successfully identified comparative trends for modifications in the down-regulated genotypes compared to the unmodified control, even when no definitive conclusions could be drawn from individual studied variables alone. Of the twelve genotypes analysed, three genotypes showed significant trends for modification in both morphology and immunolabeling. Six genotypes showed significant trends for modification in either morphology or immunocoverage. The remaining three genotypes did not show any significant trends for modification.

Authors/Creators:Sandquist, David and Norell, Lennart and Daniel, Geoffrey
Title:Quantitative evaluation of hybrid aspen xylem and immunolabeling patterns using image analysis and multivariate statistics
Series/Journal:Bioresources (1930-2126)
Year of publishing :2015
Depositing date:2 June 2016
Volume:10
Number:3
Page range:4997-5015
Number of Pages:19
Publisher:North Carolina State University
ISSN:1930-2126
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:F Plant production > F50 Plant structure
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Wood Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Forest Science
Agrovoc terms:wood, genotypes, immunology, microscopy
Keywords:Wood genotyping, immunocoverage, wood chemistry, immunolabeling, quantitative microscopy
URN:NBN:urn:nbn:se:slu:epsilon-e-3470
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3470
Additional ID:
Type of IDID
Web of Science (WoS)000364199800093
DOI10.15376/biores.10.3.4997-5015
ID Code:13373
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Economics
Deposited By: SLUpub Connector
Deposited On:02 Jun 2016 13:41
Metadata Last Modified:02 Jun 2016 13:42

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