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Genetic, morphological and chemical variation in the genus Harpagophytum

Muzila, Mbaki (2016). Genetic, morphological and chemical variation in the genus Harpagophytum. Diss. (sammanfattning/summary) Alnarp : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2016:67
ISBN 978-91-576-8636-7
eISBN 978-91-576-8637-4
[Doctoral thesis]

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Abstract

Harpagophytum procumbens is a valuable medicinal plant but mistakes with plant identification at harvesting, and wilful adulteration using the presumably less efficient H. zeyheri are suggested. A multidisciplinary study with plant material from Botswana was conducted. (1) Morphometric measurements on seed capsules showed continuous variation between and, in some cases, within locations indicating occurrence of interspecific hybridization. (2) Mitotic and meiotic chromosome counts, that need to be ascertained, suggest 2n=20, 20/22 and 24 in the studied materials. Meiosis in a putative hybrid appeared regular but occurrence of dyads and triads at the tetrad stage indicated disturbances. (3) DNA markers for seedlings from field-collected seed capsules suggest that outcrossing is prevalent. While the two species showed significant differentiation, some morphologically deviating plants had intermediate DNA profiles possibly representing introgression products. (4) Analysis of selected biochemical compounds in peel and pulp of the tubers showed that content of the main bioactive compound harpagoside was significantly higher in H. procumbens compared to putative hybrids and H. zeyheri. In both peel and pulp, contents of isoverbascoside, verbascoside, acetylacteoside and pagoside were higher in H. procumbens and putative hybrids compared to H. zeyheri. (5) Tuber extracts were investigated for antioxidant capacity, content of total phenols, important phenylpropanoids and selected iridoids, and capacity to suppress respiratory burst in terms of reactive oxygen species produced by human neutrophils challenged with phorbol myristate acetate, opsonized Staphylococcus aureus and Fusobacterium nucleatum. A new variety of H. procumbens ssp. transvaalense showed the highest degree of antioxidative capacity, wheras an anti-inflammatory effect could not be corroborated for any of the tested extracts.

Authors/Creators:Muzila, Mbaki
Title:Genetic, morphological and chemical variation in the genus Harpagophytum
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :2016
Depositing date:14 May 2016
Volume:2016:67
Number of Pages:74
Papers/manuscripts:
NumberReferences
IMuzila, M., Werlemark, G., Ortiz, R., Sehic, J., Fatih, M., Setshogo, M., Mpoloka, W. and Nybom, H. (2014). Assessment of diversity in Harpagophytum with RAPD and ISSR markers provides evidence of introgression. Hereditas 151: 91–101.
IIMuzila M., Ekholm A., Nybom H., Widén C. and Rumpunen K. Harpagophytum germplasm varies in tuber peel and pulp content of important phenylpropanoids and iridoids. Manuscript submitted.
IIIMuzila, M., Rumpunen, K., Wright, H., Roberts, H., Grant, M., Nybom, H., Sehic, J., Ekholm, A. and Widén, C. Alteration of neutrophil reactive oxygen species production by extracts of devil’s claw (Harpagophytum). Manuscript submitted.
Place of Publication:Alnarp
Publisher:Department of Plant Breeding, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-8636-7
ISBN for electronic version:978-91-576-8637-4
ISSN:1652-6880
Language:English
Additional Information:NORDIC/SAREC Scholarship
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F30 Plant genetics and breeding
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biological Systematics
Agrovoc terms:pedaliaceae, drug plants, glycosides, antioxidants, antiinflammatory agents, genetic markers, chromosomes, cell division, hplc, rapd, botswana
Keywords:Harpagophytum, antioxidant, anti-inflammatory, devil’s claw, DNA marker, Harpagoside, HPLC, ISSR, Medicinal plant, RAPD, ROS
URN:NBN:urn:nbn:se:slu:epsilon-e-3435
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3435
ID Code:13374
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: Mr Mbaki Muzila
Deposited On:16 May 2016 12:58
Metadata Last Modified:17 May 2016 05:29

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