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High-Throughput Sequencing Shows High Fungal Diversity and Community Segregation in the Rhizospheres of Container-Grown Conifer Seedlings

Menkis, Audrius and Burokiene, Daiva and Stenlid, Jan and Stenström, Elna (2016). High-Throughput Sequencing Shows High Fungal Diversity and Community Segregation in the Rhizospheres of Container-Grown Conifer Seedlings. Forests. 7:2, 1-15
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Abstract

Forest nurseries in Sweden produce ca. 360 million seedlings of Pinus sylvestris L. and Picea abies (L.) Karst. annually. Fungi represent the largest microbial component in rhizospheres and may significantly affect health and, consequently, quality of the seedlings. The aim of this study was to assess fungi focusing on pathogens in roots and the sphagnum peat growth substrate of healthy-looking P. sylvestris and P. abies seedlings from nine forest nurseries situated in northern, central and southern regions of Sweden. We hypothesized that nursery stock and the growth substrate can provide a venue for dissemination of fungal diseases. In each nursery and for each tree species, 100 seedlings with the growth substrate were collected during the dormant period. DNA was isolated from parts of root systems and from samples of the growth substrate, amplified using internal transcribed spacer of rDNA as a marker and 454-sequenced. Clustering at 98.5% similarity of 169,844 high-quality sequences resulted in 619 non-singleton fungal taxa. Although results showed that management practices in forest nurseries generally give a healthy stock, latent establishment of pathogenic fungi in both roots and the growth substrate supported the hypothesis. Furthermore, seedling roots and the growth substrate were inhabited by distinct communities of fungi, and lifestyles of these fungi largely determined community segregation into particular ecological niche.

Authors/Creators:Menkis, Audrius and Burokiene, Daiva and Stenlid, Jan and Stenström, Elna
Title:High-Throughput Sequencing Shows High Fungal Diversity and Community Segregation in the Rhizospheres of Container-Grown Conifer Seedlings
Series/Journal:Forests (1999-4907)
Year of publishing :2016
Volume:7
Number:2
Page range:1-15
Number of Pages:15
Publisher:MDPI
ISSN:1999-4907
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Forest Science
Agrovoc terms:forestry
Keywords:forest nursery, mycorrhiza, pathogens, sequencing, seedlings, tree health
URN:NBN:urn:nbn:se:slu:epsilon-e-3545
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3545
Additional ID:
Type of IDID
Web of Science (WoS)000371896900005
DOI10.3390/f7020044
ID Code:13435
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology
Deposited By: SLUpub Connector
Deposited On:04 Jul 2016 07:00
Metadata Last Modified:04 Jul 2016 07:00

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