El-Namaky, Raafat and Sedeek, Saber and Dea Moukoumbi, Yonnelle and Ortiz Rios, Rodomiro Octavio and Manneh, Baboucarr
(2016).
Microsatellite-aided screening for fertility restorer genes (Rf) facilitates hybrid improvement.
Rice Science. 23:3, 160-164
[Journal article]
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Official URL: http://dx.doi.org/10.1016/j.rsci.2016.04.003
Abstract
DNA markers enabled to determine the chromosomal locations of the two Rf genes (Rf3 and Rf4) in the wild abortive-cytoplasmic male sterility (WA-CMS) system. Four simple sequence repeats (SSR or microsatellites) RM171, RM258, RM315 and RM443 were used to detect the allelic status with respect to the fertility restoring genes (Rf3 and Rf4) in 300 rice cultivars and breeding lines. Furthermore, 45 lines selected using SSR markers were mated with a CMS line (IR58025A) to analyze their restoring ability. The results revealed that 90 lines had Rf3, 65 lines had Rf4 and 45 lines had Rf3 and Rf4 alleles out of 300 lines screened. Offspring of all test lines except HHZ 8-SAL9DT1-Y1, HHZ 5-SAL9-Y3-1 and IDSA 77 exhibited high pollen and spikelet fertility (> 80%), thus confirming they bear Rf alleles. the hybrid offspring of ARH12-6-1-1-B-3-1, IR32307-10-3-2-1 and Sahel329 had the highest pollen and spikelet fertility, (95.1–97, 4%, and 97.1–98.3%, 97.1-96.1% respectively).
| Authors/Creators: | El-Namaky, Raafat and Sedeek, Saber and Dea Moukoumbi, Yonnelle and Ortiz Rios, Rodomiro Octavio and Manneh, Baboucarr |
|---|---|
| Title: | Microsatellite-aided screening for fertility restorer genes (Rf) facilitates hybrid improvement |
| Series/Journal: | Rice Science (1672-6308) |
| Year of publishing : | 2016 |
| Volume: | 23 |
| Number: | 3 |
| Page range: | 160-164 |
| Number of Pages: | 5 |
| Publisher: | China National Rice Research Institute, Elsevier |
| ISSN: | 1672-6308 |
| Language: | English |
| Publication Type: | Journal article |
| Refereed: | Yes |
| Article category: | Scientific peer reviewed |
| Version: | Published version |
| Full Text Status: | Public |
| Agris subject categories.: | F Plant production > F30 Plant genetics and breeding |
| Subjects: | (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology |
| Agrovoc terms: | rice, microsatellites, screening, plant breeding |
| Keywords: | cytoplasmic male sterility, fertility restoration gene, heterosis, rice, simple sequence repeat |
| URN:NBN: | urn:nbn:se:slu:epsilon-e-3595 |
| Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3595 |
| ID Code: | 13556 |
| Faculty: | LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap |
| Department: | (LTJ, LTV) > Department of Plant Breeding (from 130101) |
| Deposited By: | SLUpub Connector |
| Deposited On: | 18 Aug 2016 06:27 |
| Metadata Last Modified: | 18 Aug 2016 06:27 |
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