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Microsatellite-aided screening for fertility restorer genes (Rf) facilitates hybrid improvement

El-Namaky, Raafat and Sedeek, Saber and Dea Moukoumbi, Yonnelle and Ortiz Rios, Rodomiro Octavio and Manneh, Baboucarr (2016). Microsatellite-aided screening for fertility restorer genes (Rf) facilitates hybrid improvement. Rice Science. 23:3, 160-164
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Official URL: http://dx.doi.org/10.1016/j.rsci.2016.04.003

Abstract

DNA markers enabled to determine the chromosomal locations of the two Rf genes (Rf3 and Rf4) in the wild abortive-cytoplasmic male sterility (WA-CMS) system. Four simple sequence repeats (SSR or microsatellites) RM171, RM258, RM315 and RM443 were used to detect the allelic status with respect to the fertility restoring genes (Rf3 and Rf4) in 300 rice cultivars and breeding lines. Furthermore, 45 lines selected using SSR markers were mated with a CMS line (IR58025A) to analyze their restoring ability. The results revealed that 90 lines had Rf3, 65 lines had Rf4 and 45 lines had Rf3 and Rf4 alleles out of 300 lines screened. Offspring of all test lines except HHZ 8-SAL9DT1-Y1, HHZ 5-SAL9-Y3-1 and IDSA 77 exhibited high pollen and spikelet fertility (> 80%), thus confirming they bear Rf alleles. the hybrid offspring of ARH12-6-1-1-B-3-1, IR32307-10-3-2-1 and Sahel329 had the highest pollen and spikelet fertility, (95.1–97, 4%, and 97.1–98.3%, 97.1-96.1% respectively).

Authors/Creators:El-Namaky, Raafat and Sedeek, Saber and Dea Moukoumbi, Yonnelle and Ortiz Rios, Rodomiro Octavio and Manneh, Baboucarr
Title:Microsatellite-aided screening for fertility restorer genes (Rf) facilitates hybrid improvement
Series/Journal:Rice Science (1672-6308)
Year of publishing :2016
Volume:23
Number:3
Page range:160-164
Number of Pages:5
Publisher:China National Rice Research Institute, Elsevier
ISSN:1672-6308
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:F Plant production > F30 Plant genetics and breeding
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
Agrovoc terms:rice, microsatellites, screening, plant breeding
Keywords:cytoplasmic male sterility, fertility restoration gene, heterosis, rice, simple sequence repeat
URN:NBN:urn:nbn:se:slu:epsilon-e-3595
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3595
ID Code:13556
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:18 Aug 2016 06:27
Metadata Last Modified:18 Aug 2016 06:27

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