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Genetic characterisation of novel resistance alleles to stem rust and stripe rust in wheat-alien introgression lines

Rahmatov, Mahbubjon (2016). Genetic characterisation of novel resistance alleles to stem rust and stripe rust in wheat-alien introgression lines. Diss. (sammanfattning/summary) Alnarp : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2016:78
ISBN 978-91-576-8658-9
eISBN 978-91-576-8659-6
[Doctoral thesis]

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Abstract

Bread wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD) is one of the most important food crops world-wide, but is attacked by many diseases and pests that cause significant yield losses. Globally, stem rust (Sr) (Puccinia graminis f. sp. tritici Erikss & E. Henning), stripe rust (Yr) (Puccinia striiformis Westend. f. sp. tritici Eriks) and leaf rust (Lr) (Puccinia triticina Eriks) are a great threat to wheat production. The majority of the Sr, Yr and Lr resistance genes are already defeated by numerous virulent races, so enhanced genetic resistance against these devastating diseases are essential. Wheat-alien introgressions from derivatives of Secale cereale L. (2n = 2x = 14, RR), Leymus mollis (2n = 4x = 28, NsNsXmXm), Leymus racemosus (2n = 4x = 28, NsXm) and Thinopyrum junceiforme (2n = 4x = 28; J1J1J2J2) are important genetic resources for new sources of resistance genes. To identify new sources of resistance, this thesis evaluated seedling and adult plant resistance to a wide array of stem rust and stripe rust races. Three wheat-rye disomic substitution lines 2R (2D) were found to carry new resistance gene/s to stem rust races and six multiple wheat-rye introgression lines with 5RS·5AL+4R+6R carried new resistance gene/s to stripe rust races. At adult plant stage, the wheat-rye translocation line with 1BL·1RS and 2RL·2BS exhibited low susceptibility to race TTKSK under field conditions.

The wheat-rye T2DS·2RL Robertsonian translocation line (TA5094) with a new stem rust resistance gene was developed through the breakage-fusion mechanism and verified using seedling resistance assays and molecular and cytogenetic analyses. Three kompetitive allele-specific PCR (KASP) markers located on rye chromosome 2RL were identified as being closely associated with the new stem rust resistance gene. Fluorescence in situ hybridisation (FISH) analysis confirmed the resistance gene in F3:4 homozygous lines. The stem rust resistance gene in TA5094 line on chromosome 2RL arm was designated Sr59.

Wheat cultivars, advanced lines and landraces from Tajikistan were assessed at seedling and adult plant stages against Sr, Yr and Lr races. Based on multipathotype assessment and molecular markers, the presence of Sr6, Sr31/Yr9/Lr26, Sr38/Yr17/Lr37, Yr2 and Yr27 and pleiotropic resistance genes Sr57/Lr34/Yr18/ and Sr2/Yr30/Lr27 was postulated.

Overall, this thesis identified novel genetic resistance resources against stem rust, stripe rust and leaf rust in Tajik wheat and in wheat-alien introgressions. This resistance gene/s will be useful in diversifying the current set of resistance genes deployed to control these devastating diseases.

Authors/Creators:Rahmatov, Mahbubjon
Title:Genetic characterisation of novel resistance alleles to stem rust and stripe rust in wheat-alien introgression lines
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :22 August 2016
Depositing date:22 August 2016
Volume:2016:78
Number of Pages:76
Papers/manuscripts:
NumberReferences
IRahmatov, M., M.N. Rouse, B.J. Steffenson, S.C. Andersson, R. Wanyera, Z.A. Pretorius, A. Houben, K. Nazari, S. Bhavani, and E. Johansson. 2016a. Sources of stem rust resistance in wheat-alien introgression lines. Plant Disease 100: 1101-1109. doi:10.1094/PDIS-12-15-1448-RE.
IIRahmatov, M., M.N. Rouse, J. Nirmala, T. Danilova, B. Friebe, B.J. Steffenson, and E. Johansson. 2016b. A new 2DS·2RL Robertsonian translocation transfers stem rust resistance gene Sr59 into wheat. Theoretical and Applied Genetics 129: 1383-1392. doi:10.1007/s00122-016-2710-6.
IIIRahmatov, M., L. Garkava-Gustavsson, R. Wanyera, B. Steffenson, M.N. Rouse and E. Johansson. 2015. Stem rust resistance in 1BL·1RS and 2RL·2BS double wheat-rye translocation lines. Czech Journal of Genetics and Plant Breeding 51: 148-154.
IVRahmatov, M., M. Hovmøller, K. Nazari, S.C. Andersson, M.N. Rouse, B.J. Steffenson and E. Johansson. Seedling and adult plant stripe rust resistances in diverse wheat-alien introgression lines. (Submitted)
VRahmatov, M., M. Otambekova, H. Muminjanov, M.N. Rouse, M. Hovmøller, K. Nazari, B.J. Steffenson, J.A. Kolmer and E. Johansson. Stem, stripe and leaf rust seedling and adult plant resistances in Tajik bread wheat. (Manuscript)
Place of Publication:Alnarp
Publisher:Department of Plant Breeding, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-8658-9
ISBN for electronic version:978-91-576-8659-6
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding
Agrovoc terms:chromosome manipulation, triticum aestivum, puccinia striiformis, puccinia graminis, puccinia triticina, translocation, secale cereale, disease resistance, introgression
Keywords:Chromosome engineering, Gene postulation, KASP,, ph1b,, Secale cereale, Triticum aestivum, translocation, Ug99, Leymus
URN:NBN:urn:nbn:se:slu:epsilon-e-3620
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3620
ID Code:13585
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
External funders:Monsanto's Beachell-Borlaug International Scholars Program
Deposited By: Mr Mahbubjon Rahmatov
Deposited On:23 Aug 2016 08:11
Metadata Last Modified:07 Sep 2016 13:31

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