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Canine C-reactive protein

validation of two automated canine-specific C-reactive protein assays and studies on clinical and research applications

Hillström, Anna (2016). Canine C-reactive protein. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2016:79
ISBN 978-91-576-8660-2
eISBN 978-91-576-8661-9
[Doctoral thesis]

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Abstract

C-reactive protein (CRP) is a sensitive and specific marker of systemic inflammation in dogs, valuable for diagnosing and monitoring inflammatory diseases. The use of CRP in canine medicine has however been hampered by the lack of automated assays optimized for measuring CRP in this species. The need for improved CRP assays was the reason for initiating the current project, with the goal to generate automated, canine-specific CRP tests that could reliably measure serum CRP over the whole concentration range expected to occur in dogs. Two different assays were developed for this purpose. One was designed for routine diagnostic testing, and one was a high-sensitivity CRP test intended for research. Method validation studies were performed, demonstrating that both tests met the predefined quality criteria. Using the two novel CRP tests, it was possible to reliably measure serum CRP concentrations in the range of 0.5-1200 mg/l. After successful termination of the validation studies, the CRP assays were used in clinical research studies. C-reactive protein concentrations were measured in dogs with pyometra undergoing ovariohysterectomy, to evaluate how surgical treatment affected degree of systemic inflammation in these patients. Two other studies were performed to evaluate the usefulness of CRP as a diagnostic test. C-reactive protein concentration was found to discriminate well between dogs with suppurative arthritis and dogs with osteoarthritis, whereas measurement of CRP was not efficient for diagnosing late post-operative bacterial infections after orthopaedic surgery because these infections often did not elicit a systemic inflammatory response.

In conclusion, two novel automated canine-specific CRP assays were developed and validated with satisfactory results. The tests showed high practicability for measuring CRP in samples from clinical research studies. Availability of these assays will facilitate the use of CRP as a routine diagnostic test in veterinary medicine, and can improve quality in research on canine inflammatory diseases.

Authors/Creators:Hillström, Anna
Title:Canine C-reactive protein
Subtitle:validation of two automated canine-specific C-reactive protein assays and studies on clinical and research applications
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :August 2016
Depositing date:August 2016
Volume:2016:79
Number of Pages:78
Papers/manuscripts:
NumberReferences
IHillström A, Hagman R, Tvedten H, Kjelgaard-Hansen M (2014). Validation of a commercially available automated canine-specific immunoturbidimetric method for measuring canine C-reactive protein. Vet Clin Pathol 43(2), 235-243.
IIHillström A, Hagman R, Tvedten H, Kjelgaard-Hansen M (2014). Validation of a commercially available automated canine-specific immunoturbidimetric method for measuring canine C-reactive protein. Vet Clin Pathol 43(2), 235-243.
IIIBrandeker E, Hillström A, Hanås S, Hagman R, Ström Holst B (2015). The effect of a single dose of prednisolone in dogs envenomated by Vipera berus – a randomized, double-blind, placebo-controlled clinical trial. BMC Vet Res 11(44).
IVHillström A, Bylin J, Hagman R, Björhall K, Tvedten H, Königsson K, Fall T, Kjelgaard-Hansen M (2016). Measurement of serum C-reactive protein for discriminating between suppurative arthritis and osteoarthritis in dogs (submitted manuscript).
Place of Publication:Uppsala
Publisher:Department of Clinical Sciences, Swedish Universityof Agricultural Sciences
ISBN for printed version:978-91-576-8660-2
ISBN for electronic version:978-91-576-8661-9
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:L Animal production > L73 Animal diseases
Subjects:(A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 302 Clinical Medicine > Clinical Laboratory Medicine
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Agrovoc terms:dogs, systemic diseases, inflammation, genetic markers, osteoarthritis, snake bites, immunological techniques, pyometra
Keywords:C-reactive protein, Method validation, Acute phase protein, Inflammation, Immunoturbidimetric assay
URN:NBN:urn:nbn:se:slu:epsilon-e-3628
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3628
ID Code:13597
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
External funders:Swedish Agency for Innovation Systems
Deposited By: DVM Anna Hillstrom
Deposited On:25 Aug 2016 14:22
Metadata Last Modified:08 Sep 2016 05:48

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