Jonsson, Anders and Marzec-Schmidt, Katarzyna and Börjesson, Gunnar and Wallenhammar, Ann-Charlotte and Wallenhammar, Ann-Charlotte
(2016).
Quantitative PCR shows propagation of Plasmodiophora brassicae in Swedish long term field trials.
European journal of plant pathology. 145
:3
, 573-581
[Research article]
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Abstract
Clubroot (Plasmodiophora brassicae) is a serious soil-borne disease in brassica crops world-wide. We report on a time series of soil samples from Swedish long-term fertility trials started in 1957, 1963 and 1966, which were analyzed for the amount ofP. brassicaeDNA. The crop rotations included a brassica crop every 4 or 6years. All experimental sites with a 4-year rotation of oilseed rape, except one with calcium carbonate in the soil profile, showed high (>1000fg DNA g−1soil) levels ofP. brassicaeDNA after 9, 11 and 12 rotations. In contrast, detectable levels (>5fg DNA g−1soil) ofP. brassicaewere found only at one of five sites with a 6-year rotation of spring oilseed rape. In years with high levels ofP. brassicaeDNA, low yield was reported and a subsequent decline inP. brassicaeDNA in soil was observed. Different NPK (nitrogen/phosphorus/potassium) fertiliser regimes resulted in similarP. brassicaeDNA levels. The robustness and reliability of the method applied was verified by analyses of soil from individual plots compared with a mixture of plots and by repeated analyses of selected samples, which showed thatP. brassicaeDNA remained stable during dry storage.
| Authors/Creators: | Jonsson, Anders and Marzec-Schmidt, Katarzyna and Börjesson, Gunnar and Wallenhammar, Ann-Charlotte and Wallenhammar, Ann-Charlotte | ||||||
|---|---|---|---|---|---|---|---|
| Title: | Quantitative PCR shows propagation of Plasmodiophora brassicae in Swedish long term field trials | ||||||
| Series Name/Journal: | European journal of plant pathology | ||||||
| Year of publishing : | 2016 | ||||||
| Volume: | 145 | ||||||
| Number: | 3 | ||||||
| Page range: | 573-581 | ||||||
| Number of Pages: | 9 | ||||||
| Publisher: | Springer | ||||||
| ISSN: | 0929-1873 | ||||||
| Language: | English | ||||||
| Publication Type: | Research article | ||||||
| Refereed: | Yes | ||||||
| Article category: | Scientific peer reviewed | ||||||
| Version: | Published version | ||||||
| Copyright: | Creative Commons: Attribution 4.0 | ||||||
| Full Text Status: | Public | ||||||
| Subjects: | (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Soil Science | ||||||
| Agrovoc terms: | PCR, soil analysis, crop rotation | ||||||
| Keywords: | long-term fertility, field trials, Clubroot, Soil samples, Plasmodiophora brassicae, Quantitative PCR, qPCR | ||||||
| URN:NBN: | urn:nbn:se:slu:epsilon-e-3633 | ||||||
| Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3633 | ||||||
| Additional ID: |
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| ID Code: | 13607 | ||||||
| Faculty: | NJ - Fakulteten för naturresurser och jordbruksvetenskap S - Faculty of Forest Sciences | ||||||
| Department: | (NL, NJ) > Dept. of Soil and Environment (S) > Dept. of Soil and Environment | ||||||
| Deposited By: | SLUpub Connector | ||||||
| Deposited On: | 31 Aug 2016 07:04 | ||||||
| Metadata Last Modified: | 09 Sep 2020 14:17 |
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