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Quantitative PCR shows propagation of Plasmodiophora brassicae in Swedish long term field trials

Jonsson, Anders and Marzec-Schmidt, Katarzyna and Börjesson, Gunnar and Wallenhammar, Ann-Charlotte and Wallenhammar, Ann-Charlotte (2016). Quantitative PCR shows propagation of Plasmodiophora brassicae in Swedish long term field trials. European journal of plant pathology. 145:3, 573-581
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Abstract

Clubroot (Plasmodiophora brassicae) is a serious soil-borne disease in brassica crops world-wide. We report on a time series of soil samples from Swedish long-term fertility trials started in 1957, 1963 and 1966, which were analyzed for the amount ofP. brassicaeDNA. The crop rotations included a brassica crop every 4 or 6years. All experimental sites with a 4-year rotation of oilseed rape, except one with calcium carbonate in the soil profile, showed high (>1000fg DNA g−1soil) levels ofP. brassicaeDNA after 9, 11 and 12 rotations. In contrast, detectable levels (>5fg DNA g−1soil) ofP. brassicaewere found only at one of five sites with a 6-year rotation of spring oilseed rape. In years with high levels ofP. brassicaeDNA, low yield was reported and a subsequent decline inP. brassicaeDNA in soil was observed. Different NPK (nitrogen/phosphorus/potassium) fertiliser regimes resulted in similarP. brassicaeDNA levels. The robustness and reliability of the method applied was verified by analyses of soil from individual plots compared with a mixture of plots and by repeated analyses of selected samples, which showed thatP. brassicaeDNA remained stable during dry storage.

Authors/Creators:Jonsson, Anders and Marzec-Schmidt, Katarzyna and Börjesson, Gunnar and Wallenhammar, Ann-Charlotte and Wallenhammar, Ann-Charlotte
Title:Quantitative PCR shows propagation of Plasmodiophora brassicae in Swedish long term field trials
Series/Journal:European journal of plant pathology (0929-1873)
Year of publishing :2016
Volume:145
Number:3
Page range:573-581
Number of Pages:9
Publisher:Springer
ISSN:0929-1873
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Soil Science
Agrovoc terms:PCR, soil analysis, crop rotation
Keywords:long-term fertility, field trials, Clubroot, Soil samples, Plasmodiophora brassicae, Quantitative PCR, qPCR
URN:NBN:urn:nbn:se:slu:epsilon-e-3633
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3633
Additional ID:
Type of IDID
Web of Science (WoS)000377403200006
DOI10.1007/s10658-016-0906-x
ID Code:13607
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
S - Faculty of Forest Sciences
Department:(NL, NJ) > Dept. of Soil and Environment
(S) > Dept. of Soil and Environment
Deposited By: SLUpub Connector
Deposited On:31 Aug 2016 07:04
Metadata Last Modified:31 Aug 2016 07:04

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