Kalynych, Sergei and Přidal, Antonín and Pálková, Lenka and Levdansky, Yevgen and Rodrigues De Miranda, Joachim and Plevka, Pavel
(2016).
Virion structure of Iflavirus Slow bee paralysis virus at 2.6-Angstrom resolution.
Journal of virology. 90
:16
, 7444 –7455
[Research article]
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PDF
- Accepted Version
Available under License Creative Commons Attribution. 41MB |
Official URL: http://dx.doi.org/10.1128/JVI.00680-16
Abstract
The western honeybee (Apis mellifera) is the most important commercial insect pollinator. However, bees are under pressure from habitat loss, environmental stress, and pathogens, including viruses that can cause lethal epidemics. Slow bee paralysis virus (SBPV) belongs to the Iflaviridae family of nonenveloped single-stranded RNA viruses. Here we present the structure of the SBPV virion determined from two crystal forms to resolutions of 3.4 angstrom and 2.6 angstrom. The overall structure of the virion resembles that of picornaviruses, with the three major capsid proteins VP1 to 3 organized into a pseudo-T3 icosahedral capsid. However, the SBPV capsid protein VP3 contains a C-terminal globular domain that has not been observed in other viruses from the order Picornavirales. The protruding (P) domains form "crowns" on the virion surface around each 5-fold axis in one of the crystal forms. However, the P domains are shifted 36 angstrom toward the 3-fold axis in the other crystal form. Furthermore, the P domain contains the Ser-His-Asp triad within a surface patch of eight conserved residues that constitutes a putative catalytic or receptor-binding site. The movements of the domain might be required for efficient substrate cleavage or receptor binding during virus cell entry. In addition, capsid protein VP2 contains an RGD sequence that is exposed on the virion surface, indicating that integrins might be cellular receptors of SBPV.IMPORTANCEPollination by honeybees is needed to sustain agricultural productivity as well as the biodiversity of wild flora. However, honey-bee populations in Europe and North America have been declining since the 1950s. Honeybee viruses from the Iflaviridae family are among the major causes of honeybee colony mortality. We determined the virion structure of an Iflavirus, slow bee paralysis virus (SBPV). SBPV exhibits unique structural features not observed in other picorna-like viruses. The SBPV capsid protein VP3 has a large C-terminal domain, five of which form highly prominent protruding "crowns" on the virion surface. However, the domains can change their positions depending on the conditions of the environment. The domain includes a putative catalytic or receptor binding site that might be important for SBPV cell entry.
Authors/Creators: | Kalynych, Sergei and Přidal, Antonín and Pálková, Lenka and Levdansky, Yevgen and Rodrigues De Miranda, Joachim and Plevka, Pavel | ||||
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Title: | Virion structure of Iflavirus Slow bee paralysis virus at 2.6-Angstrom resolution | ||||
Series Name/Journal: | Journal of virology | ||||
Year of publishing : | 2016 | ||||
Volume: | 90 | ||||
Number: | 16 | ||||
Page range: | 7444 –7455 | ||||
Number of Pages: | 12 | ||||
Publisher: | American Society for Microbiology | ||||
Associated Programs and Other Stakeholders: | SLU - Research Areas for the Future > Future Animal Health and Welfare (until Jan 2017) | ||||
ISSN: | 1098-5514 | ||||
Language: | English | ||||
Publication Type: | Research article | ||||
Refereed: | Yes | ||||
Article category: | Scientific peer reviewed | ||||
Version: | Accepted version | ||||
Copyright: | Creative Commons: Attribution 4.0 | ||||
Full Text Status: | Public | ||||
Agris subject categories.: | L Animal production > L20 Animal ecology L Animal production > L73 Animal diseases | ||||
Subjects: | (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Structural Biology (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Cell Biology | ||||
Agrovoc terms: | viruses, Apis mellifera | ||||
Keywords: | colony collapse disorder, CCD, virus, Apis mellifera, honey bee, honeybee, bumblebee, Picornavirales, Iflaviridae, Iflavirus, picornavirus, virion, structure, X-ray, crystal, capsid, protein, jellyroll, inhibitor, antiviral, domain, catalytic site | ||||
URN:NBN: | urn:nbn:se:slu:epsilon-e-3744 | ||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3744 | ||||
Additional ID: |
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ID Code: | 13728 | ||||
Faculty: | NJ - Fakulteten för naturresurser och jordbruksvetenskap | ||||
Department: | (NL, NJ) > Dept. of Ecology (S) > Dept. of Ecology | ||||
Deposited By: | SLUpub Connector | ||||
Deposited On: | 28 Oct 2016 08:26 | ||||
Metadata Last Modified: | 03 Jan 2022 09:41 |
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