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Virion Structure of Israeli Acute Bee Paralysis Virus

Mullapudi, Edukondalu and Přidal, Antonín and Pálková, Lenka and Rodrigues De Miranda, Joachim and Plevka, Pavel (2016). Virion Structure of Israeli Acute Bee Paralysis Virus. Journal of virology. 90:18, 8150 –8159
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Abstract

The pollination services provided by the western honeybee (Apis mellifera) are critical for agricultural production and the diversity of wild flowering plants. However, honeybees suffer from environmental pollution, habitat loss, and pathogens, including viruses that can cause fatal diseases. Israeli acute bee paralysis virus (IAPV), from the family Dicistroviridae, has been shown to cause colony collapse disorder in the United States. Here, we present the IAPV virion structure determined to a resolution of 4.0 angstrom and and the structure of a pentamer of capsid protein protomers at a resolution of 2.7 angstrom. IAPV has major capsid proteins VP1 and VP3 with noncanonical jellyroll beta-barrel folds composed of only seven instead of eight beta-strands, as is the rule for proteins of other viruses with the same fold. The maturation of dicistroviruses is connected to the cleavage of precursor capsid protein VP0 into subunits VP3 and VP4. We show that a putative catalytic site formed by the residues Asp-Asp-Phe of VP1 is optimally positioned to perform the cleavage. Furthermore, unlike many picornaviruses, IAPV does not contain a hydrophobic pocket in capsid protein VP1 that could be targeted by capsid-binding antiviral compounds.
Honeybee pollination is required for agricultural production and to sustain the biodiversity of wild flora. However, honeybee populations in Europe and North America are under pressure from pathogens, including viruses that cause colony losses. Viruses from the family Dicistroviridae can cause honeybee infections that are lethal, not only to individual honeybees, but to whole colonies. Here, we present the virion structure of an Aparavirus, Israeli acute bee paralysis virus (IAPV), a member of a complex of closely related viruses that are distributed worldwide. IAPV exhibits unique structural features not observed in other picorna-like viruses. Capsid protein VP1 of IAPV does not contain a hydrophobic pocket, implying that capsid-binding antiviral compounds that can prevent the replication of vertebrate picornaviruses may be ineffective against honeybee virus infections.

Authors/Creators:Mullapudi, Edukondalu and Přidal, Antonín and Pálková, Lenka and Rodrigues De Miranda, Joachim and Plevka, Pavel
Title:Virion Structure of Israeli Acute Bee Paralysis Virus
Series/Journal:Journal of virology (1098-5514)
Year of publishing :2016
Volume:90
Number:18
Page range:8150 –8159
Number of Pages:10
Publisher:American Society for Microbiology
Associated Programs and Other Stakeholders:SLU - Research Areas for the Future > Future Animal Health and Welfare
ISSN:1098-5514
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Structural Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Cell Biology
Agrovoc terms:Apis mellifera, Viroses
Keywords:Honey bees, Apis Mellifera, Israeli acute bee paralysis virus (IAPV), Colony collapse disorder, Poliovirus, Picornaviruses, Dicistroviridae
URN:NBN:urn:nbn:se:slu:epsilon-e-3768
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3768
Additional ID:
Type of IDID
Web of Science (WoS)000382314100011
DOI10.1128/JVI.00854-16
ID Code:13735
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Ecology
(S) > Dept. of Ecology
Deposited By: SLUpub Connector
Deposited On:08 Nov 2016 10:36
Metadata Last Modified:08 Nov 2016 12:07

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