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Sucrose cleavage pathways in aspen wood

Rende, Umut (2016). Sucrose cleavage pathways in aspen wood. Diss. (sammanfattning/summary) Umeå : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2016:110
ISBN 978-91-576-8723-4
eISBN 978-91-576-8724-1
[Doctoral thesis]

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Abstract

Cellulose is the main component of wood and one of the most important renewable raw materials. In several tree species including Populus species, carbon for cellulose biosynthesis is derived from the disaccharide sucrose. This thesis describes experimental work on the mechanism of sucrose cleavage in developing wood and subsequent production of UDP-glucose (UDP-Glc) for cellulose biosynthesis.

Sucrose synthase (SUS) has been proposed previously to interact directly with cellulose synthase complexes (CSC) and specifically supply UDP-Glc for cellulose biosynthesis. To investigate the role of SUS in wood biosynthesis, transgenic lines of hybrid aspen (Populus tremula L. x tremuloides Michx.) with strongly reduced soluble SUS activity in developing wood were characterized. The reduction of soluble SUS activity to few percentage of wild type increased soluble sugar content but decreased wood density and consequently reduced the lignin, hemicellulose and cellulose content per volume of wood. The results demonstrate that SUS has an important role in carbon flux from sucrose to all wood polymers but has no specific role in supplying UDP-Glc to cellulose synthesis machinery.

I also investigated the role of cytosolic neutral/alkaline invertases (cNINs) during cellulose biosynthesis in hybrid aspen by analysing transgenic lines where NIN activity was decreased during secondary cell wall formation. The decrease in NINs activity caused a reduction in UDP-Glc and consequently reduced crystalline cellulose content but increased amorphous cellulose in cellulose microfibrils of wood. The results in this study demonstrated that cNIN activity is a major rate-controlling step in the cellulose biosynthesis.

There is a lack of global analytical methods to measure sugar phosphates linked to cell wall polymer biosynthesis. To address this problem, I worked with the UPSC metabolomics facility to develop a robust method based on chloroform/methanol extraction, two-step derivatization and detection using reverse phase liquid chromatography-mass spectrometry (RP-LC-MS) without adding ion-pairing reagent. The method could quantitatively identify 18 sugar phosphates including UDP-Glc and structural isomers in Populus leaf and wood extracts. The method can now be used to gain deeper understanding into wood metabolism and cell wall biosynthesis.

Authors/Creators:Rende, Umut
Title:Sucrose cleavage pathways in aspen wood
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :2016
Depositing date:11 November 2016
Volume:2016:110
Number of Pages:60
Papers/manuscripts:
NumberReferences
ILorenz Gerber, Bo Zhang, Melissa Roach, Umut Rende, Andras Gorzsas, Manoj Kumar, Ingo Burgert, Totte Niittylä and Bjorn Sundberg (2014). Deficient sucrose synthase activity in developing wood does not specifically affect cellulose biosynthesis, but causes an overall decrease in cell wall polymers. New Phytologist, 203:1220-1230.
IIUmut Rende, Wei Wang, Madhavi Latha Gandla, Leif J. Jönsson and Totte Niittylä (2016). Cellulose biosynthesis in wood relies on cytosolic invertase activity. New Phytologist, in press.
IIIUmut Rende, Totte Niittylä and Thomas Moritz (2016). Determination of sugar phosphates in plants using combined reversed phase chromatography and tandem mass spectrometry. Manuscript.
Place of Publication:Umeå
Publisher:Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-8723-4
ISBN for electronic version:978-91-576-8724-1
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F60 Plant physiology and biochemistry
K Forestry > K01 Forestry - General aspects
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Forest Science
Agrovoc terms:sucrose, cellulose, biosynthesis, populus tremula, populus tremuloides, hybrids, wood, sugar phosphates, glycosidases, chromatography, mass spectrometry
Keywords:Cellulose, sucrose synthase, invertase, cellulose synthase complex, UDP-glucose, UHPLC-MS/MS
URN:NBN:urn:nbn:se:slu:epsilon-e-3787
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3787
ID Code:13844
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: PhD Umut Rende
Deposited On:16 Nov 2016 10:54
Metadata Last Modified:17 Nov 2016 06:52

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