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Anti-cancer effects of bioactive compounds from rose hip fruit in human breast cancer cell lines

carotenoids, triterpenes, and ascorbate evaluated singly or in combination

Zhong, Lijie (2017). Anti-cancer effects of bioactive compounds from rose hip fruit in human breast cancer cell lines. Diss. (sammanfattning/summary) Alnarp : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2017:8
ISBN 978-91-576-8789-0
eISBN 978-91-576-8790-6
[Doctoral thesis]

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Abstract

Rose hips have long been used in human diets as a food ingredient and supplement. Their multiple medical properties, which have been attributed to their abundant carotenoid composition, have attracted widespread scientific attention. This thesis examined the carotenoid composition in rose hips from five rose species. The anti-cancer effect of different carotenoid fractions from rose hips was investigated in human breast cancer cell lines, using the natural variation in carotenoid content in hips from different rose species. Based on the results obtained, representative single carotenoids were selected for further investigation.

A rapid, effective method was developed using a HPLC-DAD-APCI+-MS system for carotenoid identification and quantification in rose hips. Twenty-one carotenoids, including 11 xanthophylls and 10 carotenes, were detected in saponified extract and 23 carotenoid esters in unsaponified extract from hips of the five rose species. Three fractions were subsequently isolated from total carotenoid extract of rose hips and their anti-proliferative activities were investigated in human breast cancer cell lines. Xanthophyll ester fraction was proved more potent than lycopene isomer or an undefined carotene fraction. Rosa multiflora Thunb. hips were the most effective, giving low IC50 values in MTT assay, and a unique xanthophyll ester pattern was found in this species.

When xanthophyll ester was combined with triterpenes in MTT assay, synergistic effects were found in the MCF-7 cancer cell line. A significant synergistic effect was also found on combining a 10 µM concentration of rose hip triterpene fraction with 50 µM ascorbate, which resulted in strong inhibition in cell proliferation in MCF-7 cell line, while normal-like cells MCF-10A were relatively undamaged.

Xanthophyll esters were verified to be more effective than free xanthophylls in MTT assay comparing lutein and zeaxanthin with their esters. The xanthophyll ester fraction from R. multiflora and individual xanthophyll esters were found to decrease cancer stem cell sub-populations and inhibit cell migration. Xanthophyll esters were suggested to affect breast cancer cells by a mechanism involving the NF-κB pathway.

Overall, these results support the beneficial health effects of rose hips and reveal potential to involve xanthophyll esters in future breast cancer therapy.

Authors/Creators:Zhong, Lijie
Title:Anti-cancer effects of bioactive compounds from rose hip fruit in human breast cancer cell lines
Subtitle:carotenoids, triterpenes, and ascorbate evaluated singly or in combination
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :10 February 2017
Depositing date:3 February 2017
Volume:2017:8
Number of Pages:61
Papers/manuscripts:
NumberReferences
I.Zhong, L., Gustavsson, K.E., Oredsson, S., Głąb, B., Yilmaz, J.L. & Olsson, M.E. (2016). Determination of free and esterified carotenoid composition in rose hip fruit by HPLC-DAD-APCI+-MS. Food Chemistry 210, 541-550.
II.Zhong, L., Olsson, M.E., Huang, X., Gustavsson, K.E. & Oredsson, S. Cancer phenotype-specific effect of the xanthophyll ester fraction extracted from rose hips in a human breast cancer cell line. (Submitted)
III.Zhong, L., Paulsen, F., Strand, D., Oredsson, S. & Olsson, M.E. Anti-cancer activities of lutein dimyristate and zeaxanthin dimyristate in human breast cancer cell lines and a breast epithelial cell line. (Manuscript)
IV.Zhong, L., Oredsson, S. & Olsson, M.E. Effects of combining triterpenes, lutein dimyristate and ascorbate on cell proliferation in human breast cancer cell lines and a breast epithelial cell line. (Manuscript)
Place of Publication:Alnarp
Publisher:Department of Plant Breeding, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-8789-0
ISBN for electronic version:978-91-576-8790-6
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:Q Food science > Q01 Food science and technology
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 104 Chemical Sciences > Analytical Chemistry
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Cell Biology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Horticulture
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Food Science
Agrovoc terms:rose hips, fruits, xanthophyllis, carotenoids, triterpenoids, human diseases, neoplasms, therapy, drug plants
Keywords:rose hip, fruit, xanthophyll ester, Rosa sp., breast cancer, JIMT-1, MCF-7, MCF-10A, MTT assay, anti-proliferative, synergistic effect, cancer stem cell
URN:NBN:urn:nbn:se:slu:epsilon-e-3926
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3926
ID Code:13921
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
External funders:China Scholarship Council
Deposited By: Lijie Zhong
Deposited On:06 Feb 2017 09:46
Metadata Last Modified:06 Feb 2017 09:46

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