Chanrot, Metasu
(2017).
Responses of bovine endometrial epithelial cells to pathogens.
Diss. (sammanfattning/summary)
Uppsala :
Sveriges lantbruksuniv.,
Acta Universitatis Agriculturae Sueciae, 1652-6880
; 2017:16
ISBN 978-91-576-8805-7
eISBN 978-91-576-8806-4
[Doctoral thesis]
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Abstract
In dairy cows, clinical uterine infection (metritis) and subsequent persistent inflammation of the endometrium (endometritis) are major causes of infertility. Escherichia coli (E. coli) is a prevalent bacteria in metritis and endometritis and promotes infection with bovine herpes virus type 4 (BoHV-4) through mechanisms involving lipopolysaccharide endotoxins (LPS). In this thesis, interactions between E. coli LPS, BoHV-4 and the endometrial epithelium were studied using in vitro models following characterisation of tissue samples used for culture. Examination of cell proliferation, survival and apoptosis after challenges with various doses of LPS revealed that cow and tissue characteristics did not influence proliferation of bovine endometrial epithelial cells (bEEC) in response to LPS. However, E. coli LPS stimulated proliferation of bEEC (maximum observed at 8 µg/mL LPS). The strong increase in cell numbers by 72 h was not associated with an increase in apoptosis, but this occurred with higher LPS doses. Analysis of protein pro-files revealed de-regulation of 38 proteins belonging to many pathways, some related to the process of implantation. Morphological studies and ELISA were used to characterise the survival of cells and the cytokine response of bEEC to BoHV-4. In infected samples, the number of living cells started to decrease by Day 4 post-challenge and by Day 7 the number was lower than in controls. This change was associated with viral replication between Day 0 and Day 5, as demonstrated by immunofluorescence, titration and quantitative polymerase chain reaction (qPCR) results and changes in IL-8 and TNF-α profiles. Moreover, the results showing strong pathogenic effects of BoHV-4 on endometrial epithelial cells pave the way for future studies on sexual transmission of BoHV-4 at time of insemination.
The results obtained led to development of reliable models to study interactions between uterine epithelial cells and pathogens, which could be of translational use. In a time- and dose-dependent manner, E. coli LPS increased and BoHV-4 decreased the survival of bovine bEEC in vitro, while LPS induced strong alterations of protein profiles, especially those related to pathways activated at time of implantation. Such de-regulations may be part of the mechanism by which persistent inflammation following infection impairs fertility. This information can be exploited to identify new diagnostic markers of persistence of inflammation in the endometrium.
Authors/Creators: | Chanrot, Metasu | ||||||||
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Title: | Responses of bovine endometrial epithelial cells to pathogens | ||||||||
Series Name/Journal: | Acta Universitatis Agriculturae Sueciae | ||||||||
Year of publishing : | 2017 | ||||||||
Depositing date: | 2017 | ||||||||
Number: | 2017:16 | ||||||||
Number of Pages: | 80 | ||||||||
Papers/manuscripts: |
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Place of Publication: | Uppsala | ||||||||
Publisher: | Department of Clinical Sciences, Swedish University of Agricultural Sciences | ||||||||
ISBN for printed version: | 978-91-576-8805-7 | ||||||||
ISBN for electronic version: | 978-91-576-8806-4 | ||||||||
ISSN: | 1652-6880 | ||||||||
Language: | English | ||||||||
Publication Type: | Doctoral thesis | ||||||||
Full Text Status: | Public | ||||||||
Agris subject categories.: | L Animal production > L53 Animal physiology - Reproduction L Animal production > L73 Animal diseases | ||||||||
Subjects: | (A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 302 Clinical Medicine > Obstetrics, Gynaecology and Reproductive Medicine (A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 304 Medical Biotechnology > Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science | ||||||||
Agrovoc terms: | dairy cows, endometrium, endometritis, epithelium, cell culture, oestrous cycle, inflammation, pathogens, in vitro experimentation, laboratory diagnosis | ||||||||
Keywords: | cow, LPS, BoHV-4, endometrium, cell culture, proteomics, cell proliferation, endometritis, oestrous cycle | ||||||||
URN:NBN: | urn:nbn:se:slu:epsilon-e-3998 | ||||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-3998 | ||||||||
ID Code: | 14124 | ||||||||
Faculty: | VH - Faculty of Veterinary Medicine and Animal Science | ||||||||
Department: | (VH) > Dept. of Clinical Sciences | ||||||||
External funders: | Rajamangala University of Technology Srivijaya | ||||||||
Deposited By: | Miss Metasu Chanrot | ||||||||
Deposited On: | 02 Mar 2017 12:27 | ||||||||
Metadata Last Modified: | 10 Sep 2020 13:41 |
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