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Metabolomics as a tool to evaluate salmonid response to alternative feed ingredients

Cheng, Ken (2017). Metabolomics as a tool to evaluate salmonid response to alternative feed ingredients. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2017:33
ISBN 978-91-576-8839-2
eISBN 978-91-576-8840-8
[Doctoral thesis]

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Abstract

Aquaculture has largely expanded in the last decades to satisfy the growing market demands for fish products. Fishmeal and fish oil, which are traditionally used in salmonid feeds, are becoming unsustainable. Development of aquafeeds based on alternative ingredients are needed to overcome the ecological challenges. Importantly, when using the new diets, fish growth performance, fish health and food quality need to be considered. In the thesis, different substitutes of fishmeal and fish oil were evaluated by using NMR and MS-based metabolomics.

The Baltic Sea is one of the most threatened water bodies and has environmental problems, such as contamination and eutrophication. The use of Baltic Sea-sourced nutrients after certain treatments in fish feeds would recycle the less valuable nutrients for human back into the food chain, and may promote an environmental-friendly aquaculture system.

In the thesis, we found that use of detoxified fishmeal and fish oil reduced adverse effects on fish health related to energy metabolism and hepatotoxicity, compared with the untreated diets. Moreover, the decontaminated fish materials containing high content of n-3 fatty acids were found to be valuable sources of fish feeds. Additionally, a new Baltic blend diet composed of Baltic Sea-sourced decontaminated fishmeal, blue mussel and baker’s yeast was fed to Arctic char (Salvelinus alpinus) for 10 months. Based on the metabolomics results, the dietary content of betaine, trimethylamine-N-oxide and aromatic amino acids needs to be modified, in order to achieve a better growth performance. The hepatic metabolic heterogeneity of salmonids was also observed in the thesis.

Furthermore, the sphingolipids in salmonids skin were characterized for the first time. We found that reduction in dietary levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) changed the fatty acid composition in glycerol-phospholipids subclasses and sphingolipid composition in skin of Atlantic salmon (Salmo salar). These changes potentially disturb the barrier function of fish skin.

These findings provide new information on application of metabolomics in development of alternative aquafeeds.

Authors/Creators:Cheng, Ken
Title:Metabolomics as a tool to evaluate salmonid response to alternative feed ingredients
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :2017
Depositing date:6 March 2017
Volume:2017:33
Number of Pages:64
Papers/manuscripts:
NumberReferences
ICheng, K., Wagner, L., Pickova, J., & Moazzami, A.A. (2016). NMR-based metabolomics reveals compartmental metabolic heterogeneity in liver of Arctic char (Salvelinus alpinus). Canadian Journal of Zoology, 94(9), pp. 665-669.
IICheng, K., Wagner, L., Moazzami, A.A., Gómez-Requeni, P., Schiller Vestergren, A., Brännäs, E., Pickova, J., & Trattner, S. (2016). Decontaminated fishmeal and fish oil from the Baltic Sea are promising feed sources for Arctic char (Salvelinus alpinus L.) – Studies of flesh lipid quality and metabolic profile. European Journal of Lipid Science and Technology, 118(6), pp. 862-873.
IIICheng, K., Müllner, E., Moazzami, A.A., Carlberg, H., Brännäs, E., & Pickova, J. Metabolomics approach to evaluate a Baltic Sea-sourced diet for cultured Arctic char (Salvelinus alpinus L.). (submitted)
IVCheng, K., Mira, M.B., Du, L., Ruyter, B., Moazzami, A.A., Ehtesham, E., Venegas, C., & Pickova, J. Reducing dietary levels of EPA and DHA have major impacts on the composition of different skin membrane lipid classes of Atlantic salmon (Salmo salar L.). (manuscript)
Place of Publication:Uppsala
Publisher:Department of Molecular Sciences, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-8839-2
ISBN for electronic version:978-91-576-8840-8
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:Q Food science > Q04 Food composition
Q Food science > Q54 Feed composition
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Food Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Fish and Aquacultural Science
Agrovoc terms:saccharomyces cerevisiae, sphingolipids, mytilus edulis, fishes, metabolism, fatty acids, baltic sea
Keywords:Baltic Sea, ceramide, DHA, EPA, fatty acids, fish metabolism, glycerolphospholipids, Mytilus edulis, Saccharomyces cerevisiae, sphingolipids
URN:NBN:urn:nbn:se:slu:epsilon-e-4008
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-4008
ID Code:14133
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Molecular Sciences
External funders:China Scholarship Council
Deposited By: Ms Ken Cheng
Deposited On:14 Mar 2017 08:34
Metadata Last Modified:14 Mar 2017 14:50

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