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Smells of sociality

insect chemoreceptors, sex and habitat cues

Gonzalez, Francisco (2017). Smells of sociality. Diss. (sammanfattning/summary) Alnarp : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 ; 2017:39
ISBN 978-91-576-8851-4
eISBN 978-91-576-8852-1
[Doctoral thesis]

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The last two decades have seen considerable research effort dedicated to understanding the molecular basis of insect olfaction. There are, however, many knowledge gaps, especially when it comes to how insects detect different olfactory stimuli from the environment. In this thesis I aim to deepen our understanding of the detection of social and environmental cues in two insect species.

Codling moth, Cydia pomonella, is the foremost pest of apple. Following an initial identification of the chemoreceptors in male and female antennae, we provided an extensive annotation, transcript abundance quantification and repertoire completion of each of the three main chemoreceptor gene families. These results evidenced the importance of the candidate pheromone receptors (PRs), OR1, OR6 and OR3. Then, by heterologous expression in Drosophila melanogaster flies we functionally characterized some of these receptors. These experiments demonstrated that CpomOR3 is highly specific and sensitive toward pear ester, a strong kairomone and pheromone synergist of codling moth, which had not previously been found in apples. These results inspired a refined analysis of apple headspace which demonstrated the presence of this kairomone in apples. Furthermore, we show a putative microbial origin of this compound. We also characterized CpomOR6 as the receptor of the pheromone antagonist codlemone acetate, and CpomOR19 as a receptor tuned to indanones. Additionally, we provide a step-by-step description of the protocol to produce and characterize insect ORs and PRs through heterologous expression in D. melanogaster. Finally, we identified chemoreceptors from three tortricids and predict their function based on our results from codling moth. In addition, we investigated the common fruit fly D. melanogaster. In this species, an olfactory gene, OR69a, is expressed as two alternatively spliced variants: OR69aA and OR69aB. Through means of single sensillum recordings (SSRs) we characterized the non-overlapping response of both variants, showing that OR69aA responds to several plant compounds, while OR69aB detects a novel, long-range female-produced pheromone.

Together our results show that insects possess elegant ways to detect species-specific signals and the habitat cues that interact with those signals.

Authors/Creators:Gonzalez, Francisco
Title:Smells of sociality
Subtitle:insect chemoreceptors, sex and habitat cues
Series Name/Journal:Acta Universitatis Agriculturae Sueciae
Year of publishing :24 March 2017
Depositing date:13 March 2017
Number of Pages:66
IWilliam B. Walker, Francisco Gonzalez, Stephen F. Garczynski, and Peter Witzgall. (2016). "The chemosensory receptors of codling moth Cydia pomonella–expression in larvae and adults."Scientific Reports 6:23518.
IIJonas M. Bengtsson, Francisco Gonzalez, Alberto M. Cattaneo, Nicolas Montagné, William B. Walker, Marie Bengtsson, Gianfranco Anfora, Rickard Ignell, Emmanuelle Jacquin-Joly, and Peter Witzgall. (2014). "A predicted sex pheromone receptor of codling moth Cydia pomonella detects the plant volatile pear ester." Frontiers in Ecology and Evolution 2 (33).
IIIAlberto Maria Cattaneo, Francisco Gonzalez, Jonas M. Bengtsson, Elizabeth A. Corey, Emmanuelle Jacquin-Joly, Nicolas Montagné, Umberto Salvagnin, William B. Walker, Gianfranco Anfora, Peter Witzgall and Yuriy V. Bobkov. (2017). “Candidate pheromone receptors from the insect pest Cydia pomonella respond to pheromone and kairomone components”. Scientific Reports 7:41105.
IVFrancisco Gonzalez, Jonas M. Bengtsson, William B. Walker, Maria FR Sousa, Alberto M. Cattaneo, Nicolas Montagné, Arthur de Fouchier, Gianfranco Anfora, Emmanuelle Jacquin-Joly, Peter Witzgall and Rickard Ignell. (2015). "A Conserved Odourant Receptor Detects the Same 1-Indanone Analogs in a Tortricid and a Noctuid Moth." Frontiers in Ecology and Evolution 3 (131).
VFrancisco Gonzalez, Peter Witzgall, and William B. Walker. (2016)."Protocol for heterologous expression of insect odourant receptors in Drosophila." Frontiers in Ecology and Evolution 4 (24).
VIFrancisco Gonzalez, Maria Sousa, Lucie Conchou, William B. Walker, Amrita Chakraborty, Maria Karlsson, Göran Birgersson, Beatrix Alsanius, Alan Knight and Peter Witzgall. “Pear ester: the host cue that was not there”. Manuscript.
VIISebastien Lebreton, Felipe Borrero-Echeverry, Francisco Gonzalez, Marit Solum, Erika Wallin, Federica Trona, Amelie Baschwitz, Veit Grabe, Volker Jörger, Silke Sachse, Erik Hedenström, Bill S. Hansson, Anna-Lena Gustavsson, Marie Bengtsson, Göran Birgersson, William B. Walker, Hany Dweck, Paul Becher, Peter Witzgall. (2017). “The Drosophila pheromone Z4-11Al is encoded together with olfactory cues and mediates species-specific communication. bioRxiv 083071
VIIIFrancisco Gonzalez, Peter Witzgall and William B. Walker. (2017). “Antennal transcriptomes of three tortricid moths reveal conserved chemosensory receptors for social and habitat olfactory cues”. Scientific Reports 7:41829.
Place of Publication:Alnarp
Publisher:Department of Plant Protection Biology, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-8851-4
ISBN for electronic version:978-91-576-8852-1
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:H Protection of plants and stored products > H10 Pests of plants
L Animal production > L20 Animal ecology
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Ecology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Evolutionary Biology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Horticulture
Agrovoc terms:cydia pomonella, cydia nigricana, drosophila melanogaster, insecta, pest insects, olfaction, smell, pheromones, ecology, chemoreceptors, habitats
Keywords:Semiochemicals, Cydia pomonella, Drosophila melanogaster
Permanent URL:
ID Code:14165
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Protection Biology
Deposited By: M.Sc Francisco Gonzalez
Deposited On:15 Mar 2017 12:37
Metadata Last Modified:10 Sep 2020 13:41

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