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Genome-guided analysis and whole transcriptome profiling of the mesophilic syntrophic acetate oxidising bacterium Syntrophaceticus schinkii

Manzoor, Shahid and Bongcam-Rudloff, Erik and Schnürer, Anna and Müller, Bettina (2016). Genome-guided analysis and whole transcriptome profiling of the mesophilic syntrophic acetate oxidising bacterium Syntrophaceticus schinkii. PloS one. 11:11, 1-24
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Official URL: http://dx.doi.org/10.1371/journal.pone.0166520

Abstract

Syntrophaceticus schinkii is a mesophilic, anaerobic acterium capable of oxidising acetate to CO2 and H-2 in intimate association with a methanogenic partner, a syntrophic relationship which operates close to the energetic limits of microbial life. Syntrophaceticus schinkii has been identified as a key organism in engineered methane-producing processes relying on syntrophic acetate oxidation as the main methane-producing pathway. However, due to strict cultivation requirements and difficulties in reconstituting the thermodynamically unfavourable acetate oxidation, the physiology of this functional group is poorly understood. Genome-guided and whole transcriptome analyses performed in the present study provide new insights into habitat adaptation, syntrophic acetate oxidation and energy conservation. The working draft genome of Syntrophaceticus schinkii indicates limited metabolic capacities, with lack of organic nutrient uptake systems, chemotactic machineries, carbon catabolite repression and incomplete biosynthesis pathways. Ech hydrogenase, [ FeFe] hydrogenases, [ NiFe] hydrogenases, F1F0-ATP synthase and membrane-bound and cytoplasmic formate dehydrogenases were found clearly expressed, whereas Rnf and a predicted oxidoreductase/heterodisulphide reductase complex, both found encoded in the genome, were not expressed under syntrophic growth condition. A transporter sharing similarities to the high-affinity acetate transporters of aceticlastic methanogens was also found expressed, suggesting that Syntrophaceticus schinkii can potentially compete with methanogens for acetate. Acetate oxidation seems to proceed via the Wood-Ljungdahl pathway as all genes involved in this pathway were highly expressed. This study shows that Syntrophaceticus schinkii is a highly specialised, habitat-adapted organism relying on syntrophic acetate oxidation rather than metabolic versatility. By expanding its complement of respiratory complexes, it might overcome limiting bioenergetic barriers, and drive efficient energy conservation from reactions operating close to the thermodynamic equilibrium, which might enable S. schinkii to occupy the same niche as the aceticlastic methanogens. The knowledge gained here will help specify process conditions supporting efficient and robust biogas production and will help identify mechanisms important for the syntrophic lifestyle.

Authors/Creators:Manzoor, Shahid and Bongcam-Rudloff, Erik and Schnürer, Anna and Müller, Bettina
Title:Genome-guided analysis and whole transcriptome profiling of the mesophilic syntrophic acetate oxidising bacterium Syntrophaceticus schinkii
Series/Journal:PloS one (1932-6203)
Year of publishing :2016
Volume:11
Number:11
Page range:1-24
Number of Pages:24
Publisher:Public Library of Science
ISSN:1932-6203
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:P Natural resources > P06 Renewable energy resources
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Bioinformatics and Systems Biology (methods development to be 10203)
Keywords:Oxidation, Genome analysis, Genome databases, Comparative Genomics, Methanogens, Conservation of energy, Gene predictions
URN:NBN:urn:nbn:se:slu:epsilon-e-4133
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-4133
Additional ID:
Type of IDID
Web of Science (WoS)000387909300060
ID Code:14277
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
VH - Faculty of Veterinary Medicine and Animal Science
Department:(NL, NJ) > Dept. of Microbiology (until 161231)
(VH) > Dept. of Animal Breeding and Genetics
Deposited By: SLUpub Connector
Deposited On:27 Apr 2017 08:36
Metadata Last Modified:27 Apr 2017 08:36

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