Frank, J. A. and Arntzen, M. Ø. and Sun, Li and Hagen, L. H. and McHardy, A. C. and Horn, Svein and Eijsink, Vincent G. H. and Schnürer, Anna and Pope, Phillip B.
(2016).
Novel syntrophic populations dominate an ammonia-tolerant methanogenic microbiome.
mSystems. 1
:5
, 1-13
[Journal article]
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Available under License Creative Commons Attribution. 387kB |
Official URL: http://dx.doi.org/10.1128/mSystems.00092-16
Abstract
Biogas reactors operating with protein-rich substrates have high methane potential and industrial value; however, they are highly susceptible to process failure because of the accumulation of ammonia. High ammonia levels cause a decline in acetate-utilizing methanogens and instead promote the conversion of acetate via a two-step mechanism involving syntrophic acetate oxidation (SAO) to H2 and CO2, followed by hydrogenotrophic methanogenesis. Despite the key role of syntrophic acetate-oxidizing bacteria (SAOB), only a few culturable representatives have been characterized. Here we show that the microbiome of a commercial, ammonia-tolerant biogas reactor harbors a deeply branched, uncultured phylotype (unFirm_1) accounting for approximately 5% of the 16S rRNA gene inventory and sharing 88% 16S rRNA gene identity with its closest characterized relative. Reconstructed genome and quantitative metaproteomic analyses imply unFirm_1’s metabolic dominance and SAO capabilities, whereby the key enzymes required for acetate oxidation are among the most highly detected in the reactor microbiome. While culturable SAOB were identified in genomic analyses of the reactor, their limited proteomic representation suggests that unFirm_1 plays an important role in channeling acetate toward methane. Notably, unFirm_1-like populations were found in other high-ammonia biogas installations, conjecturing a broader importance for this novel clade of SAOB in anaerobic fermentations.
Authors/Creators: | Frank, J. A. and Arntzen, M. Ø. and Sun, Li and Hagen, L. H. and McHardy, A. C. and Horn, Svein and Eijsink, Vincent G. H. and Schnürer, Anna and Pope, Phillip B. |
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Title: | Novel syntrophic populations dominate an ammonia-tolerant methanogenic microbiome |
Series/Journal: | mSystems (2379-5077) |
Year of publishing : | 2016 |
Volume: | 1 |
Number: | 5 |
Page range: | 1-13 |
Number of Pages: | 13 |
Publisher: | American Society for Microbiology |
ISSN: | 2379-5077 |
Language: | English |
Publication Type: | Journal article |
Refereed: | Yes |
Article category: | Scientific peer reviewed |
Version: | Published version |
Copyright: | Creative Commons: Attribution 4.0 |
Full Text Status: | Public |
Agris subject categories.: | P Natural resources > P06 Renewable energy resources |
Subjects: | (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109) (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Bioinformatics and Systems Biology (methods development to be 10203) (A) Swedish standard research categories 2011 > 2 Engineering and Technology > 209 Industrial Biotechnology > Bioenergy |
Keywords: | anaerobic digestion, biogas, metagenomics, metaproteomics, syntrophic acetate oxidation |
URN:NBN: | urn:nbn:se:slu:epsilon-e-4141 |
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-4141 |
ID Code: | 14279 |
Faculty: | NJ - Fakulteten för naturresurser och jordbruksvetenskap |
Department: | (NL, NJ) > Dept. of Microbiology (until 161231) |
Deposited By: | SLUpub Connector |
Deposited On: | 28 Apr 2017 07:58 |
Metadata Last Modified: | 26 Oct 2020 01:42 |
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