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Novel syntrophic populations dominate an ammonia-tolerant methanogenic microbiome

Frank, J. A. and Arntzen, M. Ø. and Sun, Li and Hagen, L. H. and McHardy, A. C. and Horn, Svein and Eijsink, Vincent G. H. and Schnürer, Anna and Pope, Phillip B. (2016). Novel syntrophic populations dominate an ammonia-tolerant methanogenic microbiome. mSystems. 1:5, 1-13
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Official URL: http://dx.doi.org/10.1128/mSystems.00092-16

Abstract

Biogas reactors operating with protein-rich substrates have high methane potential and industrial value; however, they are highly susceptible to process failure because of the accumulation of ammonia. High ammonia levels cause a decline in acetate-utilizing methanogens and instead promote the conversion of acetate via a two-step mechanism involving syntrophic acetate oxidation (SAO) to H2 and CO2, followed by hydrogenotrophic methanogenesis. Despite the key role of syntrophic acetate-oxidizing bacteria (SAOB), only a few culturable representatives have been characterized. Here we show that the microbiome of a commercial, ammonia-tolerant biogas reactor harbors a deeply branched, uncultured phylotype (unFirm_1) accounting for approximately 5% of the 16S rRNA gene inventory and sharing 88% 16S rRNA gene identity with its closest characterized relative. Reconstructed genome and quantitative metaproteomic analyses imply unFirm_1’s metabolic dominance and SAO capabilities, whereby the key enzymes required for acetate oxidation are among the most highly detected in the reactor microbiome. While culturable SAOB were identified in genomic analyses of the reactor, their limited proteomic representation suggests that unFirm_1 plays an important role in channeling acetate toward methane. Notably, unFirm_1-like populations were found in other high-ammonia biogas installations, conjecturing a broader importance for this novel clade of SAOB in anaerobic fermentations.

Authors/Creators:Frank, J. A. and Arntzen, M. Ø. and Sun, Li and Hagen, L. H. and McHardy, A. C. and Horn, Svein and Eijsink, Vincent G. H. and Schnürer, Anna and Pope, Phillip B.
Title:Novel syntrophic populations dominate an ammonia-tolerant methanogenic microbiome
Series/Journal:mSystems (2379-5077)
Year of publishing :2016
Volume:1
Number:5
Page range:1-13
Number of Pages:13
Publisher:American Society for Microbiology
ISSN:2379-5077
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:P Natural resources > P06 Renewable energy resources
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Bioinformatics and Systems Biology (methods development to be 10203)
(A) Swedish standard research categories 2011 > 2 Engineering and Technology > 209 Industrial Biotechnology > Bioenergy
Keywords:anaerobic digestion, biogas, metagenomics, metaproteomics, syntrophic acetate oxidation
URN:NBN:urn:nbn:se:slu:epsilon-e-4141
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-4141
ID Code:14279
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Microbiology (until 161231)
Deposited By: SLUpub Connector
Deposited On:28 Apr 2017 07:58
Metadata Last Modified:28 Apr 2017 07:58

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