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Molecular, phylogenic, mass-spectrometry and decay analyses of copper tolerant phialophora species causing soft rot of wood

Karunasekera, Hasanthi (2017). Molecular, phylogenic, mass-spectrometry and decay analyses of copper tolerant phialophora species causing soft rot of wood. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2017:48
ISBN 978-91-576-8869-9
eISBN 978-91-576-8870-5
[Doctoral thesis]

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Abstract

Soft rot decay of treated wood in ground contact and loss of service life is an important economic issue worldwide. One of the most important and cosmopolitan fungal groups causing soft rot belong to the imperfect fungal anamorph genus Phialophora. An aim of this study was therefore to determine the molecular identity and phylogeny of several economically important Phialophora species isolated from copper-treated and untreated wood using PCR combined with sequencing. Strain compatibility and copper detoxification of Phialophora species/strains were also assessed in-vitro from both liquid and solid cultures. The identity of a number of so far unclassified Phialophora spp. A strains isolated from treated wood in-service were found as Phialocephala dimorphospora.

The ability of strongly (P. malorum, P. mutabilis) and weakly (Chaetomium globosum) copper tolerant soft rot fungi to degrade CuSO4 and micronized-Cu (MC) treated wood (birch/pine) was assessed using a modified ENV 807 decay test with vermiculate and sterile soil. A direct relationship between Cu-tolerance in-vitro and soft rot decay of Cu-treated wood was not found. Rather, the additive effect of copper binding to the wood and the chemical nature of the wood material (lignin type/level) appeared more important with the less Cu-tolerant fungi causing similar/greater mass losses than Cu-tolerant strains.

LC-MS/MS and MALDI-TOF MS/MS allowed characterization of total and extracellular (wall-bound) upregulated proteins produced by P. malorum while growing in Cu-supplemented growth medium. Despite P. malorum lacking a sequenced genome, it was possible using modern proteomics to characterize changes in global proteins and detect a number of unique as well as up-regulated and down-regulated proteins when grown in Cu-supplemented liquid media. Using MALDI-TOF MS/MS a number of cell wall/slime bound proteins were also shown up-regulated in response to growth in copper media and thereby likely involved in Cu-tolerance.

Authors/Creators:Karunasekera, Hasanthi
Title:Molecular, phylogenic, mass-spectrometry and decay analyses of copper tolerant phialophora species causing soft rot of wood
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :2017
Volume:2017:48
Number of Pages:62
Papers/manuscripts:
NumberReferences
IKarunasekera, H. and Daniel. G. (2013) Molecular identification and phylogenic analysis by sequencing rDNA of the copper-tolerant soft-rot Phialophora spp. International Biodeterioration & Biodegradation 82, 45-52.
IIKarunasekera, H. and Daniel. G. (2015) Phylogenic, molecular and decay analysis of Phialophora species causing soft rot of wood International Wood Products Journal 6 (4), 189-197.
IIIKarunasekera, H., Terziev. N. and Daniel. G. (2017) Does copper tolerance provide a competitive advantage for degrading copper treated wood by soft rot fungi? International Biodeterioration & Biodegradation 117, 105-114.
IVKarunasekera, H., Pettersson, J., Mi, J., Bergquist. J. and Daniel. G. (2017) Aspect of copper tolerance of the soft rot fungus Phialophora malorum grown in-vitro in copper supplemented media: Light, electron microscopy and global protein expression studies through proteomics approach (Submitted to International Biodeterioration & Biodegradation).
VDaniel, G., Volc. J., Halada, P. and Karunasekera, H. (2017) Aspects of copper tolerance of the soft rot fungus Phialophora malorum grown in in-vitro in copper supplemented media: Cell wall bound copper and detoxification (Manuscript).
Place of Publication:Uppsala
Publisher:Department of Forest Products, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-576-8869-9
ISBN for electronic version:978-91-576-8870-5
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:J Handling, transport, storage and protection of agricultural products > J12 Handling, transport, storage and protection of forest products
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Forest Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Wood Science
Agrovoc terms:copper, phialophora, fungi, biological properties, phylogeny, wood decay, molecular biology, analysis
Keywords:copper tolerance, LC-MS/MS, MALDI-TOF MS/MS, Phialophora molarum, Phialophora spp., proteomics, soft rot decay
URN:NBN:urn:nbn:se:slu:epsilon-e-4159
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-4159
ID Code:14289
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Products
Deposited By: Martina Andersson Löfqvist
Deposited On:08 May 2017 10:52
Metadata Last Modified:08 May 2017 10:52

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