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Insulin and the early bovine embryo

influences on in vitro development, gene expression, and morphology

Laskowski, Denise (2017). Insulin and the early bovine embryo. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 ; 2017:65
ISBN 978-91-7760-020-6
eISBN 978-91-7760-021-3
[Doctoral thesis]

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Metabolic imbalance is a problem in the dairy industry because the metabolic
demands of increased milk production can lead to decreased fertility, and more
knowledge about improving the management and physical conditions of the cow
(the links between fertility, nutrition, milking, and dry period) is needed. Insulin
is an important hormone regulating the energy balance in the body, and insulin
concentrations change in situations of energy deficiency or excess, both of which
are known to decrease fertility in cows as well as in humans. Hyperinsulinemia
is associated with decreased fertility by impairing the developmental potential
of embryos, but the underlying reasons for this remain unclear. Our aim was to
investigate insulin-induced changes on development, morphology and molecular
signature in bovine blastocysts on Day 8 (BC8).

An in vitro model was used and morphology and gene expression were
analysed by combining confocal microscopy and microarray-based
transcriptome studies. Blastocysts were produced in vitro according to standard
methods using oocytes that were supplemented with three different insulin levels
(INS10 =10 µg/ml; INS0.1= 0.1 µg/ml; INS0=control) during maturation. The
transcriptome profile of BC8 was obtained and embryo quality grades,
developmental stages and morphologies were further assessed in terms of Factin,
DNA, and active mitochondria. Significant differences were observed in
developmental rates and morphology after insulin exposure. The observed
changes were reflected by increased expression of genes involved in cell division
and structure, mitochondrial activation, lipid metabolism, and oxidative stress.

Combining all of the results, it was shown that elevated insulin impairs the
developmental potential of the embryo. This work contributes to new knowledge
about the molecular background of embryos developing under metabolic stress
conditions such as hyperinsulinemia. Moreover, the studies are of comparative
value for humans where impaired fertility is often related to metabolic disorders.

Authors/Creators:Laskowski, Denise
Title:Insulin and the early bovine embryo
Subtitle:influences on in vitro development, gene expression, and morphology
Series Name/Journal:Acta Universitatis Agriculturae Sueciae
Year of publishing :September 2017
Depositing date:16 August 2017
Number of Pages:116
ILaskowski, Denise; Sjunnesson, Ylva; Gustafsson, Hans; Humblot, Patrice; Andersson, Göran; and Båge Renée (2016). Insulin concentrations used in in vitro embryo production systems - a pilot study on insulin stability with an emphasis on concentrations measured in vivo. Acta Veterinaria Scandinavica 58 (Suppl.1): 66.
IILaskowski, Denise; Båge, Renée; Humblot, Patrice; Andersson, Göran; Sirard, Marc-André and Sjunnesson, Ylva (2017). Insulin during in vitro oocyte maturation has an impact on development, mitochondria, and cytoskeleton in bovine Day 8 blastocysts. Theriogenology 101, 15–25.
IIILaskowski, Denise; Sjunnesson, Ylva; Humblot, Patrice; Sirard, Marc- André; Andersson, Göran; Gustafsson, Hans and Båge, Renée (2016). In vitro bovine oocyte maturation changes blastocyst gene expression and developmental potential. Reproduction, Fertility and Development 29, 876-889.
IVLaskowski, Denise; Andersson, Göran; Humblot, Patrice; Sirard, Marc-André; Sjunnesson, Ylva; Ferreira, Christina; Pirro, Valentina; Båge, Renée (2017). Lipid profile of bovine blastocysts exposed to insulin during in vitro oocyte maturation. Reproduction, Fertility and Development (submitted manuscript).
Place of Publication:Uppsala
Publisher:Department of Clinical Sciences, Swedish University of Agricultural Sciences.
ISBN for printed version:978-91-7760-020-6
ISBN for electronic version:978-91-7760-021-3
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:L Animal production > L52 Animal physiology - Growth and development
L Animal production > L53 Animal physiology - Reproduction
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Agrovoc terms:dairy cows, in vitro embryo production, animal embryos, gene transcription, metabolic disorders, reproductive disorders, infertibility
Keywords:reproduction, metabolic imbalance, embryo metabolism, oocyte maturation, insulin
Permanent URL:
ID Code:14494
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
External funders:FORMAS
Deposited By: DVM Denise Laskowski
Deposited On:24 Aug 2017 06:45
Metadata Last Modified:09 Sep 2020 14:17

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