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The role of bovine seminal plasma in fertility

Nongbua, Thanapol (2017). The role of bovine seminal plasma in fertility. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2017:70
ISBN 978-91-7760-030-5
eISBN 978-91-7760-031-2
[Doctoral thesis]

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Abstract

Bovine seminal plasma (SP) is known to have an effect on spermatozoa and the female reproductive tract. The differences in fertility among individuals could be due to variations in SP composition and its effect on both spermatozoa and the inseminated female. Single Layer Centrifugation (SLC) has been shown to select the most robust spermatozoa from the ejaculate and can be used to separate spermatozoa from SP. The purpose of this thesis was to study the various effects of bovine SP. The thesis was divided into 4 studies: Study I was to determine the effects of SLC on post-thaw sperm quality. Study II investigated the effect of adding SP back to SP-free sperm samples. Study III investigated the effect of bovine SP on bovine endometrial epithelial cells (bEEC) in culture. Study IV determined the effect of season and SLC on sperm quality. Our results show that bull spermatozoa selected by SLC had a higher proportion with high mitochondrial membrane potential (MMP) and a higher superoxide production than controls. The SLC-selected samples had a higher proportion of spermatozoa with normal morphology and a lower proportion with bent tails than controls; they had better kinematics than controls. However, sperm viability and chromatin integrity were not different between treatments. Adding 5% bovine SP had a beneficial effect on sperm velocity. Moreover, there was a beneficial effect of adding 5% heterologous SP from high fertility bulls on sperm velocity but a deleterious effect on chromatin integrity. Total cell number and viability of bEEC after challenge with 1% SP from either high or low fertility bulls did not differ from controls. In contrast, challenge with 4% SP from high or low fertility bulls (300H or 300L) negatively affected bEEC cell number and viability. Challenge with 300L had a greater adverse effect than 300H. There were differences in semen characteristics and sperm morphology among seasons. However, sperm kinematics, viability, chromatin integrity and MMP were not different between seasons. In conclusion, these results indicated that SLC can be used to enhance bull sperm quality. Moreover, adding bovine SP prior to cryopreservation affected sperm quality depending on the proportion of SP and the fertility of the bull from which it came. Bovine SP had a negative effect on bEEC in both a dose-dependent and fertility–dependent manner. Season had a slight effect on sperm morphology. Further studies are needed to investigate the factors involved in the interaction between SP and spermatozoa or bEEC, such as differences in SP composition between low and high fertility bulls, and breed and age of bull, as well as their effects on fertility.

Keywords: bovine SP, fertility, proportion of SP, Uterus cell, sperm quality, Climate, frozen semen

Authors/Creators:Nongbua, Thanapol
Title:The role of bovine seminal plasma in fertility
Alternative abstract:
LanguageAbstract
Swedish

Det har visats att bovin seminalplasma (SP) påverkar spermier och de honliga reproduktionsorganen. Skillnaden i fertilitet mellan individer kan bero på variationer både i sammansättningen hos SP och i dess effekter på såväl spermier som den inseminerade honan. Single Layer Centrifugation (SLC) har visats selektera fram de mest robusta spermierna från ejakulatet, och kan användas för att separera spermier från SP. Syftet med denna avhandling var att studera de olika effekterna av bovin SP. Avhandlingen är uppdelad i fyra delstudier; i Studie I fastställdes effekterna av SLC på spermiekvaliteten efter upptining, Studie II undersökte effekten av åter tillsätta SP till SP-fria spermieprover. Studie III undersökte effekten av bovin SP på cellkulturer av bovina epitelceller från endometriet (bEEC). Studie IV fastställde effekten av årstid och SLC på spermiekvaliteten hos tjurspermier i Thailand. Våra resultat visar att tjurspermier som selekterats med SLC hade en högre andel med hög mitokondriemembranpotential (MMP) och en högre superoxidproduktion än kontroller. De SLC-selekterade proverna hade en högre andel spermier med normal morfologi och en lägre andel med svansböjning än kontroller, dessutom hade de bättre kinematik än kontrollerna. Dock fanns det ingen skillnad i membranintegritet och kromatinintegritet mellan behandlingarna. Tillsats av 5% bovin SP hade en fördelaktig effekt på spermiernas hastighet. Dessutom fanns det en fördelaktig effekt av att tillsätta 5% heterolog SP från tjurar med hög fertilitet på spermiernas hastighet, men detta försämrade samtidigt kromatinintegriteten. Totalt cellantal och viabilitet hos bEEC efter exponering för 1% SP skilde sig inte från kontroller, oavsett om SP från hög- eller lågfertila tjurar användes. I motsats till detta gav exponering med 4% SP från hög- eller lågfertila tjurar (300H eller 300L) en negativ effekt på cellantal och viabilitet hos bEEC. Exponering med 300L gav en större negativ effekt än 300H. Det fanns skillnader mellan årstider i spermans karakteristika och spermiemorfologin. Dock skilde sig inte spermiernas kinematik, membranintegritet, kromatinintegritet eller MMP mellan årstiderna. Sammanfattningsvis indikerar dessa resultat att SLC kan användas för att förbättra kvaliteten hos prover av tjurspermier. Dessutom visades att en tillsats av SP innan nedfrysning påverkade spermiekvaliteten beroende på andelen SP som tillsattes och fertiliteten hos donatortjuren. Bovin SP hade en negativ effekt på bEEC som var både dos- och fertilitetsberoende. Årstiden hade en mindre effekt på spermiemorfologin. Ytterligare studier behövs för att undersöka de faktorer som är inblandade i samspelet mellan SP och spermier eller bEEC, såsom skillnader i sammansättningen hos SP mellan låg- och högfertila tjurar, ras och ålder hos tjurarna samt faktorernas effekter på fertiliteten.

Nyckelord: bovin SP, fertilitet, andel SP, livmodercell, spermiekvalitet, klimat, fryst sperma

Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :2017
Depositing date:20 September 2017
Volume:2017:70
Number of Pages:87
Papers/manuscripts:
NumberReferences
INongbua, T., Johannisson, A., Edman, A. & Morrell, J.M.* (2017). Effects of single layer centrifugation (SLC) on bull spermatozoa prior to freezing on post-thaw semen characteristics. Reproduction in Domestic Animals, 52, pp. 596-602. II
IINongbua, T.*, Al-Essawe, E., Johannisson, A., Edman, A. & Morrell, J.M. Effect of adding bovine seminal plasma prior to cryopreservation on bull sperm quality after thawing: part I and part II (Submitted) .
IIINongbua, T.*, Guo, Y., Edman, A., Humblot, P. & Morrell, J.M. Effect of bovine seminal plasma on bovine endometrial epithelial cells in culture (Accepted)
IVNongbua, T.*, Utta, A., Am-in, N., Suwimonteerabutr, J., Johannisson, A. & Morrell, J.M. Effects of season and Single Layer Centrifugation on bull sperm quality in Thailand (Submitted)
Place of Publication:Uppsala
Publisher:Department of Clinical Sciences, Swedish University of Agricultural Science
ISBN for printed version:978-91-7760-030-5
ISBN for electronic version:978-91-7760-031-2
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Subjects:Obsolete subject words > VETERINARY MEDICINE
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 402 Animal and Dairy Science > Animal and Dairy Science.
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Medical Bioscience
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Agrovoc terms:bovinae, fertility, seminal plasma
Keywords:bovine seminal plasma, fertility, proportion of SP, uterus cell, sperm quality, frozen semen, climate
URN:NBN:urn:nbn:se:slu:epsilon-e-4372
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-4372
ID Code:14569
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
External funders:FORMAS
Deposited By: Mr Thanapol Nongbua
Deposited On:21 Sep 2017 08:20
Metadata Last Modified:27 Sep 2017 07:24

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