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tRNA-derived small RNAs target transposable element transcripts

Martinez Arias, German and Slotkin, R. Keith and Choudury, Sarah G. (2017). tRNA-derived small RNAs target transposable element transcripts. Nucleic acids research. 45:9, 5142-5152
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Official URL: http://dx.doi.org/10.1093/nar/gkx103

Abstract

tRNA-derived RNA fragments (tRFs) are 18-26 nucleotide small RNAs that are not random degradation products, but are rather specifically cleaved from mature tRNA transcripts. Abundant in stressed or viral-infected cells, the function and potential targets of tRFs are not known. We identified that in the unstressed wild-type male gamete containing pollen of flowering plants, and analogous reproductive structure in non-flowering plant species, tRFs accumulate to high levels. In the reference plant Arabidopsis thaliana, tRFs are processed by Dicer-like 1 and incorporated into Argonaute1 (AGO1), akin to a microRNA. We utilized the fact that many plant small RNAs direct cleavage of their target transcripts to demonstrate that the tRF-AGO1 complex acts to specifically target and cleave endogenous transposable element (TE) mRNAs produced from transcriptionally active TEs. The data presented here demonstrate that tRFs are bona-fide regulatory microRNA-like small RNAs involved in the regulation of genome stability through the targeting of TE transcripts.

Authors/Creators:Martinez Arias, German and Slotkin, R. Keith and Choudury, Sarah G.
Title:tRNA-derived small RNAs target transposable element transcripts
Series/Journal:Nucleic acids research (1362-4962)
Year of publishing :2017
Volume:45
Number:9
Page range:5142-5152
Number of Pages:11
Publisher:Oxford Univeristy Press
ISSN:1362-4962
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:F Plant production > F30 Plant genetics and breeding
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding
Keywords:angiosperms, arabidopsis, dna transposable elements, germ cells
URN:NBN:urn:nbn:se:slu:epsilon-e-4389
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-4389
Additional ID:
Type of IDID
Web of Science (WoS)000402064200021
ID Code:14588
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Plant Biology (from 140101)
Deposited By: SLUpub Connector
Deposited On:03 Oct 2017 11:02
Metadata Last Modified:03 Oct 2017 11:02

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