Home About Browse Search
Svenska


Neuropeptidergic regulation of mosquito host-seeking behaviour

Christ, Peter (2017). Neuropeptidergic regulation of mosquito host-seeking behaviour. Diss. (sammanfattning/summary) Alnarp : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2017:96
ISBN 978-91-7760-082-4
eISBN 978-91-7760-083-1
[Doctoral thesis]

[img]
Preview
PDF
1MB

Abstract

Mosquitoes are vectors of numerous pathogens that cause human diseases, putting
more than half of the world’s population at risk. These diseases are transmitted when a
mosquito takes a blood meal following the successful seeking of a human host. Hostseeking
is highly state dependent and predominantly mediated by olfactory cues. The
goal of this thesis is to describe the neuropeptidergic regulation of the state dependent
odour-mediated host-seeking behaviour.
In order to identify and characterize the neuropeptides that are modulated by feeding
and are involved in host-seeking, I analysed the antennal lobes, the primary olfactory
centre, of Aedes aegypti, using semi-quantitative MALDI-TOF mass spectrometry.
Functional evidence for the involvement of the identified neuropeptides in the
regulation of host-seeking was provided using neuropeptide injections. I demonstrated
that short neuropeptide F-2 (sNPF-2) and allatostatin-A-5 (AstA-5) are regulated upon
blood feeding and that the injection of a binary mix of sNPF-2 and AstA-5 inhibited
host-seeking in non-blood fed mosquitoes, mimicking the effect of a blood meal. I next
characterized the sNPF and AstA receptors (sNPFR and AstAR) from Ae. aegypti and
two other important disease vectors, Culex quinquefasciatus and Anopheles coluzzii
assessing the receptor conservation and function as well as the regulation of the
receptors in response to blood feeding. Within the AstA signalling system, I described a
dipteran-specific duplication of the AstARs (R1 and R2) in mosquitoes. Functional
characterization revealed that the AstAR2s show a higher sensitivity to AstAs
compared to AstAR1s in the culicine mosquitoes Ae.aegypti and Cx. quinquefasciatus.
In contrast, both AstARs in An. coluzzii showed a similar sensitivity to the AstA
ligands, which suggests a divergence in the AstA signalling in mosquitoes. This is in
contrast to the sNPFRs in the three species, which showed a high conservation in
structure and receptor sensitivity. Blood feeding results in a selective regulation of
transcript abundance of the more sensitive AstAR2 and the sNPFR in Cx.
quinquefasciatus, but not in Ae. aegypti or An. coluzzii. This is indicative of differences
in the regulatory mechanisms for AstA and sNPF in Cx. quinquefasciatus compared
with the other species.
In this thesis, I provide strong evidence that host-seeking is regulated by complex
mechanisms involving at least two neuropeptidergic systems. These findings may shed
new light on previous results and should encourage further investigation of other
neuropeptide families. The functional characterization of the AstA and sNPF receptors
leads to a better understanding of the conservation and regulation of neuropeptide
signalling system and provides new targets for future research.

Authors/Creators:Christ, Peter
Title:Neuropeptidergic regulation of mosquito host-seeking behaviour
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :6 October 2017
Volume:2017:96
Number of Pages:82
Papers/manuscripts:
NumberReferences
IPeter Christ, Anna Reifenrath, Jörg Kahnt, Frank Hauser, Sharon R. Hill, Joachim Schachtner, Rickard Ignell. Feeding-induced changes in allatostatin-A and short neuropeptide F in the antennal lobes affect odormediated host seeking in the yellow fever mosquito, Aedes aegypti. submitted
IIPeter Christ, Sharon R. Hill, Joachim Schachtner, Frank Hauser, Rickard Ignell. Functional characterization of the dual allatostatin-A receptors in mosquitoes. Peptides, accepted with minor revisions
IIIPeter Christ, Sharon R. Hill, Joachim Schachtner, Frank Hauser, Rickard Ignell. Functional characterization of mosquito sNPF receptors. manuscript
Place of Publication:Alnarp
Publisher:Department of Plant Protection Biology, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-7760-082-4
ISBN for electronic version:978-91-7760-083-1
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:X Agricola extesions > X30 Life sciences
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Behavioural Sciences Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Other Biological Topics
Agrovoc terms:Culicidae, neuropeptides, olfaction, feeding habits
Keywords:olfaction,, modulation,, neuropeptides,, short neuropeptide F, allatostatin-A, host-seeking, sugar feeding, behaviour, G protein coupled receptors
URN:NBN:urn:nbn:se:slu:epsilon-e-4398
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-4398
ID Code:14608
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Protection Biology
Deposited By: Peter Christ
Deposited On:05 Oct 2017 13:44
Metadata Last Modified:05 Oct 2017 13:44

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits