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Micropropagation, molecular and biochemical analyses of Hypoxis species

Nsibande, Busie E.B. (2017). Micropropagation, molecular and biochemical analyses of Hypoxis species. Diss. (sammanfattning/summary) Alnarp : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 ; 2017:94
ISBN 978-91-7760-078-7
eISBN 978-91-7760-079-4
[Doctoral thesis]

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Plants are an important source of food, nutrition and medicine. An estimated 80% of the global population relies on herbal medicinal products and supplements. In developing countries plant extracts are the major source of medicine, more especially in rural communities where modern health facilities are not easily accessible. Hypoxis species are associated with medicinal properties and have been used for many generations by the indigenous people and traditional healers of southern Africa to treat various ailments. Some contain hypoxoside, a phytochemical that has immune regulatory properties and is used in the composition of medicinal products in the pharmaceutical industry. Hypoxis species grow naturally in most warm temperate and tropical regions of the world except Europe, northern and central Asia, north Africa, extra-tropical South America and Canada, and are reported to be mostly common in the southern hemisphere.
The objectives of this work were to develop strategies for the sustainable commercial production and maintenance of the medicinal Hypoxis species in the near future, to understand how the different species that grow in Swaziland are related, and to determine the phytochemical compounds of medicinal significance by i) developing in vitro protocols for their propagation and mass production; ii) studying the molecular phylogeny of some Hypoxis species that grow in Swaziland and iii) analyzing the geophytes for the presence of important biochemical compounds known for their medicinal value.
The investigations were carried out at the Swedish University of Agricultural Sciences (SLU), Department of Plant Breeding in Alnarp, Sweden. All species used in this work originated from Swaziland and were identified at the National Herbarium in Swaziland. The major results include development of in vitro regeneration protocols for propagating Hypoxis on semi-solid media and in liquid medium using bioreactors for mass production, identification of two taxa with a similar evolutionary relationship, extracts from the corms of all the species analyzed were found to contain phenolic compounds and the norlignan diglucoside, hypoxoside. However, in this study, H. hemerocallidea the main species used in the pharmaceutical industry for extracting hypoxoside was found to contain less of it compared to most of the other species analyzed.

Authors/Creators:Nsibande, Busie E.B.
Title:Micropropagation, molecular and biochemical analyses of Hypoxis species
Series Name/Journal:Acta Universitatis Agriculturae Sueciae
Year of publishing :30 October 2017
Depositing date:30 October 2017
Number of Pages:43
INsibande Busie, Li Xue-Yuan, Ahlman Annelie and Zhu Li-Hua. (2015). In vitro Regeneration of Endangered Medicinal Hypoxis Species. American Journal of Plant Sciences 6 (16), 2585-95.
IINsibande Busie and Zhu Li-Hua (2017). Developing an in vitro Propagation Method for Mass Production of Medicinal Hypoxis Species Using Bioreactors. Asian Journal of Science and Research 6 (3), 52-59.
IIINsibande Busie, Geleta Mulatu and Zhu Li-Hua. Molecular Phylogeny of Some Hypoxis Inferred through Sequencing of Four Chloroplast DNA Regions (manuscript).
IVNsibande Busie, Gustavsson Carl-Erik and Zhu Li-Hua. Analysis of Some Phytochemical Compounds in Seven Species of Hypoxis (submitted manuscript).
Place of Publication:Alnarp
Publisher:Department of Plant Breeding, Swedish University of Agricultural Sciences.
ISBN for printed version:978-91-7760-078-7
ISBN for electronic version:978-91-7760-079-4
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F02 Plant propagation
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding
Agrovoc terms:hypoxis, medicinal plants, bioreactors, chloroplast DNA, corms, chemical composition, micropropagation, phenolic compounds, indigineous knowledge, Swaziland
Keywords:Bioreactor, Chloroplast DNA, Corm, Hypoxis, Hypoxoside, Micropropagation
Permanent URL:
ID Code:14673
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
External funders:Swedish International Development Cooperation Agency
Deposited By: Mrs Busie E.B Nsibande
Deposited On:31 Oct 2017 12:59
Metadata Last Modified:09 Sep 2020 14:17

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