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Fish and amphibians as test organisms for evaluation of effects caused by chemicals

Carlsson, Gunnar (2007). Fish and amphibians as test organisms for evaluation of effects caused by chemicals. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2007:61
ISBN 978-91-576-7360-2
[Doctoral thesis]

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Abstract

A large number of chemicals can contaminate aquatic environments and therefore be exposed to fish and amphibians during their sensitive stages of development. This raises the need for robust methods to identify chemicals that disturb the developmental process. In this thesis, methods for toxicity testing and biomonitoring were developed for zebrafish (Danio rerio) and West-African clawed frog (Xenopus tropicalis). Using these methods, two groups of substances that have achieved attention during recent years were tested, synthetic musks and brominated flame retardants, as well as substances with known mechanism of action. Moreover, zebrafish embryos were used to evaluate chemically complex extracts prepared of effluent water from oil/gas production platforms. Exposure was performed on the embryo stages, to reveal embryo toxic endpoints and in connection to the metamorphosis process in frogs, to evaluate disturbances of the thyroid hormone system. Both methods were able to detect adverse effects in exposed animals. The studies showed that some musk substances had toxic effects on embryos in environmentally relevant concentrations. Embryo toxic responses of musk ketone (MK) and tetrabromobisphenol-A (TBBPA) were recorded in zebrafish as well as in Xenopus tropicalis and moor frog (Rana arvalis) at comparable concentrations. Zebrafish embryos were adequate for monitoring the toxic impact of effluent water from oil/gas production platforms. Effects on X. tropicalis tadpoles due to exposure to propylthiouracil were reduced development and decreased hind limb length, which can be explained by thyroid disruption. Increased sensitivity of the method was achieved by measurements on histological preparations of the thyroid glands. Exposure to polybrominated diphenylethers resulted in signs of thyroid disrupting properties of one tested congener, BDE-99. Moreover, distribution of BDE-99 in tadpole and juvenile X. tropicalis showed long-term retention and accumulation in adipose tissue.

Authors/Creators:Carlsson, Gunnar
Title:Fish and amphibians as test organisms for evaluation of effects caused by chemicals
Year of publishing :May 2007
Volume:2007:61
Number of Pages:42
Papers/manuscripts:
NumberReferences
ALLCarlsson, G. & Norrgren, L. (2004). Synthetic Musk Toxicity to Early Life Stages of Zebrafish (Danio rerio). Archives of Environmental Contamination and Toxicology 46, 102-105. Carlsson, G. & Norrgren, L. (2007). Embryo toxicity using different classes of aquatic vertebrates. Submitted to Archives of Environmental Contamination and Toxicology. Carlsson, G., Norrgren, L., Hylland, K. & Tollefsen, KE. (2007). Effluents from oil production platforms in the North Sea cause developmental toxicity to zebrafish (Danio rerio) embryos. Manuscript. Carlsson, G. & Norrgren, L. (2007). The impact of the goitrogen 6-propylthiouracil (PTU) on West-African clawed frog (Xenopus tropicalis) exposed during metamorphosis. Aquatic Toxicology 82, 55-62. Carlsson, G., Kulkarni, P., Larsson, P. & Norrgren, L. (2007). Distribution of BDE-99 and effects on metamorphosis of BDE-99 and -47 after oral exposure in Xenopus tropicalis. Submitted to Aquatic Toxicology.
Place of Publication:Uppsala
ISBN for printed version:978-91-576-7360-2
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:fishes, amphibians, aquatic environment, pollution, environmental impact, chemical contamination, toxicity, testing, monitoring
Keywords:zebrafish, Danio rerio, Xenopus tropicalis, embryo, toxicity, tadpole, thyroid, musks, brominated flame retardants
URN:NBN:urn:nbn:se:slu:epsilon-1616
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-1616
ID Code:1470
Department:(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
Deposited By: Gunnar Carlsson
Deposited On:23 May 2007 00:00
Metadata Last Modified:02 Dec 2014 10:12

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