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Improvement of identification methods for honeybee specific Lactic Acid Bacteria; future approaches.

Lamei, Sepideh and Hu, Yue O. O. and Olofsson, Tobias and Andersson, Anders F. and Forsgren, Eva and Vasquez, Alejandra (2017). Improvement of identification methods for honeybee specific Lactic Acid Bacteria; future approaches. PloS one. 12, 1-12
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Official URL: https://doi.org/10.1371/journal.pone.0174614

Abstract

Honeybees face many parasites and pathogens and consequently rely on a diverse set of individual and group-level defenses to prevent disease. The crop microbiota of Apis mellifera, composed of 13 Lactic Acid Bacterial (LAB) species within the genera Lactobacillus and Bifidobacterium, form a beneficial symbiotic relationship with each other and the honeybee to protect their niche and their host. Possibly playing a vital role in honeybee health, it is important that these honeybee specific Lactic Acid Bacterial (hbs-LAB) symbionts can be correctly identified, isolated and cultured, to further investigate their health promoting properties. We have previously reported successful identification to the strain level by culture-dependent methods and we recently sequenced and annotated the genomes of the 13 hbs-LAB. However, the hitherto applied techniques are unfortunately very time consuming, expensive and not ideal when analyzing a vast quantity of samples. In addition, other researchers have constantly failed to identify the 13 hbs-LAB from honeybee samples by using inadequate media and/or molecular techniques based on 16S rRNA gene sequencing with insufficient discriminatory power. The aim of this study was to develop better and more suitable methods for the identification and cultivation of hbs-LAB. We compared currently used bacterial cultivation media and could for the first time demonstrate a significant variation in the hbs-LAB basic requirements for optimal growth. We also present a new bacterial identification approach based on amplicon sequencing of a region of the 16S rRNA gene using the Illumina platform and an error correction software that can be used to successfully differentiate and rapidly identify the 13 hbs-LAB to the strain level.

Authors/Creators:Lamei, Sepideh and Hu, Yue O. O. and Olofsson, Tobias and Andersson, Anders F. and Forsgren, Eva and Vasquez, Alejandra
Title:Improvement of identification methods for honeybee specific Lactic Acid Bacteria; future approaches.
Series/Journal:PloS one (1932-6203)
Year of publishing :2017
Volume:12
Page range:1-12
Number of Pages:12
Publisher:Public Library of Science
ISSN:1932-6203
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:L Animal production > L72 Pests of animals
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
Agrovoc terms:honey bees, lactic acid bacteria, parasites
Keywords:honey bees, Apis mellifera, Lactic Acid Bacteria, parasites, pathogenes, symbiosis
URN:NBN:urn:nbn:se:slu:epsilon-e-4550
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-4550
Additional ID:
Type of IDID
DOI10.1371/journal.pone.0174614
Web of Science (WoS)000399174300061
ID Code:14738
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Ecology
(S) > Dept. of Ecology
Deposited By: SLUpub Connector
Deposited On:11 Dec 2017 09:46
Metadata Last Modified:11 Dec 2017 09:46

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