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Methane Production in Dairy Cows Correlates with Rumen Methanogenic and Bacterial Community Structure

Danielsson, Rebecca and Dicksved, Johan and Sun, Li and Gonda, Horacio and Müller, Bettina and Schnürer, Anna and Bertilsson, Jan (2017). Methane Production in Dairy Cows Correlates with Rumen Methanogenic and Bacterial Community Structure. Frontiers in Microbiology. 8, 1-15
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Official URL: http://dx.doi.org/10.3389/fmicb.2017.00226

Abstract

Methane (CH4) is produced as an end product from feed fermentation in the rumen. Yield of CH4 varies between individuals despite identical feeding conditions. To get a better understanding of factors behind the individual variation, 73 dairy cows given the same feed but differing in CH4 emissions were investigated with focus on fiber digestion, fermentation end products and bacterial and archaeal composition. In total 21 cows (12 Holstein, 9 Swedish Red) identified as persistent low, medium or high CH4 emitters over a 3 month period were furthermore chosen for analysis of microbial community structure in rumen fluid. This was assessed by sequencing the V4 region of 16S rRNA gene and by quantitative qPCR of targeted Methanobrevibacter groups. The results showed a positive correlation between low CH4 emitters and higher abundance of Methanobrevibacter ruminantium clade. Principal coordinate analysis (PCoA) on operational taxonomic unit (OTU) level of bacteria showed two distinct clusters (P < 0.01) that were related to CH4 production. One cluster was associated with low CH4 production (referred to as cluster L) whereas the other cluster was associated with high CH4 production (cluster H) and the medium emitters occurred in both clusters. The differences between clusters were primarily linked to differential abundances of certain OTUs belonging to Prevotella. Moreover, several OTUs belonging to the family Succinivibrionaceae were dominant in samples belonging to cluster L. Fermentation pattern of volatile fatty acids showed that proportion of propionate was higher in cluster L, while proportion of butyrate was higher in cluster H. No difference was found in milk production or organic matter digestibility between cows. Cows in cluster L had lower CH4/kg energy corrected milk (ECM) compared to cows in cluster H, 8.3 compared to 9.7 g CH4/kg ECM, showing that low CH4 cows utilized the feed more efficient for milk production which might indicate a more efficient microbial population or host genetic differences that is reflected in bacterial and archaeal (or methanogens) populations.

Authors/Creators:Danielsson, Rebecca and Dicksved, Johan and Sun, Li and Gonda, Horacio and Müller, Bettina and Schnürer, Anna and Bertilsson, Jan
Title:Methane Production in Dairy Cows Correlates with Rumen Methanogenic and Bacterial Community Structure
Series/Journal:Frontiers in Microbiology (1664-302X)
Year of publishing :2017
Volume:8
Page range:1-15
Number of Pages:15
Publisher:Frontiers Media
ISSN:1664-302X
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:L Animal production > L20 Animal ecology
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 402 Animal and Dairy Science > Animal and Dairy Science.
Keywords:rumen, CH4, microbial community, Methanobrevibacter, digestibility
URN:NBN:urn:nbn:se:slu:epsilon-e-4586
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-4586
Additional ID:
Type of IDID
Web of Science (WoS)000394279100001
ID Code:14781
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
VH - Faculty of Veterinary Medicine and Animal Science
Department:(NL, NJ) > Dept. of Microbiology (until 161231)
(VH) > Dept. of Animal Nutrition and Management
Deposited By: SLUpub Connector
Deposited On:22 Dec 2017 06:47
Metadata Last Modified:22 Dec 2017 06:47

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