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Proteome analysis of bronchoalveolar lavage from calves infected with bovine respiratory syncytial virus-Insights in pathogenesis and perspectives for new treatments

Hägglund, Sara and Blodörn, Krister and Näslund, Katarina and Vargmar, Karin and Bergström Lind, Sara and Mi, Jia and Araínga, Mariluz and Riffault, Sabine and Talyor, Geraldine and Pringle, John and Valarcher, Jean-Francois (2017). Proteome analysis of bronchoalveolar lavage from calves infected with bovine respiratory syncytial virus-Insights in pathogenesis and perspectives for new treatments. PloS one. 12:10, 1-19
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Official URL: http://dx.doi.org/10.1371/journal.pone.0186594

Abstract

Human and bovine respiratory syncytial viruses (HRSV/BRSV) are major causes of severe lower respiratory tract infections in children and calves, respectively. Shared epidemiological, clinical, pathological and genetic characteristics of these viruses make comparative research highly relevant. To characterise the host response against BRSV infection, bronchoalveolar lavage supernatant (BAL) from i) non-vaccinated, BRSV-infected ii) vaccinated, BRSV-infected and iii) non-infected calves was analysed by tandem mass spectrometry. Proteins were semi-quantified and protein expression was validated by immunoblotting. Correlations between selected proteins and pathology, clinical signs and virus shedding were investigated. Calves with BRSV-induced disease had increased total protein concentrations and a decreased number of proteins identified in BAL. The protein profile was characterised by neutrophil activation and a reduction in identified antioxidant enzymes. The presence of neutrophils in alveolar septa, the expression level of neutrophil-related or antioxidant proteins and LZTFL1 correlated significantly with disease. Citrullinated histone 3, an indicator of extracellular traps (ETs), was only detected in non-vaccinated, BRSV-infected animals. By bringing disequilibrium in the release and detoxification of reactive oxygen species, generating ETs and causing elastine degradation, exaggerated neutrophil responses might exacerbate RSV-induced disease. Neutrophil-mitigating or antioxidant treatments should be further explored.

Authors/Creators:Hägglund, Sara and Blodörn, Krister and Näslund, Katarina and Vargmar, Karin and Bergström Lind, Sara and Mi, Jia and Araínga, Mariluz and Riffault, Sabine and Talyor, Geraldine and Pringle, John and Valarcher, Jean-Francois
Title:Proteome analysis of bronchoalveolar lavage from calves infected with bovine respiratory syncytial virus-Insights in pathogenesis and perspectives for new treatments
Series/Journal:PloS one (1932-6203)
Year of publishing :2017
Volume:12
Number:10
Page range:1-19
Number of Pages:19
Publisher:Public Library of Science
ISSN:1932-6203
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Agris subject categories.:L Animal production > L70 Veterinary science and hygiene - General aspects
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Agrovoc terms:Bovine respiratory syncytial virus, calves, epidemiology, spectrometry
Keywords:Bovine respiratory syncytial virus, calves, epidemiology, spectrometry
URN:NBN:urn:nbn:se:slu:epsilon-e-4620
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-4620
Additional ID:
Type of IDID
Web of Science (WoS)000413054800048
ID Code:15036
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
Deposited By: SLUpub Connector
Deposited On:15 Jan 2018 07:05
Metadata Last Modified:15 Jan 2018 07:05

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